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Product Info

Source:
Mouse
Application:
ELISA 1:10000, WB 1:500-1:2000, IHC 1:200-1:1000, IF/ICC 1:200-1:1000, FCM 1:200-1:400
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Monkey
Clonality:
Monoclonal [AFB1979]
Specificity:
CK5 antibody detects endogenous levels of total CK5.
RRID:
AB_2834001
Cite Format: Affinity Biosciences Cat# BF0493, RRID:AB_2834001.
Conjugate:
Unconjugated.
Purification:
Affinity-chromatography.
Storage:
Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

58 kDa cytokeratin; CK-5; CK5; Cytokeratin-5; Cytokeratin5; DDD; DDD1; EBS2; epidermolysis bullosa simplex 2 Dowling-Meara/Kobner/Weber-Cockayne types; K2C5_HUMAN; K5; keratin 5 (epidermolysis bullosa simplex, Dowling-Meara/Kobner/Weber-Cockayne types); Keratin 5; Keratin; keratin complex 2, basic, gene 5; keratin, type II cytoskeletal 5; Keratin-5; Keratin5; KRT 5; Krt5; KRT5A; type II cytoskeletal 5; Type-II keratin Kb5;

Immunogens

Immunogen:

Purified recombinant fragment of human CK5 expressed in E. Coli.

Uniprot:
Gene(ID):
Description:
The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the basal layer of the epidermis with family member KRT14. Mutations in these genes have been associated with a complex of diseases termed epidermolysis bullosa simplex. The type II cytokeratins are clustered in a region of chromosome 12q12-q13.
Sequence:
MSRQSSVSFRSGGSRSFSTASAITPSVSRTSFTSVSRSGGGGGGGFGRVSLAGACGVGGYGSRSLYNLGGSKRISISTSGGSFRNRFGAGAGGGYGFGGGAGSGFGFGGGAGGGFGLGGGAGFGGGFGGPGFPVCPPGGIQEVTVNQSLLTPLNLQIDPSIQRVRTEEREQIKTLNNKFASFIDKVRFLEQQNKVLDTKWTLLQEQGTKTVRQNLEPLFEQYINNLRRQLDSIVGERGRLDSELRNMQDLVEDFKNKYEDEINKRTTAENEFVMLKKDVDAAYMNKVELEAKVDALMDEINFMKMFFDAELSQMQTHVSDTSVVLSMDNNRNLDLDSIIAEVKAQYEEIANRSRTEAESWYQTKYEELQQTAGRHGDDLRNTKHEISEMNRMIQRLRAEIDNVKKQCANLQNAIADAEQRGELALKDARNKLAELEEALQKAKQDMARLLREYQELMNTKLALDVEIATYRKLLEGEECRLSGEGVGPVNISVVTSSVSSGYGSGSGYGGGLGGGLGGGLGGGLAGGSSGSYYSSSSGGVGLGGGLSVGGSGFSASSGRGLGVGFGSGGGSSSSVKFVSTTSSSRKSFKS

PTMs - P13647 As Substrate

Site PTM Type Enzyme
S2 Phosphorylation
S5 Phosphorylation
S8 Phosphorylation
S11 Phosphorylation
S14 Phosphorylation
S16 Phosphorylation
S18 Phosphorylation
T19 Phosphorylation
S21 Phosphorylation
T24 Phosphorylation
S26 Phosphorylation
S28 Phosphorylation
S31 Phosphorylation
S36 Phosphorylation
S38 Phosphorylation
S50 Phosphorylation
Y60 Phosphorylation
S62 Phosphorylation
S64 Phosphorylation
Y66 Phosphorylation
S71 Phosphorylation
K72 Acetylation
K72 Methylation
S75 Phosphorylation
S77 Phosphorylation
T78 Phosphorylation
S82 Phosphorylation
T151 Phosphorylation
K173 Ubiquitination
T174 Phosphorylation
K178 Acetylation
K178 Methylation
K178 Sumoylation
K178 Ubiquitination
S181 Phosphorylation
K185 Acetylation
K185 Ubiquitination
K199 Methylation
Y222 Phosphorylation
S242 Phosphorylation
K257 Methylation
K257 Ubiquitination
Y258 Phosphorylation
K264 Methylation
K264 Ubiquitination
Y283 Phosphorylation
S337 Phosphorylation
Y346 Phosphorylation
S353 Phosphorylation
T355 Phosphorylation
S359 Phosphorylation
Y361 Phosphorylation
T363 Phosphorylation
Y365 Phosphorylation
K426 Ubiquitination
K443 Sumoylation
Y453 Phosphorylation
R480 Methylation
S567 Phosphorylation
S571 Phosphorylation
S574 Phosphorylation
S579 Phosphorylation
S587 Phosphorylation
S590 Phosphorylation

Research Backgrounds

Subunit Structure:

Heterodimer of a type I and a type II keratin. Heterodimer with type I keratin KRT25 leading to the formation of keratin intermediate filament (KIF) network. Heterodimer with KRT14. Interacts with TCHP. Interacts with EPPK1 (By similarity).

Family&Domains:

Belongs to the intermediate filament family.

References

1). Thymosin β4 Regulates the Differentiation of Thymocytes by Controlling the Cytoskeletal Rearrangement and Mitochondrial Transfer of Thymus Epithelial Cells. International journal of molecular sciences (PubMed: 38256161) [IF=5.6]

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