PCTK1 Antibody - #DF2672
| Product: | PCTK1 Antibody |
| Catalog: | DF2672 |
| Description: | Rabbit polyclonal antibody to PCTK1 |
| Application: | WB |
| Reactivity: | Human, Mouse, Rat |
| Prediction: | Pig, Bovine, Horse, Sheep, Rabbit, Dog |
| Mol.Wt.: | 56 kDa; 56kD(Calculated). |
| Uniprot: | Q00536 |
| RRID: | AB_2839878 |
Related Downloads
Protocols
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# DF2672, RRID:AB_2839878.
Fold/Unfold
Cdk16; CDK16_HUMAN; Cell division protein kinase 16; Cyclin-dependent kinase 16; PCTAIRE; PCTAIRE protein kinase 1; PCTAIRE-motif protein kinase 1; PCTAIRE1; PCTGAIRE; PCTK1; Serine/threonine-protein kinase PCTAIRE-1;
Immunogens
- Q00536 CDK16_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MDRMKKIKRQLSMTLRGGRGIDKTNGAPEQIGLDESGGGGGSDPGEAPTRAAPGELRSARGPLSSAPEIVHEDLKMGSDGESDQASATSSDEVQSPVRVRMRNHPPRKISTEDINKRLSLPADIRLPEGYLEKLTLNSPIFDKPLSRRLRRVSLSEIGFGKLETYIKLDKLGEGTYATVYKGKSKLTDNLVALKEIRLEHEEGAPCTAIREVSLLKDLKHANIVTLHDIIHTEKSLTLVFEYLDKDLKQYLDDCGNIINMHNVKLFLFQLLRGLAYCHRQKVLHRDLKPQNLLINERGELKLADFGLARAKSIPTKTYSNEVVTLWYRPPDILLGSTDYSTQIDMWGVGCIFYEMATGRPLFPGSTVEEQLHFIFRILGTPTEETWPGILSNEEFKTYNYPKYRAEALLSHAPRLDSDGADLLTKLLQFEGRNRISAEDAMKHPFFLSLGERIHKLPDTTSIFALKEIQLQKEASLRSSSMPDSGRPAFRVVDTEF
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q00536 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S12 | Phosphorylation | Q8IWQ3 (BRSK2) , P17612 (PRKACA) | Uniprot |
| T14 | Phosphorylation | Uniprot | |
| R19 | Methylation | Uniprot | |
| S36 | Phosphorylation | Uniprot | |
| S42 | Phosphorylation | Uniprot | |
| S64 | Phosphorylation | Uniprot | |
| S65 | Phosphorylation | Uniprot | |
| S78 | Phosphorylation | Uniprot | |
| S82 | Phosphorylation | Uniprot | |
| S86 | Phosphorylation | Uniprot | |
| T88 | Phosphorylation | Uniprot | |
| S89 | Phosphorylation | Uniprot | |
| S90 | Phosphorylation | Uniprot | |
| S95 | Phosphorylation | Uniprot | |
| K108 | Acetylation | Uniprot | |
| S110 | Phosphorylation | P17612 (PRKACA) | Uniprot |
| T111 | Phosphorylation | Uniprot | |
| K116 | Acetylation | Uniprot | |
| K116 | Ubiquitination | Uniprot | |
| S119 | Phosphorylation | P17612 (PRKACA) | Uniprot |
| K133 | Ubiquitination | Uniprot | |
| T135 | Phosphorylation | Uniprot | |
| S138 | Phosphorylation | P24941 (CDK2) | Uniprot |
| K143 | Ubiquitination | Uniprot | |
| S146 | Phosphorylation | Uniprot | |
| S153 | Phosphorylation | P17612 (PRKACA) | Uniprot |
| S155 | Phosphorylation | Uniprot | |
| T164 | Phosphorylation | Uniprot | |
| K167 | Ubiquitination | Uniprot | |
| T175 | Phosphorylation | Uniprot | |
| Y176 | Phosphorylation | Uniprot | |
| T178 | Phosphorylation | Uniprot | |
| Y180 | Phosphorylation | Uniprot | |
| K194 | Ubiquitination | Uniprot | |
| K216 | Ubiquitination | Uniprot | |
| K288 | Ubiquitination | Uniprot | |
| K301 | Ubiquitination | Uniprot | |
| S319 | Phosphorylation | Uniprot | |
| T380 | Phosphorylation | Uniprot | |
| S391 | Phosphorylation | Uniprot | |
| K425 | Ubiquitination | Uniprot | |
| K455 | Ubiquitination | Uniprot | |
| K466 | Ubiquitination | Uniprot | |
| K472 | Ubiquitination | Uniprot | |
| S478 | Phosphorylation | Uniprot | |
| S479 | Phosphorylation | Uniprot | |
| S480 | Phosphorylation | Uniprot |
PTMs - Q00536 As Enzyme
Research Backgrounds
Protein kinase that plays a role in vesicle-mediated transport processes and exocytosis. Regulates GH1 release by brain neurons. Phosphorylates NSF, and thereby regulates NSF oligomerization. Required for normal spermatogenesis. Regulates neuron differentiation and dendrite development (By similarity). Plays a role in the regulation of insulin secretion in response to changes in blood glucose levels. Can phosphorylate CCNY at 'Ser-336' (in vitro).
Phosphorylation at Ser-153 inhibits kinase activity.
Cytoplasm. Cytoplasmic vesicle>Secretory vesicle. Cell membrane>Peripheral membrane protein>Cytoplasmic side. Cell junction>Synapse>Synaptosome.
Note: Colocalizes with insulin in pancreas islets. Recruited to the cell membrane by CCNY.
Detected in pancreas islets (at protein level). Detected in brain and pancreas.
Found in a complex containing CABLES1, CDK17 and TDRD7. Interacts with YWHAH, YWHAQ and YWHAZ. Interacts with NSF. Identified in a complex with NSF, syntaxin-1, synaptotagmin, SYN1, SYP and CDK5R1 (By similarity). Interacts with BRSK2. Interacts with CCNY; this increases the CDK16 kinase activity.
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
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