Product: TYRO3 Antibody
Catalog: DF2680
Description: Rabbit polyclonal antibody to TYRO3
Application: WB
Cited expt.: WB
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 97 kDa ,; 97kD(Calculated).
Uniprot: Q06418
RRID: AB_2839886

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Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%)
Clonality:
Polyclonal
Specificity:
TYRO3 Antibody detects endogenous levels of total TYRO3.
RRID:
AB_2839886
Cite Format: Affinity Biosciences Cat# DF2680, RRID:AB_2839886.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Brt; BYK; DTK; Etk-2; protein tyrosine kinase 3; Rek; RSE; SKY; Tif; tyro3; TYRO3 protein tyrosine kinase; TYRO3_HUMAN; Tyrosine-protein kinase byk; Tyrosine-protein kinase DTK; Tyrosine-protein kinase receptor TYRO3; Tyrosine-protein kinase RSE; Tyrosine-protein kinase SKY; Tyrosine-protein kinase TIF;

Immunogens

Immunogen:

A synthesized peptide derived from human TYRO3, corresponding to a region within C-terminal amino acids.

Uniprot:
Gene(ID):
Expression:
Q06418 TYRO3_HUMAN:

Abundant in the brain and lower levels in other tissues.

Sequence:
MALRRSMGRPGLPPLPLPPPPRLGLLLAALASLLLPESAAAGLKLMGAPVKLTVSQGQPVKLNCSVEGMEEPDIQWVKDGAVVQNLDQLYIPVSEQHWIGFLSLKSVERSDAGRYWCQVEDGGETEISQPVWLTVEGVPFFTVEPKDLAVPPNAPFQLSCEAVGPPEPVTIVWWRGTTKIGGPAPSPSVLNVTGVTQSTMFSCEAHNLKGLASSRTATVHLQALPAAPFNITVTKLSSSNASVAWMPGADGRALLQSCTVQVTQAPGGWEVLAVVVPVPPFTCLLRDLVPATNYSLRVRCANALGPSPYADWVPFQTKGLAPASAPQNLHAIRTDSGLILEWEEVIPEAPLEGPLGPYKLSWVQDNGTQDELTVEGTRANLTGWDPQKDLIVRVCVSNAVGCGPWSQPLVVSSHDRAGQQGPPHSRTSWVPVVLGVLTALVTAAALALILLRKRRKETRFGQAFDSVMARGEPAVHFRAARSFNRERPERIEATLDSLGISDELKEKLEDVLIPEQQFTLGRMLGKGEFGSVREAQLKQEDGSFVKVAVKMLKADIIASSDIEEFLREAACMKEFDHPHVAKLVGVSLRSRAKGRLPIPMVILPFMKHGDLHAFLLASRIGENPFNLPLQTLIRFMVDIACGMEYLSSRNFIHRDLAARNCMLAEDMTVCVADFGLSRKIYSGDYYRQGCASKLPVKWLALESLADNLYTVQSDVWAFGVTMWEIMTRGQTPYAGIENAEIYNYLIGGNRLKQPPECMEDVYDLMYQCWSADPKQRPSFTCLRMELENILGQLSVLSASQDPLYINIERAEEPTAGGSLELPGRDQPYSGAGDGSGMGAVGGTPSDCRYILTPGGLAEQPGQAEHQPESPLNETQRLLLLQQGLLPHSSC

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Xenopus
71
Chicken
71
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

Research Backgrounds

Function:

Receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding to several ligands including TULP1 or GAS6. Regulates many physiological processes including cell survival, migration and differentiation. Ligand binding at the cell surface induces dimerization and autophosphorylation of TYRO3 on its intracellular domain that provides docking sites for downstream signaling molecules. Following activation by ligand, interacts with PIK3R1 and thereby enhances PI3-kinase activity. Activates the AKT survival pathway, including nuclear translocation of NF-kappa-B and up-regulation of transcription of NF-kappa-B-regulated genes. TYRO3 signaling plays a role in various processes such as neuron protection from excitotoxic injury, platelet aggregation and cytoskeleton reorganization. Plays also an important role in inhibition of Toll-like receptors (TLRs)-mediated innate immune response by activating STAT1, which selectively induces production of suppressors of cytokine signaling SOCS1 and SOCS3.

(Microbial infection) Acts as a receptor for lassa virus and lymphocytic choriomeningitis virus, possibly through GAS6 binding to phosphatidyl-serine at the surface of virion envelope.

(Microbial infection) Acts as a receptor for Ebolavirus, possibly through GAS6 binding to phosphatidyl-serine at the surface of virion envelope.

PTMs:

Autophosphorylated.

