NUAK2 Antibody - #DF2671

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Product: NUAK2 Antibody
Catalog: DF2671
Description: Rabbit polyclonal antibody to NUAK2
Application: WB IHC
Reactivity: Human, Rat
Prediction: Pig, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 70 kDa; 70kD(Calculated).
Uniprot: Q9H093
RRID: AB_2839877

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 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Rat
Prediction:
Pig(89%), Horse(100%), Sheep(89%), Rabbit(100%), Dog(100%)
Clonality:
Polyclonal
Specificity:
NUAK2 Antibody detects endogenous levels of total NUAK2.
RRID:
AB_2839877
Cite Format: Affinity Biosciences Cat# DF2671, RRID:AB_2839877.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

DKFZp434J037; DKFZp686F01113; FLJ90349; NUAK family SNF1 like kinase 2; NUAK family SNF1-like kinase 2; Nuak2; NUAK2_HUMAN; Omphalocele kinase 2; SNARK; SNF1/AMP activated protein kinase; SNF1/AMP kinase related kinase; SNF1/AMP kinase-related kinase;

Immunogens

Immunogen:
Uniprot:

Q9H093(NUAK2_HUMAN) >>Visit HPA database.

Gene(ID):
NUAK2(81788)
Description:
Stress-activated kinase involved in tolerance to glucose starvation. Induces cell-cell detachment by increasing F-actin conversion to G-actin. Expression is induced by CD95 or TNF-alpha, via NF-kappa-B. Protects cells from CD95-mediated apoptosis and is required for the increased motility and invasiveness of CD95-activated tumor cells.;
Sequence:
MESLVFARRSGPTPSAAELARPLAEGLIKSPKPLMKKQAVKRHHHKHNLRHRYEFLETLGKGTYGKVKKARESSGRLVAIKSIRKDKIKDEQDLMHIRREIEIMSSLNHPHIIAIHEVFENSSKIVIVMEYASRGDLYDYISERQQLSEREARHFFRQIVSAVHYCHQNRVVHRDLKLENILLDANGNIKIADFGLSNLYHQGKFLQTFCGSPLYASPEIVNGKPYTGPEVDSWSLGVLLYILVHGTMPFDGHDHKILVKQISNGAYREPPKPSDACGLIRWLLMVNPTRRATLEDVASHWWVNWGYATRVGEQEAPHEGGHPGSDSARASMADWLRRSSRPLLENGAKVCSFFKQHAPGGGSTTPGLERQHSLKKSRKENDMAQSLHSDTADDTAHRPGKSNLKLPKGILKKKVSASAEGVQEDPPELSPIPASPGQAAPLLPKKGILKKPRQRESGYYSSPEPSESGELLDAGDVFVSGDPKEQKPPQASGLLLHRKGILKLNGKFSQTALELAAPTTFGSLDELAPPRPLARASRPSGAVSEDSILSSESFDQLDLPERLPEPPLRGCVSVDNLTGLEEPPSEGPGSCLRRWRQDPLGDSCFSLTDCQEVTATYRQALRVCSKLT

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Dog
100
Rabbit
100
Pig
89
Sheep
89
Zebrafish
78
Chicken
78
Xenopus
67
Bovine
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9H093 As Substrate

Site PTM Type Enzyme
M1 Acetylation
S30 Phosphorylation
T58 Phosphorylation
K81 Ubiquitination
Y138 Phosphorylation
Y140 Phosphorylation
S142 Phosphorylation
T208 Phosphorylation Q15831 (STK11)
Y267 Phosphorylation
S435 Phosphorylation
S462 Phosphorylation
K487 Ubiquitination
S523 Phosphorylation
S544 Phosphorylation
S547 Phosphorylation
S553 Phosphorylation

PTMs - Q9H093 As Enzyme

Substrate Site Source
O95835 (LATS1) S464 Uniprot

Research Backgrounds

Function:

Stress-activated kinase involved in tolerance to glucose starvation. Induces cell-cell detachment by increasing F-actin conversion to G-actin. Expression is induced by CD95 or TNF-alpha, via NF-kappa-B. Protects cells from CD95-mediated apoptosis and is required for the increased motility and invasiveness of CD95-activated tumor cells. Able to phosphorylate 'Ser-464' of LATS1.

PTMs:

Phosphorylated at Thr-208 by STK11/LKB1 in complex with STE20-related adapter-alpha (STRADA) pseudo kinase and CAB39. Autophosphorylated in vitro.

Family&Domains:

Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily.

References

1). Upregulation of NUAK2: A novel prognostic marker in breast cancer. HISTOLOGY AND HISTOPATHOLOGY, 2022 (PubMed: 36440828) [IF=2.5]

Application: WB    Species: Human    Sample: breast cancer tissues and cell

Fig. 1. Expression of NUAK2 is elevated in breast cancer tissues and cell lines. A. RT-qPCR was performed to determine the expression of NUAK2 in the breast cancer and normal tissues. B. Western blotting assays were performed to assess the protein levels of NUAK2 in 10 breast cancer tissues with paired adjacent non-tumoral tissues. C. Western blotting analysis of NUAK2 protein expression in breast cancer cell lines and primary cultured human mammary epithelial cell (HEMC).

Application: IHC    Species: Human    Sample: breast cancer tissues and cell

Fig. 2. Immunochemistry analyses of NUAK2 expression in breast cancer tissue samples. Representative images of negative staining of NUAK2 (A), weakly positive staining (+) of NUAK2 (B), positive staining (++) of NUAK2 (C) and strongly positive staining (+++) of NUAK2 (D). x 400.

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Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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