Ki67 Antibody | Affinity Biosciences

Product:	Ki67 Antibody
Catalog:	AF0198
Description:	Rabbit polyclonal antibody to Ki67
Application:	WB IHC IF/ICC
Cited expt.:	WB, IHC, IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Bovine, Rabbit, Dog
Mol.Wt.:	358kDa; 359kD(Calculated).
Uniprot:	P46013
RRID:	AB_2834152

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Bovine(100%), Rabbit(100%), Dog(82%)

Clonality:

Polyclonal

Specificity:

Ki67 Antibody detects endogenous levels of total Ki67.

RRID:

AB_2834152
Cite Format: Affinity Biosciences Cat# AF0198, RRID:AB_2834152.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Antigen identified by monoclonal antibody Ki 67; Antigen identified by monoclonal antibody Ki-67; Antigen KI-67; Antigen KI67; Antigen Ki67; KI67_HUMAN; KIA; Marker of proliferation Ki-67; MIB 1; MIB; MKI67; PPP1R105; Proliferation marker protein Ki-67; Proliferation related Ki 67 antigen; Protein phosphatase 1 regulatory subunit 105; RP11-380J17.2;

Immunogens

Immunogen:

A synthesized peptide derived from human Ki67, corresponding to a region within the internal amino acids.

Uniprot:

P46013(KI67_HUMAN) >>Visit HPA database.

Gene(ID):

MKI67(4288)

Description:

KI-67 a protein that may be a marker of proliferating cells, involved in chromatin compaction. Its expression is altered in many tumor types including osteosarcomas, histiocytomas, prostate, breast and esophageal cancers. Mutated in colon, cervical and lung cancers.

Sequence:

