GPR61 Antibody | Affinity Biosciences

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GPR61 Antibody - Western blot analysis of GPR61 expression in A431 cell lysates .

Product:	GPR61 Antibody
Catalog:	DF2810
Description:	Rabbit polyclonal antibody to GPR61
Application:	WB
Reactivity:	Human, Mouse
Prediction:	Pig, Bovine, Horse
Mol.Wt.:	49 kDa; 49kD(Calculated).
Uniprot:	Q9BZJ8
RRID:	AB_2840016

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse

Prediction:

Pig(100%), Bovine(100%), Horse(100%)

Clonality:

Polyclonal

Specificity:

GPR61 Antibody detects endogenous levels of total GPR61.

RRID:

AB_2840016
Cite Format: Affinity Biosciences Cat# DF2810, RRID:AB_2840016.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

BALGR; Biogenic amine receptor like G protein coupled receptor; Biogenic amine receptor-like G-protein coupled receptor; G protein coupled receptor 61; GPCR3; Gpr61; GPR61_HUMAN; Probable G-protein coupled receptor 61;

Immunogens

Immunogen:

Uniprot:

Q9BZJ8(GPR61_HUMAN) >>Visit HPA database.

Gene(ID):

GPR61(83873)

Expression:

Q9BZJ8 GPR61_HUMAN:

Expressed in brain; detected in frontal and temporal lobes, occipital pole, amygdala and hippocampus (PubMed:11690637, PubMed:29226084). Also expressed in testis (PubMed:11690637, PubMed:29226084) and T cells, B cells, and monocyte (PubMed:29226084). Low expression in many other tissues (PubMed:11690637, PubMed:29226084). Widely expressed in the hippocampus (at protein level).

Description:

Orphan receptor;

Sequence:

Q9BZJ8 GPR61_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MESSPIPQSSGNSSTLGRVPQTPGPSTASGVPEVGLRDVASESVALFFMLLLDLTAVAGNAAVMAVIAKTPALRKFVFVFHLCLVDLLAALTLMPLAMLSSSALFDHALFGEVACRLYLFLSVCFVSLAILSVSAINVERYYYVVHPMRYEVRMTLGLVASVLVGVWVKALAMASVPVLGRVSWEEGAPSVPPGCSLQWSHSAYCQLFVVVFAVLYFLLPLLLILVVYCSMFRVARVAAMQHGPLPTWMETPRQRSESLSSRSTMVTSSGAPQTTPHRTFGGGKAAVVLLAVGGQFLLCWLPYFSFHLYVALSAQPISTGQVESVVTWIGYFCFTSNPFFYGCLNRQIRGELSKQFVCFFKPAPEEELRLPSREGSIEENFLQFLQGTGCPSESWVSRPLPSPKQEPPAVDFRIPGQIAEETSEFLEQQLTSDIIMSDSYLRPAASPRLES

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Bovine

100

Sheep

Dog

Xenopus

Zebrafish

Chicken

Rabbit

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9BZJ8 As Substrate

Q9BZJ8

Site	PTM Type	Enzyme	Source
N12	N-Glycosylation		Uniprot
S161	Phosphorylation		Uniprot

Research Backgrounds

Q9BZJ8_GPR61_HUMAN

Function:

Orphan G-protein coupled receptor. Constitutively activates the G(s)-alpha/cAMP signaling pathway. Shows a reciprocal regulatory interaction with the melatonin receptor MTNR1B most likely through receptor heteromerization. May be involved in the regulation of food intake and body weight (By similarity).

Subcellular Location:

Cell membrane>Multi-pass membrane protein. Endosome membrane>Multi-pass membrane protein.
Note: Colocalizes with ARRB2/beta-arrestin-2 in the endosome (PubMed:28827538).

Tissue Specificity:

Expressed in brain; detected in frontal and temporal lobes, occipital pole, amygdala and hippocampus. Also expressed in testis and T cells, B cells, and monocyte. Low expression in many other tissues. Widely expressed in the hippocampus (at protein level).

Subunit Structure:

Forms heterodimer with MTNR1B. Interacts with ARRB1 and ARRB2 in a spontaneous and agonist-independent manner; leading to the internalization of GPR61 in the endosomal compartment.

Family&Domains:

Belongs to the G-protein coupled receptor 1 family.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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GPR61 Antibody - #DF2810