LPHN3 Antibody | Affinity Biosciences

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LPHN3 Antibody - Western blot analysis of LPHN3 expression in HEK293 cells .

Product:	LPHN3 Antibody
Catalog:	DF2817
Description:	Rabbit polyclonal antibody to LPHN3
Application:	WB
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.:	120 kDa,; 162kD(Calculated).
Uniprot:	Q9HAR2
RRID:	AB_2840023

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%)

Clonality:

Polyclonal

Specificity:

LPHN3 Antibody detects endogenous levels of total LPHN3.

RRID:

AB_2840023
Cite Format: Affinity Biosciences Cat# DF2817, RRID:AB_2840023.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

ADGRL3; Adhesion G protein coupled receptor L3; Calcium independent alpha latrotoxin receptor 3; Calcium-independent alpha-latrotoxin receptor 3; CIRL 3; CIRL-3; CIRL3; CL3; cl3 latrotoxin receptor; KIAA0768; Latrophilin 3; Latrophilin homolog 3 (cow); Latrophilin homolog 3; Latrophilin-3; Latrophilin3; LEC 3; LEC3; Lectomedin 3; Lectomedin-3; Lectomedin3; lph3; LPHN 3; Lphn3; LPHN3_HUMAN;

Immunogens

Immunogen:

Uniprot:

Q9HAR2(AGRL3_HUMAN) >>Visit HPA database.

Gene(ID):

ADGRL3(23284)

Sequence:

Q9HAR2 AGRL3_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MWPSQLLIFMMLLAPIIHAFSRAPIPMAVVRRELSCESYPIELRCPGTDVIMIESANYGRTDDKICDSDPAQMENIRCYLPDAYKIMSQRCNNRTQCAVVAGPDVFPDPCPGTYKYLEVQYECVPYKVEQKVFLCPGLLKGVYQSEHLFESDHQSGAWCKDPLQASDKIYYMPWTPYRTDTLTEYSSKDDFIAGRPTTTYKLPHRVDGTGFVVYDGALFFNKERTRNIVKFDLRTRIKSGEAIIANANYHDTSPYRWGGKSDIDLAVDENGLWVIYATEQNNGKIVISQLNPYTLRIEGTWDTAYDKRSASNAFMICGILYVVKSVYEDDDNEATGNKIDYIYNTDQSKDSLVDVPFPNSYQYIAAVDYNPRDNLLYVWNNYHVVKYSLDFGPLDSRSGQAHHGQVSYISPPIHLDSELERPSVKDISTTGPLGMGSTTTSTTLRTTTLSPGRSTTPSVSGRRNRSTSTPSPAVEVLDDMTTHLPSASSQIPALEESCEAVEAREIMWFKTRQGQIAKQPCPAGTIGVSTYLCLAPDGIWDPQGPDLSNCSSPWVNHITQKLKSGETAANIARELAEQTRNHLNAGDITYSVRAMDQLVGLLDVQLRNLTPGGKDSAARSLNKAMVETVNNLLQPQALNAWRDLTTSDQLRAATMLLHTVEESAFVLADNLLKTDIVRENTDNIKLEVARLSTEGNLEDLKFPENMGHGSTIQLSANTLKQNGRNGEIRVAFVLYNNLGPYLSTENASMKLGTEALSTNHSVIVNSPVITAAINKEFSNKVYLADPVVFTVKHIKQSEENFNPNCSFWSYSKRTMTGYWSTQGCRLLTTNKTHTTCSCNHLTNFAVLMAHVEVKHSDAVHDLLLDVITWVGILLSLVCLLICIFTFCFFRGLQSDRNTIHKNLCISLFVAELLFLIGINRTDQPIACAVFAALLHFFFLAAFTWMFLEGVQLYIMLVEVFESEHSRRKYFYLVGYGMPALIVAVSAAVDYRSYGTDKVCWLRLDTYFIWSFIGPATLIIMLNVIFLGIALYKMFHHTAILKPESGCLDNIKSWVIGAIALLCLLGLTWAFGLMYINESTVIMAYLFTIFNSLQGMFIFIFHCVLQKKVRKEYGKCLRTHCCSGKSTESSIGSGKTSGSRTPGRYSTGSQSRIRRMWNDTVRKQSESSFITGDINSSASLNREGLLNNARDTSVMDTLPLNGNHGNSYSIASGEYLSNCVQIIDRGYNHNETALEKKILKELTSNYIPSYLNNHERSSEQNRNLMNKLVNNLGSGREDDAIVLDDATSFNHEESLGLELIHEESDAPLLPPRVYSTENHQPHHYTRRRIPQDHSESFFPLLTNEHTEDLQSPHRDSLYTSMPTLAGVAATESVTTSTQTEPPPAKCGDAEDVYYKSMPNLGSRNHVHQLHTYYQLGRGSSDGFIVPPNKDGTPPEGSSKGPAHLVTSL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Bovine

