DDR2 Antibody - #DF2663

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Product: DDR2 Antibody
Catalog: DF2663
Description: Rabbit polyclonal antibody to DDR2
Application: WB
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Sheep, Dog
Mol.Wt.: 97kDa,109kDa; 97kD(Calculated).
Uniprot: Q16832
RRID: AB_2839869

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(83%), Bovine(83%), Sheep(83%), Dog(83%)
Clonality:
Polyclonal
Specificity:
DDR2 Antibody detects endogenous levels of total DDR2.
RRID:
AB_2839869
Cite Format: Affinity Biosciences Cat# DF2663, RRID:AB_2839869.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

CD167 antigen-like family member B; CD167b; CD167b antigen; Cell migration inducing protein 20; DDR 2; DDR2; DDR2_HUMAN; Discoidin domain containing receptor 2; Discoidin domain receptor 2; Discoidin domain receptor family member 2; discoidin domain receptor tyrosine kinase 2; Discoidin domain-containing receptor 2; discoidin domain-containing receptor tyrosine kinase 2; Hydroxyaryl protein kinase; MIG20a; Migration inducing gene 16 protein; Neurotrophic tyrosine kinase; Neurotrophic tyrosine kinase receptor related 3; NTRKR 3; NTRKR3; Receptor protein tyrosine kinase TKT; Receptor protein-tyrosine kinase TKT; Receptor related 3; receptor-related 3; TKT; TYRO 10; TYRO10; Tyrosine kinase receptor related to neurotrophic TRK; Tyrosine protein kinase TYRO 10; Tyrosine protein kinase TYRO10; Tyrosine-protein kinase TYRO10; Tyrosylprotein kinase;

Immunogens

Immunogen:
Uniprot:

Q16832(DDR2_HUMAN) >>Visit HPA database.

Gene(ID):
DDR2(4921)
Expression:
Q16832 DDR2_HUMAN:

Detected in osteocytes, osteoblastic cells in subchondral bone, bone lining cells, tibia and cartilage (at protein level). Detected at high levels in heart and lung, and at low levels in brain, placenta, liver, skeletal muscle, pancreas, and kidney.

Description:
This tyrosine kinase receptor for fibrillar collagen mediates fibroblast migration and proliferation. Contributes to cutaneous wound healing.
Sequence:
MILIPRMLLVLFLLLPILSSAKAQVNPAICRYPLGMSGGQIPDEDITASSQWSESTAAKYGRLDSEEGDGAWCPEIPVEPDDLKEFLQIDLHTLHFITLVGTQGRHAGGHGIEFAPMYKINYSRDGTRWISWRNRHGKQVLDGNSNPYDIFLKDLEPPIVARFVRFIPVTDHSMNVCMRVELYGCVWLDGLVSYNAPAGQQFVLPGGSIIYLNDSVYDGAVGYSMTEGLGQLTDGVSGLDDFTQTHEYHVWPGYDYVGWRNESATNGYIEIMFEFDRIRNFTTMKVHCNNMFAKGVKIFKEVQCYFRSEASEWEPNAISFPLVLDDVNPSARFVTVPLHHRMASAIKCQYHFADTWMMFSEITFQSDAAMYNNSEALPTSPMAPTTYDPMLKVDDSNTRILIGCLVAIIFILLAIIVIILWRQFWQKMLEKASRRMLDDEMTVSLSLPSDSSMFNNNRSSSPSEQGSNSTYDRIFPLRPDYQEPSRLIRKLPEFAPGEEESGCSGVVKPVQPSGPEGVPHYAEADIVNLQGVTGGNTYSVPAVTMDLLSGKDVAVEEFPRKLLTFKEKLGEGQFGEVHLCEVEGMEKFKDKDFALDVSANQPVLVAVKMLRADANKNARNDFLKEIKIMSRLKDPNIIHLLAVCITDDPLCMITEYMENGDLNQFLSRHEPPNSSSSDVRTVSYTNLKFMATQIASGMKYLSSLNFVHRDLATRNCLVGKNYTIKIADFGMSRNLYSGDYYRIQGRAVLPIRWMSWESILLGKFTTASDVWAFGVTLWETFTFCQEQPYSQLSDEQVIENTGEFFRDQGRQTYLPQPAICPDSVYKLMLSCWRRDTKNRPSFQEIHLLLLQQGDE

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
83
Bovine
83
Sheep
83
Dog
83
Horse
75
Rabbit
75
Chicken
58
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q16832 As Substrate

Site PTM Type Enzyme
S37 Phosphorylation
S459 Phosphorylation
S461 Phosphorylation
S467 Phosphorylation
S469 Phosphorylation
T470 Phosphorylation
Y471 Phosphorylation P12931 (SRC)
R473 Methylation
Y481 Phosphorylation
S485 Phosphorylation
S501 Phosphorylation
Y521 Phosphorylation
S675 Phosphorylation
Y684 Phosphorylation
K720 Acetylation
Y736 Phosphorylation P12931 (SRC) , Q16832 (DDR2)
S737 Phosphorylation
Y740 Phosphorylation P12931 (SRC) , Q16832 (DDR2)
Y741 Phosphorylation P12931 (SRC) , Q16832 (DDR2)
Y813 Phosphorylation

PTMs - Q16832 As Enzyme

Substrate Site Source
Q16832 (DDR2) Y736 Uniprot
Q16832 (DDR2) Y740 Uniprot
Q16832 (DDR2) Y741 Uniprot

Research Backgrounds

Function:

Tyrosine kinase involved in the regulation of tissues remodeling. It functions as cell surface receptor for fibrillar collagen and regulates cell differentiation, remodeling of the extracellular matrix, cell migration and cell proliferation. Required for normal bone development. Regulates osteoblast differentiation and chondrocyte maturation via a signaling pathway that involves MAP kinases and leads to the activation of the transcription factor RUNX2. Regulates remodeling of the extracellular matrix by up-regulation of the collagenases MMP1, MMP2 and MMP13, and thereby facilitates cell migration and tumor cell invasion. Promotes fibroblast migration and proliferation, and thereby contributes to cutaneous wound healing.

PTMs:

N-glycosylated.

Tyrosine phosphorylated in response to collagen binding. Phosphorylated by SRC; this is required for activation and subsequent autophosphorylation on additional tyrosine residues.

Subcellular Location:

Cell membrane>Single-pass type I membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Detected in osteocytes, osteoblastic cells in subchondral bone, bone lining cells, tibia and cartilage (at protein level). Detected at high levels in heart and lung, and at low levels in brain, placenta, liver, skeletal muscle, pancreas, and kidney.

Subunit Structure:

Binds hydroxyproline-rich sequence motifs in fibrillar, glycosylated collagen, such as the GQOGVMGFO motif, where O stands for hydroxyproline. Interacts with SRC. Interacts (tyrosine phosphorylated) with SHC1.

Family&Domains:

Belongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily.

Restrictive clause

 

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