DYRK4 Antibody | Affinity Biosciences

WB
IHC
IF/ICC

1/3

DYRK4 Antibody - Western blot analysis of DYRK4 expression in NCI-H292 cell lysates ;developed using the ECL technique.

DYRK4 Antibody - DF2665 at 1/100 staining Mouse spleen tissue by IHC-P.

DYRK4 Antibody - DF2665 staining Hela cells by IF/ICC.

Product:	DYRK4 Antibody
Catalog:	DF2665
Description:	Rabbit polyclonal antibody to DYRK4
Application:	WB IHC IF/ICC
Reactivity:	Human, Mouse
Mol.Wt.:	60 kDa; 60kD(Calculated).
Uniprot:	Q9NR20
RRID:	AB_2839871

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse

Clonality:

Polyclonal

Specificity:

DYRK4 Antibody detects endogenous levels of total DYRK4.

RRID:

AB_2839871
Cite Format: Affinity Biosciences Cat# DF2665, RRID:AB_2839871.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Dual specificity tyrosine phosphorylation regulated kinase 4; Dual specificity tyrosine-phosphorylation-regulated kinase 4; Dyrk4; DYRK4_HUMAN; EC 2.7.12.1;

Immunogens

Immunogen:

Uniprot:

Q9NR20(DYRK4_HUMAN) >>Visit HPA database.

Gene(ID):

DYRK4(8798)

Description:

Possible non-essential role in spermiogenesis.;

Sequence:

Q9NR20 DYRK4_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MPASELKASEIPFHPSIKTQDPKAEEKSPKKQKVTLTAAEALKLFKNQLSPYEQSEILGYAELWFLGLEAKKLDTAPEKFSKTSFDDEHGFYLKVLHDHIAYRYEVLETIGKGSFGQVAKCLDHKNNELVALKIIRNKKRFHQQALMELKILEALRKKDKDNTYNVVHMKDFFYFRNHFCITFELLGINLYELMKNNNFQGFSLSIVRRFTLSVLKCLQMLSVEKIIHCDLKPENIVLYQKGQASVKVIDFGSSCYEHQKVYTYIQSRFYRSPEVILGHPYDVAIDMWSLGCITAELYTGYPLFPGENEVEQLACIMEVLGLPPAGFIQTASRRQTFFDSKGFPKNITNNRGKKRYPDSKDLTMVLKTYDTSFLDFLRRCLVWEPSLRMTPDQALKHAWIHQSRNLKPQPRPQTLRKSNSFFPSETRKDKVQGCHHSSRKADEITKETTEKTKDSPTKHVQHSGDQQDCLQHGADTVQLPQLVDAPKKSEAAVGAEVSMTSPGQSKNFSLKNTNVLPPIV

PTMs - Q9NR20 As Substrate

Q9NR20

Site	PTM Type	Enzyme	Source
T37	Phosphorylation		Uniprot
K71	Ubiquitination		Uniprot
K72	Ubiquitination		Uniprot
T211	Phosphorylation		Uniprot
S213	Phosphorylation		Uniprot
S222	Phosphorylation		Uniprot
Y262	Phosphorylation		Uniprot
T263	Phosphorylation		Uniprot
Y264	Phosphorylation	Q9NR20 (DYRK4)	Uniprot
S267	Phosphorylation		Uniprot
Y356	Phosphorylation		Uniprot
K360	Sumoylation		Uniprot
K417	Ubiquitination		Uniprot
S437	Phosphorylation		Uniprot
T445	Phosphorylation		Uniprot
T457	Phosphorylation		Uniprot

PTMs - Q9NR20 As Enzyme

Q9NR20

Substrate	Site	Source
Q9NR20 (DYRK4)	Y264	Uniprot

Research Backgrounds

Q9NR20_DYRK4_HUMAN

Function:

Possible non-essential role in spermiogenesis.

PTMs:

Autophosphorylation on Tyr-264 in the activation loop is required for kinase activity.

Subcellular Location:

Cytoplasm.

Cytoplasm. Nucleus.

Family&Domains:

Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MNB/DYRK subfamily.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.

DYRK4 Antibody - #DF2665