MAST3 Antibody - #AF0497

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Product: MAST3 Antibody
Catalog: AF0497
Description: Rabbit polyclonal antibody to MAST3
Application: WB IHC IF/ICC
Reactivity: Human, Mouse
Prediction: Pig, Bovine, Sheep, Dog
Mol.Wt.: 143kDa; 143kD(Calculated).
Uniprot: O60307
RRID: AB_2834177

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Pig(100%), Bovine(100%), Sheep(100%), Dog(100%)
Clonality:
Polyclonal
Specificity:
MAST3 Antibody detects endogenous levels of total MAST3.
RRID:
AB_2834177
Cite Format: Affinity Biosciences Cat# AF0497, RRID:AB_2834177.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

EC 2.7.11.1; KIAA0561; MAST 3; MAST3; MAST3_HUMAN; Microtubule associated serine/threonine protein kinase 3; Microtubule-associated serine/threonine-protein kinase 3;

Immunogens

Immunogen:
Uniprot:

O60307(MAST3_HUMAN) >>Visit HPA database.

Gene(ID):
MAST3(23031)
Description:
MAST3 Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. Interacts with PTEN. Note: This description may include information from UniProtKB.
Sequence:
MDESSLLRRRGLQKELSLPRRGRGCRSGNRKSLVVGTPSPTLSRPLSPLSVPTAGSSPLDSPRNFSAASALNFPFARRADGRRWSLASLPSSGYGTNTPSSTLSSSSSSRERLHQLPFQPTPDELHFLSKHFRSSENVLDEEGGRSPRLRPRSRSLSPGRATGTFDNEIVMMNHVYRERFPKATAQMEGRLQEFLTAYAPGARLALADGVLGFIHHQIVELARDCLAKSGENLVTSRYFLEMQEKLERLLQDAHERSDSEEVSFIVQLVRKLLIIISRPARLLECLEFDPEEFYHLLEAAEGHAREGQGIKTDLPQYIIGQLGLAKDPLEEMVPLSHLEEEQPPAPESPESRALVGQSRRKPCESDFETIKLISNGAYGAVYLVRHRDTRQRFAIKKINKQNLILRNQIQQVFVERDILTFAENPFVVSMFCSFETRRHLCMVMEYVEGGDCATLLKNMGPLPVDMARLYFAETVLALEYLHNYGIVHRDLKPDNLLITSLGHIKLTDFGLSKIGLMSMATNLYEGHIEKDAREFIDKQVCGTPEYIAPEVIFRQGYGKPVDWWAMGVVLYEFLVGCVPFFGDTPEELFGQVVSDEIMWPEGDEALPADAQDLITRLLRQSPLDRLGTGGTHEVKQHPFFLALDWAGLLRHKAEFVPQLEAEDDTSYFDTRSERYRHLGSEDDETNDEESSTEIPQFSSCSHRFSKVYSSSEFLAVQPTPTFAERSFSEDREEGWERSEVDYGRRLSADIRLRSWTSSGSSCQSSSSQPERGPSPSLLNTISLDTMPKFAFSSEDEGVGPGPAGPKRPVFILGEPDPPPAATPVMPKPSSLSADTAALSHARLRSNSIGARHSTPRPLDAGRGRRLGGPRDPAPEKSRASSSGGSGGGSGGRVPKSASVSALSLIITADDGSGGPLMSPLSPRSLSSNPSSRDSSPSRDPSPVCGSLRPPIVIHSSGKKYGFSLRAIRVYMGDSDVYTVHHVVWSVEDGSPAQEAGLRAGDLITHINGESVLGLVHMDVVELLLKSGNKISLRTTALENTSIKVGPARKNVAKGRMARRSKRSRRRETQDRRKSLFKKISKQTSVLHTSRSFSSGLHHSLSSSESLPGSPTHSLSPSPTTPCRSPAPDVPADTTASPPSASPSSSSPASPAAAGHTRPSSLHGLAAKLGPPRPKTGRRKSTSSIPPSPLACPPISAPPPRSPSPLPGHPPAPARSPRLRRGQSADKLGTGERLDGEAGRRTRGPEAELVVMRRLHLSERRDSFKKQEAVQEVSFDEPQEEATGLPTSVPQIAVEGEEAVPVALGPTGRD

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Bovine
100
Sheep
100
Dog
100
Xenopus
73
Horse
0
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O60307 As Substrate

Site PTM Type Enzyme
T37 Phosphorylation
S39 Phosphorylation
S47 Phosphorylation
S66 Phosphorylation
S85 Phosphorylation
S88 Phosphorylation
T121 Phosphorylation
S134 Phosphorylation
S135 Phosphorylation
S146 Phosphorylation
S153 Phosphorylation
S155 Phosphorylation
S157 Phosphorylation
T164 Phosphorylation
Y176 Phosphorylation
S259 Phosphorylation
S277 Phosphorylation
S348 Phosphorylation
K361 Ubiquitination
T369 Phosphorylation
K400 Ubiquitination
S512 Phosphorylation
Y524 Phosphorylation
K530 Ubiquitination
K538 Ubiquitination
T665 Phosphorylation
S672 Phosphorylation
S680 Phosphorylation
T685 Phosphorylation
S698 Phosphorylation
S699 Phosphorylation
Y708 Phosphorylation
S709 Phosphorylation
S710 Phosphorylation
S711 Phosphorylation
S726 Phosphorylation
S728 Phosphorylation
S747 Phosphorylation
S754 Phosphorylation
S760 Phosphorylation
S774 Phosphorylation
S782 Phosphorylation
S792 Phosphorylation
S793 Phosphorylation
S847 Phosphorylation
R878 Methylation
S880 Phosphorylation
S885 Phosphorylation
S889 Phosphorylation
R892 Methylation
S935 Phosphorylation
S941 Phosphorylation
S963 Phosphorylation
Y977 Phosphorylation
S1060 Phosphorylation
S1063 Phosphorylation
K1081 Ubiquitination
S1084 Phosphorylation
S1091 Phosphorylation
S1102 Phosphorylation
S1136 Phosphorylation
S1201 Phosphorylation
S1203 Phosphorylation
S1215 Phosphorylation
S1223 Phosphorylation
S1257 Phosphorylation
S1262 Phosphorylation
S1273 Phosphorylation

Research Backgrounds

Subunit Structure:

Interacts with PTEN.

Family&Domains:

Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.

Restrictive clause

 

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