Subcellular Location:

Cell membrane>Single-pass type I membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Abundant in the brain and lower levels in other tissues.

Family&Domains:

Belongs to the protein kinase superfamily. Tyr protein kinase family. AXL/UFO subfamily.

References

1). Screen of FDA-approved drug library identifies vitamin K as anti-ferroptotic drug for osteoarthritis therapy through Gas6. Journal of Pharmaceutical Analysis, 2025 [IF=6.1]

Application: WB    Species: Mouse    Sample:

Fig. 6. AXL receptor tyrosine kinase (AXL)/phosphatidylinositol 3-kinase (PI3K)/AKT serine/threonine kinase (AKT) pathway mediates the anti-ferroptotic effects of growth arrest-specific 6 (Gas6) and vitamin K. (A) Evaluation of anti-ferroptotic effect of Gas6 in chondrocytes. Chondrocytes were pretreated with indicated Gas6 concentrations for 2 h followed by treated with RSL3 (0.5 μM) for 24 h. (B) The protein expression levels of TAM receptors (AXL, TYRO3) in chondrocytes were detected by Western blot analysis. Chondrocytes were treated with RSL3 (0.5 μM, 24 h), vitamin K1(VK1, 10 μM, 24 h) or Gas6 (100 ng/mL, 2 h). (C) Cell viability was detected by Cell Counting Kit-8 (CCK-8). Chondrocytes were transfected with AXL small interfering RNA (si-AXL, #1, #3), TYRO3 small interfering RNA (si-TYRO3, #2, #3) or negative control siRNA (si-NC). A total of 24 h after transfection, chondrocytes were pretreated with Gas6 (100 ng/mL) for 2 h followed by treatment with RSL3 (0.5 μM) for 24 h. (D, E) Intracellular malondialdehyde (MDA) analysis (D) and reduced glutathione/oxidized glutathione (GSH/GSSG) (E) ratio of chondrocytes. Chondrocytes were transfected with si-AXL #1 or si-NC for 24 h. A total of 24 h after transfection, chondrocytes were pretreated with Gas6 (100 ng/mL) for 2 h followed by treatment with RSL3 (0.5 μM) for 24 h. (F) Intracellular ferrous levels detected by FerroOrange probe and lipid peroxidation evaluated by BODIPY 581/591 C11 staining of chondrocytes (green: oxidized lipids; red: lipids; and blue: Hoechst). (G) The mRNA levels of Mmp13, Adamts4, Col2a1 and Acan in chondrocytes were evaluated by reverse transcription-quantitative polymerase chain reaction (RT-qPCR). (H) The protein expression levels of matrix metalloproteinase 13 (MMP13), ADAMTS4, collagen type II (COL II) and Aggrecan in chondrocytes were detected by Western blot analysis. (I) The phosphorylation of AXL (P-AXL) was detected by Western blot analysis. After pretreated with R428 (AXL inhibitor, 5 μM) for 15 min, chondrocytes were treated with Gas6 (100 ng/mL) for 2 h followed by treatment with RSL3 (0.5 μM) for 2 h. (J) Cell viability were detected by CCK-8. Chondrocytes were pretreated with R428 (5 μM) for 15 min followed by treated with Gas6 (100 ng/mL, 2 h) and RSL3 (0.5 μM, 24 h). (K) The P-AKT was detected by Western blot analysis. After pretreated with GDC-0941 (PI3K inhibitor, 5 μM) for 15 min, chondrocytes were treated with Gas6 (100 ng/mL) for 2 h followed by treatment with RSL3 (0.5 μM) for 2 h. (L) Cell viability were detected by CCK-8. Chondrocytes were pretreated with GDC-0941 (5 μM) for 15 min followed by treatment with Gas6 (100 ng/mL, 2 h) and RSL3 (0.5 μM, 24 h). (M) Cell viability was detected by CCK-8. Chondrocytes were transfected with si-AXL, si-TYRO3 or si-NC. A total of 24 h after transfection, chondrocytes were treated with VK1 (10 μM) and RSL3 (0.5 μM) for 24 h. (N) Cell viability were detected by CCK-8. Chondrocytes were pretreated with R428 (5 μM) or GDC-0941 (5 μM) for 15 min followed by treated with VK1 (10 μM) and RSL3 (0.5 μM) for 24 h. (O) Graphic abstract of our study indicating how vitamin K and Gas6 protects against osteoarthritis through inhibiting ferroptosis. Data are the mean ± standard deviation (SD) from three independent experiments with one-way analysis of variance (ANOVA) with Dunnett's post hoc test. ∗∗P < 0.01; ns: not significant. PBS: phosphate buffered saline;Ctrl: Control.

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