F4/80 Antibody - #DF2789
(36)
(17)
Product Info
Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Reactivity:
Human,Rat
Prediction:
Mouse(%), Bovine(83%), Sheep(83%)
Clonality:
Polyclonal
Specificity:
F4/80 Antibody detects endogenous levels of total F4/80.
RRID:
AB_2839995
Cite Format: Affinity Biosciences Cat# DF2789, RRID:AB_2839995.
Cite Format: Affinity Biosciences Cat# DF2789, RRID:AB_2839995.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:
Fold/Unfold
ADGRE1; Adhesion G protein coupled receptor E1; Adhesion G protein-coupled receptor E1; AGRE1_HUMAN; Cell surface glycoprotein EMR1; Cell surface glycoprotein F4/80; DD7A5 7; Egf like module containing mucin like hormone receptor like 1; Egf like module containing mucin like hormone receptor like sequence 1; EGF like module receptor 1; EGF TM7; EGF-like module receptor 1; EGF-like module-containing mucin-like hormone receptor-like 1; EGFTM7; EMR 1; EMR1 hormone receptor; Gpf480; Ly71; Lymphocyte antigen 71; TM7LN3;
Immunogens
Immunogen:
A synthesized peptide derived from human F4/80, corresponding to a region within C-terminal amino acids.
Uniprot:
Gene(ID):
Expression:
Description:
Could be involved in cell-cell interactions;
Sequence:
- Q14246 AGRE1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MRGFNLLLFWGCCVMHSWEGHIRPTRKPNTKGNNCRDSTLCPAYATCTNTVDSYYCACKQGFLSSNGQNHFKDPGVRCKDIDECSQSPQPCGPNSSCKNLSGRYKCSCLDGFSSPTGNDWVPGKPGNFSCTDINECLTSSVCPEHSDCVNSMGSYSCSCQVGFISRNSTCEDVDECADPRACPEHATCNNTVGNYSCFCNPGFESSSGHLSFQGLKASCEDIDECTEMCPINSTCTNTPGSYFCTCHPGFAPSNGQLNFTDQGVECRDIDECRQDPSTCGPNSICTNALGSYSCGCIAGFHPNPEGSQKDGNFSCQRVLFKCKEDVIPDNKQIQQCQEGTAVKPAYVSFCAQINNIFSVLDKVCENKTTVVSLKNTTESFVPVLKQISTWTKFTKEETSSLATVFLESVESMTLASFWKPSANITPAVRTEYLDIESKVINKECSEENVTLDLVAKGDKMKIGCSTIEESESTETTGVAFVSFVGMESVLNERFFKDHQAPLTTSEIKLKMNSRVVGGIMTGEKKDGFSDPIIYTLENIQPKQKFERPICVSWSTDVKGGRWTSFGCVILEASETYTICSCNQMANLAVIMASGELTMDFSLYIISHVGIIISLVCLVLAIATFLLCRSIRNHNTYLHLHLCVCLLLAKTLFLAGIHKTDNKMGCAIIAGFLHYLFLACFFWMLVEAVILFLMVRNLKVVNYFSSRNIKMLHICAFGYGLPMLVVVISASVQPQGYGMHNRCWLNTETGFIWSFLGPVCTVIVINSLLLTWTLWILRQRLSSVNAEVSTLKDTRLLTFKAFAQLFILGCSWVLGIFQIGPVAGVMAYLFTIINSLQGAFIFLIHCLLNGQVREEYKRWITGKTKPSSQSQTSRILLSSMPSASKTG
Predictions
Predictions:
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
Species
Results
Score
Bovine
83
Sheep
83
Dog
67
Pig
0
Horse
0
Xenopus
0
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence
High(score>80) Medium(80>score>50) Low(score<50) No confidence
Research Backgrounds
Function:
Orphan receptor involved in cell adhesion and probably in cell-cell interactions specifically involving cells of the immune system. May play a role in regulatory T-cells (Treg) development.
Subcellular Location:
Cell membrane>Multi-pass membrane protein.
Tissue Specificity:
Expression is restricted to eosinophils.
Family&Domains:
Belongs to the G-protein coupled receptor 2 family. Adhesion G-protein coupled receptor (ADGR) subfamily.