P46013 KI67_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MWPTRRLVTIKRSGVDGPHFPLSLSTCLFGRGIECDIRIQLPVVSKQHCKIEIHEQEAILHNFSSTNPTQVNGSVIDEPVRLKHGDVITIIDRSFRYENESLQNGRKSTEFPRKIREQEPARRVSRSSFSSDPDEKAQDSKAYSKITEGKVSGNPQVHIKNVKEDSTADDSKDSVAQGTTNVHSSEHAGRNGRNAADPISGDFKEISSVKLVSRYGELKSVPTTQCLDNSKKNESPFWKLYESVKKELDVKSQKENVLQYCRKSGLQTDYATEKESADGLQGETQLLVSRKSRPKSGGSGHAVAEPASPEQELDQNKGKGRDVESVQTPSKAVGASFPLYEPAKMKTPVQYSQQQNSPQKHKNKDLYTTGRRESVNLGKSEGFKAGDKTLTPRKLSTRNRTPAKVEDAADSATKPENLSSKTRGSIPTDVEVLPTETEIHNEPFLTLWLTQVERKIQKDSLSKPEKLGTTAGQMCSGLPGLSSVDINNFGDSINESEGIPLKRRRVSFGGHLRPELFDENLPPNTPLKRGEAPTKRKSLVMHTPPVLKKIIKEQPQPSGKQESGSEIHVEVKAQSLVISPPAPSPRKTPVASDQRRRSCKTAPASSSKSQTEVPKRGGRKSGNLPSKRVSISRSQHDILQMICSKRRSGASEANLIVAKSWADVVKLGAKQTQTKVIKHGPQRSMNKRQRRPATPKKPVGEVHSQFSTGHANSPCTIIIGKAHTEKVHVPARPYRVLNNFISNQKMDFKEDLSGIAEMFKTPVKEQPQLTSTCHIAISNSENLLGKQFQGTDSGEEPLLPTSESFGGNVFFSAQNAAKQPSDKCSASPPLRRQCIRENGNVAKTPRNTYKMTSLETKTSDTETEPSKTVSTANRSGRSTEFRNIQKLPVESKSEETNTEIVECILKRGQKATLLQQRREGEMKEIERPFETYKENIELKENDEKMKAMKRSRTWGQKCAPMSDLTDLKSLPDTELMKDTARGQNLLQTQDHAKAPKSEKGKITKMPCQSLQPEPINTPTHTKQQLKASLGKVGVKEELLAVGKFTRTSGETTHTHREPAGDGKSIRTFKESPKQILDPAARVTGMKKWPRTPKEEAQSLEDLAGFKELFQTPGPSEESMTDEKTTKIACKSPPPESVDTPTSTKQWPKRSLRKADVEEEFLALRKLTPSAGKAMLTPKPAGGDEKDIKAFMGTPVQKLDLAGTLPGSKRQLQTPKEKAQALEDLAGFKELFQTPGHTEELVAAGKTTKIPCDSPQSDPVDTPTSTKQRPKRSIRKADVEGELLACRNLMPSAGKAMHTPKPSVGEEKDIIIFVGTPVQKLDLTENLTGSKRRPQTPKEEAQALEDLTGFKELFQTPGHTEEAVAAGKTTKMPCESSPPESADTPTSTRRQPKTPLEKRDVQKELSALKKLTQTSGETTHTDKVPGGEDKSINAFRETAKQKLDPAASVTGSKRHPKTKEKAQPLEDLAGLKELFQTPVCTDKPTTHEKTTKIACRSQPDPVDTPTSSKPQSKRSLRKVDVEEEFFALRKRTPSAGKAMHTPKPAVSGEKNIYAFMGTPVQKLDLTENLTGSKRRLQTPKEKAQALEDLAGFKELFQTRGHTEESMTNDKTAKVACKSSQPDPDKNPASSKRRLKTSLGKVGVKEELLAVGKLTQTSGETTHTHTEPTGDGKSMKAFMESPKQILDSAASLTGSKRQLRTPKGKSEVPEDLAGFIELFQTPSHTKESMTNEKTTKVSYRASQPDLVDTPTSSKPQPKRSLRKADTEEEFLAFRKQTPSAGKAMHTPKPAVGEEKDINTFLGTPVQKLDQPGNLPGSNRRLQTRKEKAQALEELTGFRELFQTPCTDNPTTDEKTTKKILCKSPQSDPADTPTNTKQRPKRSLKKADVEEEFLAFRKLTPSAGKAMHTPKAAVGEEKDINTFVGTPVEKLDLLGNLPGSKRRPQTPKEKAKALEDLAGFKELFQTPGHTEESMTDDKITEVSCKSPQPDPVKTPTSSKQRLKISLGKVGVKEEVLPVGKLTQTSGKTTQTHRETAGDGKSIKAFKESAKQMLDPANYGTGMERWPRTPKEEAQSLEDLAGFKELFQTPDHTEESTTDDKTTKIACKSPPPESMDTPTSTRRRPKTPLGKRDIVEELSALKQLTQTTHTDKVPGDEDKGINVFRETAKQKLDPAASVTGSKRQPRTPKGKAQPLEDLAGLKELFQTPICTDKPTTHEKTTKIACRSPQPDPVGTPTIFKPQSKRSLRKADVEEESLALRKRTPSVGKAMDTPKPAGGDEKDMKAFMGTPVQKLDLPGNLPGSKRWPQTPKEKAQALEDLAGFKELFQTPGTDKPTTDEKTTKIACKSPQPDPVDTPASTKQRPKRNLRKADVEEEFLALRKRTPSAGKAMDTPKPAVSDEKNINTFVETPVQKLDLLGNLPGSKRQPQTPKEKAEALEDLVGFKELFQTPGHTEESMTDDKITEVSCKSPQPESFKTSRSSKQRLKIPLVKVDMKEEPLAVSKLTRTSGETTQTHTEPTGDSKSIKAFKESPKQILDPAASVTGSRRQLRTRKEKARALEDLVDFKELFSAPGHTEESMTIDKNTKIPCKSPPPELTDTATSTKRCPKTRPRKEVKEELSAVERLTQTSGQSTHTHKEPASGDEGIKVLKQRAKKKPNPVEEEPSRRRPRAPKEKAQPLEDLAGFTELSETSGHTQESLTAGKATKIPCESPPLEVVDTTASTKRHLRTRVQKVQVKEEPSAVKFTQTSGETTDADKEPAGEDKGIKALKESAKQTPAPAASVTGSRRRPRAPRESAQAIEDLAGFKDPAAGHTEESMTDDKTTKIPCKSSPELEDTATSSKRRPRTRAQKVEVKEELLAVGKLTQTSGETTHTDKEPVGEGKGTKAFKQPAKRKLDAEDVIGSRRQPRAPKEKAQPLEDLASFQELSQTPGHTEELANGAADSFTSAPKQTPDSGKPLKISRRVLRAPKVEPVGDVVSTRDPVKSQSKSNTSLPPLPFKRGGGKDGSVTGTKRLRCMPAPEEIVEELPASKKQRVAPRARGKSSEPVVIMKRSLRTSAKRIEPAEELNSNDMKTNKEEHKLQDSVPENKGISLRSRRQNKTEAEQQITEVFVLAERIEINRNEKKPMKTSPEMDIQNPDDGARKPIPRDKVTENKRCLRSARQNESSQPKVAEESGGQKSAKVLMQNQKGKGEAGNSDSMCLRSRKTKSQPAASTLESKSVQRVTRSVKRCAENPKKAEDNVCVKKIRTRSHRDSEDI

