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Product Info

ELISA 1:10000, WB 1:500-1:2000, IHC 1:200-1:1000, IF/ICC 1:200-1:1000, FCM 1:200-1:400
*The optimal dilutions should be determined by the end user.

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Monoclonal [AFB2055]
PSAP antibody detects endogenous levels of total PSAP.
Cite Format: Affinity Biosciences Cat# BF0001, RRID:AB_2834077.
Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.


A1 activator; Cerebroside sulfate activator; Co-beta-glucosidase; Component C; CSAct; Dispersin; GLBA; Glucosylceramidase activator; Proactivator polypeptide; Proactivator polypeptide precursor; Prosaposin (sphingolipid activator protein 1); prosaposin (variant Gaucher disease and variant metachromatic leukodystrophy); Prosaposin; Protein A; Protein C; PSAP; SAP-1; SAP-2; SAP_HUMAN; SAP1; Saposin A; Saposin B; Saposin B Val; Saposin C; Saposin D; Saposin-D; Saposins; Sgp1; Sphingolipid activator protein 1; Sphingolipid activator protein 2; Sulfated glycoprotein 1; Sulfatide/GM1 activator;



Purified recombinant fragment of human PSAP expressed in E. Coli.

This gene encodes a highly conserved glycoprotein which is a precursor for 4 cleavage products: saposins A, B, C, and D. Each domain of the precursor protein is approximately 80 amino acid residues long with nearly identical placement of cysteine residues and glycosylation sites. Saposins A-D localize primarily to the lysosomal compartment where they facilitate the catabolism of glycosphingolipids with short oligosaccharide groups. The precursor protein exists both as a secretory protein and as an integral membrane protein and has neurotrophic activities. Mutations in this gene have been associated with Gaucher disease, Tay-Sachs disease, and metachromatic leukodystrophy. Alternative splicing results in multiple transcript variants encoding different isoforms.

PTMs - P07602 As Substrate

Site PTM Type Enzyme
S9 Phosphorylation
R27 Methylation
K55 Ubiquitination
T71 Phosphorylation
N80 N-Glycosylation
T82 Phosphorylation
Y89 Phosphorylation
N101 N-Glycosylation
S112 Phosphorylation
Y113 Phosphorylation
K143 Ubiquitination
K152 Ubiquitination
T165 Phosphorylation
Y180 Phosphorylation
S187 Phosphorylation
K290 Ubiquitination
K303 Ubiquitination
K323 Ubiquitination
N332 N-Glycosylation
K336 Ubiquitination
K344 Ubiquitination
T397 O-Glycosylation
T401 O-Glycosylation
K413 Acetylation
K414 Ubiquitination
Y418 Phosphorylation
R421 Methylation
N426 N-Glycosylation
K429 Ubiquitination
K449 Ubiquitination
S473 Phosphorylation
K487 Ubiquitination

Research Backgrounds


Saposin-A and saposin-C stimulate the hydrolysis of glucosylceramide by beta-glucosylceramidase (EC and galactosylceramide by beta-galactosylceramidase (EC Saposin-C apparently acts by combining with the enzyme and acidic lipid to form an activated complex, rather than by solubilizing the substrate.

Saposin-B stimulates the hydrolysis of galacto-cerebroside sulfate by arylsulfatase A (EC, GM1 gangliosides by beta-galactosidase (EC and globotriaosylceramide by alpha-galactosidase A (EC Saposin-B forms a solubilizing complex with the substrates of the sphingolipid hydrolases.

Saposin-D is a specific sphingomyelin phosphodiesterase activator (EC

Behaves as a myelinotrophic and neurotrophic factor, these effects are mediated by its G-protein-coupled receptors, GPR37 and GPR37L1, undergoing ligand-mediated internalization followed by ERK phosphorylation signaling.

Saposins are specific low-molecular mass non-enzymic proteins, they participate in the lysosomal degradation of sphingolipids, which takes place by the sequential action of specific hydrolases.


The lysosomal precursor is proteolytically processed to 4 small peptides, which are similar to each other and are sphingolipid hydrolase activator proteins.

N-linked glycans show a high degree of microheterogeneity.

The one residue extended Saposin-B-Val is only found in 5% of the chains.

Subcellular Location:


Note: Secreted as a fully glycosylated 70 kDa protein composed of complex glycans.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Saposin-B is a homodimer. Prosaposin exists as a roughly half-half mixture of monomers and disulfide-linked dimers. Monomeric prosaposin interacts (via C-terminus) with sortilin/SORT1, the interaction is required for targeting to lysosomes. Interacts with GRN; facilitates lysosomal delivery of progranulin from the extracellular space and the biosynthetic pathway.

Research Fields

· Cellular Processes > Transport and catabolism > Lysosome.   (View pathway)

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