Product: CD6 Antibody
Catalog: BF0044
Description: Mouse monoclonal antibody to CD6
Application: WB IHC ELISA
Reactivity: Human
Mol.Wt.: 71.8kDa; 72kD(Calculated).
Uniprot: P30203
RRID: AB_2834050

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Product Info

ELISA 1:10000, WB 1:500-1:2000, IHC 1:200-1:1000
*The optimal dilutions should be determined by the end user.

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Monoclonal [AFB2028]
CD6 antibody detects endogenous levels of total CD6.
Cite Format: Affinity Biosciences Cat# BF0044, RRID:AB_2834050.
Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.


CD_antigen=CD6; CD6; CD6 antigen Tp120; CD6 molecule; CD6_HUMAN; FLJ44171; OX52; T cell differentiation antigen CD6; T-cell differentiation antigen CD6; T12; TP120;



Purified recombinant fragment of human CD6 expressed in E. Coli.

P30203 CD6_HUMAN:

Detected on thymocytes (PubMed:15294938). Detected on peripheral blood T-cells (PubMed:15048703, PubMed:16352806). Detected on natural killer (NK) cells (PubMed:16352806). Soluble CD6 is detected in blood serum (at protein level) (PubMed:17601777). Detected in spleen, thymus, appendix, lymph node and peripheral blood leukocytes (PubMed:9013954). Expressed by thymocytes, mature T-cells, a subset of B-cells known as B-1 cells, and by some cells in the brain.

This gene encodes a protein found on the outer membrane of T-lymphocytes as well as some other immune cells. The encoded protein contains three scavenger receptor cysteine-rich (SRCR) domains and a binding site for an activated leukocyte cell adhesion molecule.

PTMs - P30203 As Substrate

Site PTM Type Enzyme
N28 N-Glycosylation
T48 Phosphorylation
N49 N-Glycosylation
N229 N-Glycosylation
Y327 Phosphorylation
S341 Phosphorylation
S396 Phosphorylation
S405 Phosphorylation
S480 Phosphorylation P68400 (CSNK2A1)
S482 Phosphorylation P68400 (CSNK2A1)
S484 Phosphorylation P68400 (CSNK2A1)
S505 Phosphorylation
T511 Phosphorylation
S560 Phosphorylation
S562 Phosphorylation
S565 Phosphorylation
S567 Phosphorylation
S568 Phosphorylation
Y629 Phosphorylation
Y662 Phosphorylation

Research Backgrounds


Cell adhesion molecule that mediates cell-cell contacts and regulates T-cell responses via its interaction with ALCAM/CD166. Contributes to signaling cascades triggered by activation of the TCR/CD3 complex. Functions as costimulatory molecule; promotes T-cell activation and proliferation. Contributes to the formation and maturation of the immunological synapse. Functions as calcium-dependent pattern receptor that binds and aggregates both Gram-positive and Gram-negative bacteria. Binds both lipopolysaccharide (LPS) from Gram-negative bacteria and lipoteichoic acid from Gram-positive bacteria. LPS binding leads to the activation of signaling cascades and down-stream MAP kinases. Mediates activation of the inflammatory response and the secretion of pro-inflammatory cytokines in response to LPS.


After T-cell activation, becomes hyperphosphorylated on Ser and Thr residues and phosphorylated on Tyr residues.


Subcellular Location:

Cell membrane>Single-pass type I membrane protein.
Note: Detected at the immunological synapse, i.e, at the contact zone between antigen-presenting dendritic cells and T-cells (PubMed:15294938, PubMed:16352806). Colocalizes with the TCR/CD3 complex at the immunological synapse (PubMed:15294938).

Note: The origins of the secreted form are not clear, but it might be created by proteolytic shedding of the ectodomain.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Detected on thymocytes. Detected on peripheral blood T-cells. Detected on natural killer (NK) cells. Soluble CD6 is detected in blood serum (at protein level). Detected in spleen, thymus, appendix, lymph node and peripheral blood leukocytes. Expressed by thymocytes, mature T-cells, a subset of B-cells known as B-1 cells, and by some cells in the brain.

Subunit Structure:

Interacts (via extracellular domain) with ALCAM/CD166 (via extracellular domain). Interacts with the TCR/CD3 complex subunit CD3E. Interacts (via tyrosine phosphorylated C-terminus) with LCP2 (via SH2 domain). Interacts with VAV1 (By similarity). Interacts (via glycosylated extracellular domain) with LGALS1 and LGALS3. Interaction with LGALS1 or LGALS3 inhibits interaction with ALCAM.

Research Fields

· Environmental Information Processing > Signaling molecules and interaction > Cell adhesion molecules (CAMs).   (View pathway)

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