BAG2 Antibody - #DF2650

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Product: BAG2 Antibody
Catalog: DF2650
Description: Rabbit polyclonal antibody to BAG2
Application: WB IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Bovine, Sheep, Rabbit, Dog
Mol.Wt.: 24 kDa; 24kD(Calculated).
Uniprot: O95816
RRID: AB_2839856

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Bovine(90%), Sheep(90%), Rabbit(100%), Dog(80%)
Clonality:
Polyclonal
Specificity:
BAG2 Antibody detects endogenous levels of total BAG2.
RRID:
AB_2839856
Cite Format: Affinity Biosciences Cat# DF2650, RRID:AB_2839856.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

BAG 2; BAG family molecular chaperone regulator 2; BAG-2; BAG-family molecular chaperone regulator-2; Bag2; BAG2_HUMAN; BCL 2 associated athanogene 2; Bcl-2-associated athanogene 2; BCL2 associated athanogene 2; BCL2-associated athanogene 2; dJ417I1.2 (BAG family molecular chaperone regulator 2); dJ417I1.2; KIAA0576; MGC149462; OTTHUMP00000016668;

Immunogens

Immunogen:
Uniprot:

O95816(BAG2_HUMAN) >>Visit HPA database.

Gene(ID):
BAG2(9532)
Description:
Inhibits the chaperone activity of HSP70/HSC70 by promoting substrate release.;
Sequence:
MAQAKINAKANEGRFCRSSSMADRSSRLLESLDQLELRVEALREAATAVEQEKEILLEMIHSIQNSQDMRQISDGEREELNLTANRLMGRTLTVEVSVETIRNPQQQESLKHATRIIDEVVNKFLDDLGNAKSHLMSLYSACSSEVPHGPVDQKFQSIVIGCALEDQKKIKRRLETLLRNIENSDKAIKLLEHSKGAGSKTLQQNAESRFN

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Rabbit
100
Bovine
90
Sheep
90
Dog
80
Horse
70
Pig
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O95816 As Substrate

Site PTM Type Enzyme
A2 Acetylation
K5 Ubiquitination
K9 Ubiquitination
S20 Phosphorylation P49137 (MAPKAPK2)
S31 Phosphorylation
S73 Phosphorylation
K111 Acetylation
K111 Ubiquitination
K123 Ubiquitination
S133 Phosphorylation
S137 Phosphorylation
Y139 Phosphorylation
S140 Phosphorylation
S143 Phosphorylation
S144 Phosphorylation
K154 Ubiquitination
C162 S-Nitrosylation
K168 Acetylation
K168 Ubiquitination
K169 Acetylation
T176 Phosphorylation
K186 Acetylation
K186 Ubiquitination
K189 Ubiquitination
K195 Ubiquitination
K200 Ubiquitination
T201 Phosphorylation

Research Backgrounds

Function:

Co-chaperone for HSP70 and HSC70 chaperone proteins. Acts as a nucleotide-exchange factor (NEF) promoting the release of ADP from the HSP70 and HSC70 proteins thereby triggering client/substrate protein release.

Subunit Structure:

Binds to the ATPase domain of HSP/HSC70 chaperones. May interact with NWD1. Interacts with HSPA1A (via NBD), HSPA1B (via NBD) and HSPA8.

Research Fields

· Genetic Information Processing > Folding, sorting and degradation > Protein processing in endoplasmic reticulum.   (View pathway)

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