Product: GPR97 Antibody
Catalog: DF2813
Description: Rabbit polyclonal antibody to GPR97
Application: WB IHC
Reactivity: Human
Prediction: Horse, Rabbit, Dog
Mol.Wt.: 61 kDa; 61kD(Calculated).
Uniprot: Q86Y34
RRID: AB_2840019

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Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Prediction:
Horse(86%), Rabbit(86%), Dog(86%)
Clonality:
Polyclonal
Specificity:
GPR97 Antibody detects endogenous levels of total GPR97.
RRID:
AB_2840019
Cite Format: Affinity Biosciences Cat# DF2813, RRID:AB_2840019.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

EGF-TM7-like; G protein coupled receptor 97; G-protein coupled receptor PGR26; GPR97; GPR97_HUMAN; Pb 99; Pb99; PGR 26; PGR26; Probable G-protein coupled receptor 97;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
Q86Y34 AGRG3_HUMAN:

Expressed in cultured primary dermal lymphatic endothelial cells.

Description:
Orphan receptor;
Sequence:
MATPRGLGALLLLLLLPTSGQEKPTEGPRNTCLGSNNMYDIFNLNDKALCFTKCRQSGSDSCNVENLQRYWLNYEAHLMKEGLTQKVNTPFLKALVQNLSTNTAEDFYFSLEPSQVPRQVMKDEDKPPDRVRLPKSLFRSLPGNRSVVRLAVTILDIGPGTLFKGPRLGLGDGSGVLNNRLVGLSVGQMHVTKLAEPLEIVFSHQRPPPNMTLTCVFWDVTKGTTGDWSSEGCSTEVRPEGTVCCCDHLTFFALLLRPTLDQSTVHILTRISQAGCGVSMIFLAFTIILYAFLRLSRERFKSEDAPKIHVALGGSLFLLNLAFLVNVGSGSKGSDAACWARGAVFHYFLLCAFTWMGLEAFHLYLLAVRVFNTYFGHYFLKLSLVGWGLPALMVIGTGSANSYGLYTIRDRENRTSLELCWFREGTTMYALYITVHGYFLITFLFGMVVLALVVWKIFTLSRATAVKERGKNRKKVLTLLGLSSLVGVTWGLAIFTPLGLSTVYIFALFNSLQGVFICCWFTILYLPSQSTTVSSSTARLDQAHSASQE

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
86
Dog
86
Rabbit
86
Sheep
75
Bovine
71
Pig
57
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q86Y34 As Substrate

Site PTM Type Enzyme
Y70 Phosphorylation
Y347 Phosphorylation
K471 Acetylation
K474 Acetylation
K475 Acetylation
S547 Phosphorylation

Research Backgrounds

Function:

Orphan receptor that regulates migration of lymphatic endothelial cells in vitro via the small GTPases RhoA and CDC42. Regulates B-cell development (By similarity). Seems to signal through G-alpha(q)-proteins.

Subcellular Location:

Cell membrane>Multi-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in cultured primary dermal lymphatic endothelial cells.

Family&Domains:

Belongs to the G-protein coupled receptor 2 family. Adhesion G-protein coupled receptor (ADGR) subfamily.

Restrictive clause

 

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