100

Sheep

100

Dog

100

Chicken

100

Rabbit

100

Xenopus

Zebrafish

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9HAR2 As Substrate

Q9HAR2

Site	PTM Type	Source
S4	Phosphorylation	Uniprot
S21	Phosphorylation	Uniprot
S35	Phosphorylation	Uniprot
S410	O-Glycosylation	Uniprot
S423	O-Glycosylation	Uniprot
S450	Phosphorylation	Uniprot
T455	Phosphorylation	Uniprot
T456	Phosphorylation	Uniprot
S460	Phosphorylation	Uniprot
T610	Phosphorylation	Uniprot
S663	Phosphorylation	Uniprot
T814	Phosphorylation	Uniprot
T816	Phosphorylation	Uniprot
T821	Phosphorylation	Uniprot
Y990	Phosphorylation	Uniprot
T1140	Phosphorylation	Uniprot
S1148	Phosphorylation	Uniprot
T1159	Phosphorylation	Uniprot
S1175	Phosphorylation	Uniprot
S1176	Phosphorylation	Uniprot
S1178	Phosphorylation	Uniprot
Y1226	Phosphorylation	Uniprot
K1239	Ubiquitination	Uniprot
Y1392	Phosphorylation	Uniprot
S1395	Phosphorylation	Uniprot
Y1411	Phosphorylation	Uniprot
Y1412	Phosphorylation	Uniprot
T1431	Phosphorylation	Uniprot

Research Backgrounds

Q9HAR2_AGRL3_HUMAN

Function:

Plays a role in cell-cell adhesion and neuron guidance via its interactions with FLRT2 and FLRT3 that are expressed at the surface of adjacent cells. Plays a role in the development of glutamatergic synapses in the cortex. Important in determining the connectivity rates between the principal neurons in the cortex.

PTMs:

Proteolytically cleaved into 2 subunits, an extracellular subunit and a seven-transmembrane subunit.

O-glycosylated (major) and N-glycosylated.

Subcellular Location:

Cell membrane>Multi-pass membrane protein. Cell projection>Axon. Cell junction.

Subunit Structure:

Interacts (via olfactomedin-like domain) with FLRT3 (via extracellular domain); the interaction is direct. Identified in a complex with FLRT3 and UNC5B; does not interact with UNC5B by itself. Identified in a complex with FLRT3 and UNC5D; does not interact with UNC5D by itself. Interacts (via olfactomedin-like domain) with FLRT1 (via extracellular domain). Interacts (via olfactomedin-like domain) with FLRT2 (via extracellular domain). Interacts (via extracellular domain) with TENM1. Interacts (via extracellular domain) with TENM3 (By similarity).

Family&Domains:

The Olfactomedin-like domain is required for the synapse-promoting function and the interaction with FLRT3. The Olfactomedin-like and the SUEL-type lectin domains are required for the interaction with TENM1 (By similarity).

Belongs to the G-protein coupled receptor 2 family. LN-TM7 subfamily.

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LPHN3 Antibody - #DF2817