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Rabbit

100

Bovine

100

Dog

Chicken

Xenopus

Pig

Horse

Sheep

Zebrafish

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

Research Backgrounds

P46013_KI67_HUMAN

Function:

Required to maintain individual mitotic chromosomes dispersed in the cytoplasm following nuclear envelope disassembly. Associates with the surface of the mitotic chromosome, the perichromosomal layer, and covers a substantial fraction of the chromosome surface. Prevents chromosomes from collapsing into a single chromatin mass by forming a steric and electrostatic charge barrier: the protein has a high net electrical charge and acts as a surfactant, dispersing chromosomes and enabling independent chromosome motility. Binds DNA, with a preference for supercoiled DNA and AT-rich DNA. Does not contribute to the internal structure of mitotic chromosomes (By similarity). May play a role in chromatin organization. It is however unclear whether it plays a direct role in chromatin organization or whether it is an indirect consequence of its function in maintaining mitotic chromosomes dispersed (Probable).

PTMs:

Phosphorylated. Hyperphosphorylated in mitosis. Hyperphosphorylated form does not bind DNA.

Subcellular Location:

Chromosome. Nucleus. Nucleus>Nucleolus.
Note: Associates with the surface of the mitotic chromosome, the perichromosomal layer, and covers a substantial fraction of the mitotic chromosome surface (PubMed:27362226). Associates with satellite DNA in G1 phase (PubMed:9510506). Binds tightly to chromatin in interphase, chromatin-binding decreases in mitosis when it associates with the surface of the condensed chromosomes (PubMed:15896774, PubMed:22002106). Predominantly localized in the G1 phase in the perinucleolar region, in the later phases it is also detected throughout the nuclear interior, being predominantly localized in the nuclear matrix (PubMed:22002106).

References

1). AIMP1 promotes multiple myeloma malignancy through interacting with ANP32A to mediate histone H3 acetylation. Cancer Communications, 2022 (PubMed: 36042007) [IF=16.2]

2). Mitochondrial Disruption Nanosystem Simultaneously Depressed Programmed Death Ligand-1 and Transforming Growth Factor-β to Overcome Photodynamic Immunotherapy Resistance. ACS nano, 2024 (PubMed: 38227812) [IF=15.8]

3). Mitochondrial metabolism blockade nanoadjuvant reversed immune-resistance microenvironment to sensitize albumin-bound paclitaxel-based chemo-immunotherapy. Acta pharmaceutica Sinica. B, 2024 (PubMed: 39309498) [IF=14.7]

Application: IHC Species: Mouse Sample: 4T1 cells

Figure 5. TPP-TAM@Alb nanoparticles reduced the expression of PD-L1 and TGF-β protein to amplify T cell infiltration in 4T1 subcutaneous tumors for enhancing the tumor cell killing activity of PTX@Alb. (A, B) The typical image of the PD-L1 and TGF-β proteins in 4T1 tumors detected by Western blot assay after different treatments. (C–E) Quantification of the amounts of CD3+, CD8+, and CD4+ T cells in 4T1 tumors by the flow cytometry measurements after receiving various treatments (n = 5). (F) The representative immunofluorescence images of Ki67 protein, TUNEL, and H&E staining after PTX@Alb, TPP-TAM@Alb, anti-PD-L1 antibody, or PTX@Alb and TPP-TAM@Alb combination therapy, scale bar = 100 μm. (G, H) Quantification of the relative Ki67 and TUNEL mean fluorescence intensity (MFI) after different treatments (n = 3). All data are presented as mean ± SD. ∗P < 0.05 and ∗∗∗P < 0.001.

