GPR55 Antibody - #DF2752

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Product: GPR55 Antibody
Catalog: DF2752
Description: Rabbit polyclonal antibody to GPR55
Application: WB IHC
Reactivity: Human
Prediction: Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 37kDa; 37kD(Calculated).
Uniprot: Q9Y2T6
RRID: AB_2839958

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Prediction:
Bovine(80%), Horse(89%), Sheep(90%), Rabbit(90%), Dog(80%)
Clonality:
Polyclonal
Specificity:
GPR55 Antibody detects endogenous levels of total GPR55.
RRID:
AB_2839958
Cite Format: Affinity Biosciences Cat# DF2752, RRID:AB_2839958.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

G protein coupled receptor 55; G-protein coupled receptor 55; GPCR GPR55; GPR 55; Gpr55; GPR55_HUMAN; LPIR1; Probable G protein coupled receptor 55;

Immunogens

Immunogen:
Uniprot:

Q9Y2T6(GPR55_HUMAN) >>Visit HPA database.

Gene(ID):
GPR55(9290)
Expression:
Q9Y2T6 GPR55_HUMAN:

Expressed in the caudate nucleus and putamen, but not detected in the hippocampus, thalamus, pons cerebellum, frontal cortex of the brain or in the liver. Expressed in osteoclasts and osteoblasts.

Description:
May be involved in hyperalgesia associated with inflammatory and neuropathic pain (By similarity). Receptor for L-alpha-lysophosphatidylinositol (LPI). LPI induces Ca(2+) release from intracellular stores via the heterotrimeric G protein GNA13 and RHOA. Putative cannabinoid receptor. May play a role in bone physiology by regulating osteoclast number and function.
Sequence:
MSQQNTSGDCLFDGVNELMKTLQFAVHIPTFVLGLLLNLLAIHGFSTFLKNRWPDYAATSIYMINLAVFDLLLVLSLPFKMVLSQVQSPFPSLCTLVECLYFVSMYGSVFTICFISMDRFLAIRYPLLVSHLRSPRKIFGICCTIWVLVWTGSIPIYSFHGKVEKYMCFHNMSDDTWSAKVFFPLEVFGFLLPMGIMGFCCSRSIHILLGRRDHTQDWVQQKACIYSIAASLAVFVVSFLPVHLGFFLQFLVRNSFIVECRAKQSISFFLQLSMCFSNVNCCLDVFCYYFVIKEFRMNIRAHRPSRVQLVLQDTTISRG

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Rabbit
90
Sheep
90
Horse
89
Bovine
80
Dog
80
Chicken
67
Pig
0
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

Research Backgrounds

Function:

May be involved in hyperalgesia associated with inflammatory and neuropathic pain (By similarity). Receptor for L-alpha-lysophosphatidylinositol (LPI). LPI induces Ca(2+) release from intracellular stores via the heterotrimeric G protein GNA13 and RHOA. Putative cannabinoid receptor. May play a role in bone physiology by regulating osteoclast number and function.

Subcellular Location:

Cell membrane>Multi-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in the caudate nucleus and putamen, but not detected in the hippocampus, thalamus, pons cerebellum, frontal cortex of the brain or in the liver. Expressed in osteoclasts and osteoblasts.

Family&Domains:

Belongs to the G-protein coupled receptor 1 family.

References

1). The GPR55 antagonist CID16020046 mitigates advanced glycation end products (AGEs)-induced chondrocyte activation. CHEMICO-BIOLOGICAL INTERACTIONS, 2020 (PubMed: 32360554) [IF=4.7]

Application: WB    Species: human    Sample: ATDC5 cells

Fig. 1. |Advanced Glycation End Products(AGEs) increased the expression of GPR55 in human ATDC5 cells. Cells were stimulated with 50, 100, and 200 μg/ml AGEs for 24 h. (A). Real time PCR analysis revealed that AGEs increased the expression of GPR55 at the mRNA levels; (B). Western blot analysis revealed that AGEs increased the expression of GPR55 at the protein levels (*, **, ***, P < 0.05, 0.01, 0.005 vs.previous group, n = 4).

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