References
1). Interorgan communication in neurogenic heterotopic ossification: the role of brain-derived extracellular vesicles. Bone research, 2024
(PubMed: 38383487)
[IF=14.3]
2). Interplay between lipid dysregulation and ferroptosis in chondrocytes and the targeted therapy effect of metformin on osteoarthritis. Journal of advanced research, 2025
(PubMed: 38621621)
[IF=11.4]
3). Endothelial cell-derived MMP19 promotes pulmonary fibrosis by inducing E(nd)MT and monocyte infiltration. Cell communication and signaling : CCS, 2023
(PubMed: 36915092)
[IF=8.4]
4). Zwitterionic hydrogel-coated heart valves with improved endothelialization and anti-calcification properties. Materials science & engineering-C, Materials for biological applications, 2021
(PubMed: 34474880)
[IF=8.1]
5). Hepatic TGFβr1 Deficiency Attenuates Lipopolysaccharide/D-Galactosamine–Induced Acute Liver Failure Through Inhibiting GSK3β-Nrf2-Mediated Hepatocyte Apoptosis and Ferroptosis. Cellular and Molecular Gastroenterology and Hepatology, 2022
(PubMed: 35202887)
[IF=7.1]
Application: IHC Species: Mice Sample:
Figure 1
LPS/D-GalN-induced ALF enhances apoptosis and ferroptosis. (A) The survival rate of WT mice treated with vehicle or LPS/D-GalN (LPS, 20 μg/kg; D-GalN, 700 mg/kg) (n = 10). (B) Representative pictures of WT mice treated with vehicle or LPS/D-GalN for 3, 5, and 6 hours (n = 10). (C) Serum levels of ALT and AST with or without LPS/D-GalN co-injection (n = 6). (D) H&E and TUNEL staining after LPS/D-GalN co-injection (n = 6). (E) Immunohistochemistry of TNFα and F4/80 staining after LPS/D-GalN co-injection (n = 6). (F) Transmission electron microscope of liver tissues (n = 3). (G) MDA assay of liver homogenates (n = 5). (H and J) Western blot analyses of TFR, DMT1, GPX4, and XCT from WT mice (H) with or without LPS/D-GalN and (I) quantitative results (n = 3–9). (I and K) FSP1, DHODH, and POR proteins. Western blot analyses of WT mice (I) with or without LPS/D-GalN and (K) quantitative results (n = 3–9). (L) Immunohistochemistry of DMT1 staining and immunofluorescence of Ptgs2 in livers with or without LPS/D-GalN (n = 6). (M) Quantitative results of DMT1 and Ptgs2. All data were obtained from WT mice. Scale bars: 100 μm. Data are presented as means ± SEM. ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001, ∗∗∗∗P < 0.0001. DAPI, 4′,6-diamidino-2-phenylindole; GAPDH, glyceraldehyde-3-phosphate dehydrogenase.
6). Insights into the mechanism of action of pterostilbene against influenza A virus-induced acute lung injury. Phytomedicine : international journal of phytotherapy and phytopharmacology, 2024
(PubMed: 38583346)
[IF=6.7]
7). Xuanfei Baidu formula alleviates impaired mitochondrial dynamics and activated NLRP3 inflammasome by repressing NF-κB and MAPK pathways in LPS-induced ALI and inflammation models. Phytomedicine, 2023
(PubMed: 36423572)
[IF=6.7]
8). Targeted inhibition of CX3CL1 limits podocytes ferroptosis to ameliorate cisplatin-induced acute kidney injury. Molecular medicine (Cambridge, Mass.), 2023
(PubMed: 37875838)
[IF=6.0]
Application: WB Species: Mouse Sample: kidney tissues
Fig. 5 CX3CL1 deficiency attenuated kidney inflammation and macrophage infiltration in cisplatin-induced model mice. A Western blotting was conducted to assess the expression profiles of IL-6, TNF-α, and F4/80 in kidney tissues. B, C Levels of IL-6 and TNF-α in mouse sera. D Image representations of TNF-α and F4/80 expression by immunohistochemistry. Scale bar = 20 μm. E podocytes were transfected with siRNA-CX3CL1 as indicated and treated with cisplatin (10 μM) for 24 h. CX3CL1, TNF-α, and IL-6 expression profiles were then measured by western blotting. F, G Levels of IL-6 and TNF-α in podocyte cell supernatant. H, I Representative micrographs of CX3CL1 and TNF-α staining in podocytes. Scale bar = 10 μm. (IL-6, interleukin 6; TNF-α, tumor necrosis factor-α; P value was calculated by one-way analysis of variance and Tukey’s test.
Application: IHC Species: Mouse Sample: kidney tissues
Fig. 5 CX3CL1 deficiency attenuated kidney inflammation and macrophage infiltration in cisplatin-induced model mice. A Western blotting was conducted to assess the expression profiles of IL-6, TNF-α, and F4/80 in kidney tissues. B, C Levels of IL-6 and TNF-α in mouse sera. D Image representations of TNF-α and F4/80 expression by immunohistochemistry. Scale bar = 20 μm. E podocytes were transfected with siRNA-CX3CL1 as indicated and treated with cisplatin (10 μM) for 24 h. CX3CL1, TNF-α, and IL-6 expression profiles were then measured by western blotting. F, G Levels of IL-6 and TNF-α in podocyte cell supernatant. H, I Representative micrographs of CX3CL1 and TNF-α staining in podocytes. Scale bar = 10 μm. (IL-6, interleukin 6; TNF-α, tumor necrosis factor-α; P value was calculated by one-way analysis of variance and Tukey’s test.
9). Chemerin-Induced Down-Regulation of Placenta-Derived Exosomal miR-140-3p and miR-574-3p Promotes Umbilical Vein Endothelial Cells Proliferation, Migration, and Tube Formation in Gestational Diabetes Mellitus. Cells, 2022
(PubMed: 36359855)
[IF=6.0]
10). Mammary Epithelial Cell-Derived Exosomal miR-221-3p Regulates Macrophage Polarization by Targeting Igf2bp2 during Mastitis. Journal of Agricultural and Food Chemistry, 2023
(PubMed: 37757458)
[IF=5.7]
Restrictive clause
Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.
For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.