SRMS Antibody | Affinity Biosciences

WB
IHC
IF/ICC

1/4

SRMS Antibody - Western blot analysis of extracts from MCF7 cells(serum starvation treatment), using SRMS Antibody.

SRMS Antibody - DF2682 at 1/100 staining mouse liver tissue by IHC-P.

SRMS Antibody - DF2682 at 1/100 staining rat muscle tissue by IHC-P.

SRMS Antibody - DF2682 staining Hela cells by IF/ICC.

Product:	SRMS Antibody
Catalog:	DF2682
Description:	Rabbit polyclonal antibody to SRMS
Application:	WB IHC IF/ICC
Reactivity:	Human, Rat
Prediction:	Pig, Bovine, Horse, Sheep
Mol.Wt.:	65 kDa,; 55kD(Calculated).
Uniprot:	Q9H3Y6
RRID:	AB_2839888

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

IF/ICC 1:100-1:500, WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Rat

Prediction:

Pig(88%), Bovine(88%), Horse(88%), Sheep(88%)

Clonality:

Polyclonal

Specificity:

SRMS Antibody detects endogenous levels of total SRMS.

RRID:

AB_2839888
Cite Format: Affinity Biosciences Cat# DF2682, RRID:AB_2839888.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

C20orf148; dJ697K14.1; EC 2.7.1.112; Src related kinase lacking C terminal regulatory tyrosine and N terminal myristylation sites; SRM; SRMS; SRMS_HUMAN; Tyrosine protein kinase Srms; Tyrosine-protein kinase Srms;

Immunogens

Immunogen:

Uniprot:

Q9H3Y6(SRMS_HUMAN) >>Visit HPA database.

Gene(ID):

SRMS(6725)

Expression:

Q9H3Y6 SRMS_HUMAN:

Highly expressed in most breast cancers (at protein level).

Description:

May be involved in proliferation or differentiation of keratinocytes in the skin.

Sequence:

Q9H3Y6 SRMS_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MEPFLRRRLAFLSFFWDKIWPAGGEPDHGTPGSLDPNTDPVPTLPAEPCSPFPQLFLALYDFTARCGGELSVRRGDRLCALEEGGGYIFARRLSGQPSAGLVPITHVAKASPETLSDQPWYFSGVSRTQAQQLLLSPPNEPGAFLIRPSESSLGGYSLSVRAQAKVCHYRVSMAADGSLYLQKGRLFPGLEELLTYYKANWKLIQNPLLQPCMPQKAPRQDVWERPHSEFALGRKLGEGYFGEVWEGLWLGSLPVAIKVIKSANMKLTDLAKEIQTLKGLRHERLIRLHAVCSGGEPVYIVTELMRKGNLQAFLGTPEGRALRLPPLLGFACQVAEGMSYLEEQRVVHRDLAARNVLVDDGLACKVADFGLARLLKDDIYSPSSSSKIPVKWTAPEAANYRVFSQKSDVWSFGVLLHEVFTYGQCPYEGMTNHETLQQIMRGYRLPRPAACPAEVYVLMLECWRSSPEERPSFATLREKLHAIHRCHP

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

Horse

Bovine

Sheep

Dog

Xenopus

Zebrafish

Chicken

Rabbit

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9H3Y6 As Substrate

Q9H3Y6

Site	PTM Type	Enzyme	Source
S94	Phosphorylation		Uniprot
Y380	Phosphorylation	Q9H3Y6 (SRMS)	Uniprot

PTMs - Q9H3Y6 As Enzyme

Q9H3Y6

Substrate	Site	Source
Q13882 (PTK6)	Y447	Uniprot
Q9H3Y6 (SRMS)	Y380	Uniprot

Research Backgrounds

Q9H3Y6_SRMS_HUMAN

Function:

Non-receptor tyrosine-protein kinase which phosphorylates DOK1 on tyrosine residues. Also phosphorylates KHDRBS1/SAM68 and VIM on tyrosine residues. Phosphorylation of KHDRBS1 is EGF-dependent.

Subcellular Location:

Cytoplasm.
Note: Localizes to punctate cytoplasmic structures.

Tissue Specificity:

Highly expressed in most breast cancers (at protein level).

Subunit Structure:

Interacts (via the SH2 and SH3 domains) with DOK1. Interacts with KHDRBS1/SAM68 and VIM.

Family&Domains:

The N-terminal region regulates its kinase activity.

Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.

SRMS Antibody - #DF2682