T Antibody - #BF0015

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Product: T Antibody
Catalog: BF0015
Description: Mouse monoclonal antibody to T
Application: WB IHC ELISA FACS
Reactivity: Human
Mol.Wt.: 47.4kDa; 47kD(Calculated).
Uniprot: O15178
RRID: AB_2834067

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Related Downloads
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Mouse
Application:
ELISA 1:10000, WB 1:500-1:2000, IHC 1:200-1:1000, FCM 1:200-1:400
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Clonality:
Monoclonal [AFB2045]
Specificity:
T antibody detects endogenous levels of total T.
RRID:
AB_2834067
Cite Format: Affinity Biosciences Cat# BF0015, RRID:AB_2834067.
Conjugate:
Unconjugated.
Purification:
Affinity-chromatography.
Storage:
Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

BRAC_HUMAN; Brachyury homolog; Brachyury protein; Bry; MGC104817; Protein T; SAVA; T; T brachyury homolog; T brachyury transcription factor; T Protein; T, brachyury homolog (mouse); TFT; Transcription factor T;

Immunogens

Immunogen:

Purified recombinant fragment of human T expressed in E. Coli.

Uniprot:

O15178(TBXT_HUMAN) >>Visit HPA database.

Gene(ID):
T(6862)
Expression:
O15178 TBXT_HUMAN:

Detected in testis, but not in other, normal tissues. Detected in lung tumors (at protein level).

Description:
The protein encoded by this gene is an embryonic nuclear transcription factor that binds to a specific DNA element, the palindromic T-site. It binds through a region in its N-terminus, called the T-box, and effects transcription of genes required for mesoderm formation and differentiation. The protein is localized to notochord-derived cells.
Sequence:
MSSPGTESAGKSLQYRVDHLLSAVENELQAGSEKGDPTERELRVGLEESELWLRFKELTNEMIVTKNGRRMFPVLKVNVSGLDPNAMYSFLLDFVAADNHRWKYVNGEWVPGGKPEPQAPSCVYIHPDSPNFGAHWMKAPVSFSKVKLTNKLNGGGQIMLNSLHKYEPRIHIVRVGGPQRMITSHCFPETQFIAVTAYQNEEITALKIKYNPFAKAFLDAKERSDHKEMMEEPGDSQQPGYSQWGWLLPGTSTLCPPANPHPQFGGALSLPSTHSCDRYPTLRSHRSSPYPSPYAHRNNSPTYSDNSPACLSMLQSHDNWSSLGMPAHPSMLPVSHNASPPTSSSQYPSLWSVSNGAVTPGSQAAAVSNGLGAQFFRGSPAHYTPLTHPVSAPSSSGSPLYEGAAAATDIVDSQYDAAAQGRLIASWTPVSPPSM

PTMs - O15178 As Substrate

Site PTM Type Enzyme
S12 Phosphorylation
Y15 Phosphorylation
T65 Phosphorylation
T149 Phosphorylation
S162 Phosphorylation
S431 Phosphorylation

Research Backgrounds

Function:

Involved in the transcriptional regulation of genes required for mesoderm formation and differentiation. Binds to a palindromic site (called T site) and activates gene transcription when bound to such a site.

Subcellular Location:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Detected in testis, but not in other, normal tissues. Detected in lung tumors (at protein level).

Subunit Structure:

Monomer.

Restrictive clause

 

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For Research Use Only.
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