4). NAT10 promotes cell proliferation by acetylating CEP170 mRNA to enhance translation efficiency in multiple myeloma. Acta Pharmaceutica Sinica B, 2022 (PubMed: 35967285) [IF=14.7]

5). Chemical conjugation mitigates immunotoxicity of chemotherapy via reducing receptor-mediated drug leakage from lipid nanoparticles. Science advances, 2024 (PubMed: 38838152) [IF=11.7]

Application: IF/ICC Species: Mouse Sample:

Fig. 5. Antitumor immunity and immunotoxicity of sHDLs. (A and B) Concentrations of IFN-γ, IL-12p40, and TNF-α (A) and percentage of mature DC (B) in DLN after different treatments. (C to G) Intratumoral density of CD11b+ cells (C), M1-to-M2 ratio (D), densities of CD3+ cells (E), and CD8+IFN-γ+ cells (F) and percentage of Ki67+ cells among CD8+ cells (G) after different treatments. (H and I) Concentrations of CXCL9 (H) and CXCL10 (I) in tumors after different treatments. (J to M) Density of GMP in a single femur (J), amounts of CD11b+ cells (K), and CD3+ cells (L) in blood, and number of CD3+ cells in DLN (M) after different treatments. (N) Immunofluorescence images of DLN collected from mice receiving different treatments. DAPI (blue), Ki67 (red), CD3 (pink), and CD20 (green). Data were presented as mean ± SD (n = 5), and statistical significance was calculated using a two-sided one-way ANOVA test.

6). NLRP3 inflammasome constrains liver regeneration through impairing MerTK-mediated macrophage efferocytosis. Science Advances, 2025 [IF=11.7]

7). BUB1 drives the occurrence and development of bladder cancer by mediating the STAT3 signaling pathway. JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH, 2021 (PubMed: 34852826) [IF=11.3]

8). METTL13 Mediates the Translation of Snail in Head and Neck Squamous Cell Carcinoma. International Journal of Oral Science, 2020 (PubMed: 34381012) [IF=10.8]

Application: IHC Species: mouse Sample: tumor

Fig. 6| METTL13 regulates HNSCC cell proliferation in vivo.d The tumor weigh was measured after sacrificing mice.e Representative images of H&E and immunohistochemistry (IHC) detection of METTL13, Snail, and Ki67 in xenograft studies. Scale bar, 200 μm

Application: IHC Species: mouse Sample: tumor

Fig. 6| METTL13 regulates HNSCC cell proliferation in vivo.e Representative images of H&E and immunohistochemistry (IHC) detection of METTL13, Snail, and Ki67 in xenograft studies. Scale bar, 200 μm

Application: IHC Species: Mice Sample: SCC15 cells

Fig. 6 METTL13 regulates HNSCC cell proliferation in vivo. a BALB/C nude mice (n = 6) were subcutaneously transplanted with SCC15 cells transfected with LV-shRNA-1, LV-shRNA-2, and LV-shCtrl. b Tumors were collected after 4 weeks. c Tumor volume was calculated every 2 days and determined by length × width2/2. d The tumor weigh was measured after sacrificing mice. e Representative images of H&E and immunohistochemistry (IHC) detection of METTL13, Snail, and Ki67 in xenograft studies. Scale bar, 200 μm

9). Identification of HDAC9 as a viable therapeutic target for the treatment of gastric cancer. EXPERIMENTAL AND MOLECULAR MEDICINE, 2019 (PubMed: 31451695) [IF=9.5]

Application: IHC Species: mouse Sample: SGC-7901cells

Fig. 8| HDAC9 inhibition enhanced the effect of cisplatin on GC tumor suppression.d HDAC9 expression and cell proliferation and apoptosis were assessed by HDAC9 and Ki67 immunohistochemical staining and a TUNEL assay using serial sections. The HDAC9-negative area denoted by the dotted line indicates the location of siHDAC9 injection. Scale bars = 100 μm. The error bars indicate the means ± SDs; *p < 0.05, **p < 0.01, ***p < 0.001 versus the control group

10). Graphene foam/hydrogel scaffolds for regeneration of peripheral nerve using ADSCs in a diabetic mouse model. Nano Research, 2021 [IF=9.5]

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Ki67 Antibody - #AF0198