HEXIM1 Antibody - #AF0568

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Product: HEXIM1 Antibody
Catalog: AF0568
Description: Rabbit polyclonal antibody to HEXIM1
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat, Monkey
Prediction: Bovine, Horse, Rabbit, Dog
Mol.Wt.: 40kDa, 70 kDa; 41kD(Calculated).
Uniprot: O94992
RRID: AB_2834193

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat,Monkey
Prediction:
Bovine(91%), Horse(100%), Rabbit(100%), Dog(100%)
Clonality:
Polyclonal
Specificity:
HEXIM1 Antibody detects endogenous levels of total HEXIM1.
RRID:
AB_2834193
Cite Format: Affinity Biosciences Cat# AF0568, RRID:AB_2834193.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Cardiac lineage protein 1; CLP 1; CLP1; EDG 1; EDG1; Estrogen down-regulated gene 1 protein; FLJ13562; Hexamethylene bis acetamide inducible 1; Hexamethylene bis acetamide inducible protein; Hexamethylene bis acetamide inducible transcript 1; Hexamethylene bis-acetamide-inducible protein 1; HEXI1_HUMAN; HEXIM 1; Hexim1; HEXIM1 protein; HIS 1; HIS1; HMBA inducible; MAQ 1; MAQ1; Menage a quatre 1; Menage a quatre protein 1; Protein HEXIM1;

Immunogens

Immunogen:
Uniprot:

O94992(HEXI1_HUMAN) >>Visit HPA database.

Gene(ID):
HEXIM1(10614)
Expression:
O94992 HEXI1_HUMAN:

Ubiquitously expressed with higher expression in placenta. HEXIM1 and HEXIM2 are differentially expressed. Expressed in endocrine tissues.

Description:
HEXIM1 Transcriptional regulator which functions as a general RNA polymerase II transcription inhibitor. In cooperation with 7SK snRNA sequesters P-TEFb in a large inactive 7SK snRNP complex preventing RNA polymerase II phosphorylation and subsequent transcriptional elongation. May also regulate NF-kappa-B, ESR1, NR3C1 and CIITA-dependent transcriptional activity. Homooligomer and heterooligomer with HEXIM2; probably dimeric. Component of the 7SK snRNP complex at least composed of P-TEFb (composed of CDK9 and CCNT1/cyclin-T1), HEXIM1, HEXIM2, BCDIN3, SART3 proteins and 7SK and U6 snRNAs. Interacts with the N-CoR complex through NCOR1.
Sequence:
MAEPFLSEYQHQPQTSNCTGAAAVQEELNPERPPGAEERVPEEDSRWQSRAFPQLGGRPGPEGEGSLESQPPPLQTQACPESSCLREGEKGQNGDDSSAGGDFPPPAEVEPTPEAELLAQPCHDSEASKLGAPAAGGEEEWGQQQRQLGKKKHRRRPSKKKRHWKPYYKLTWEEKKKFDEKQSLRASRIRAEMFAKGQPVAPYNTTQFLMDDHDQEEPDLKTGLYSKRAAAKSDDTSDDDFMEEGGEEDGGSDGMGGDGSEFLQRDFSETYERYHTESLQNMSKQELIKEYLELEKCLSRMEDENNRLRLESKRLGGDDARVRELELELDRLRAENLQLLTENELHRQQERAPLSKFGD

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Dog
100
Rabbit
100
Bovine
91
Pig
0
Sheep
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O94992 As Substrate

Site PTM Type Enzyme
S66 Phosphorylation
S69 Phosphorylation
S97 Phosphorylation
S98 Phosphorylation
R146 Methylation
K150 Ubiquitination
S158 Phosphorylation P17252 (PRKCA)
K165 Acetylation
Y167 Phosphorylation
Y168 Phosphorylation
K169 Ubiquitination
K175 Ubiquitination
K181 Ubiquitination
S183 Phosphorylation
K196 Ubiquitination
T206 Phosphorylation
K221 Ubiquitination
S226 Phosphorylation
K227 Ubiquitination
S233 Phosphorylation
T236 Phosphorylation
S237 Phosphorylation
S252 Phosphorylation
S260 Phosphorylation
S268 Phosphorylation
T270 Phosphorylation
Y271 Phosphorylation
R273 Methylation
Y274 Phosphorylation
T276 Phosphorylation
S278 Phosphorylation
S283 Phosphorylation
K284 Acetylation
K284 Ubiquitination
K289 Acetylation
K289 Ubiquitination
Y291 Phosphorylation
K296 Acetylation
K296 Methylation
K296 Ubiquitination
R331 Methylation
T341 Phosphorylation
R347 Methylation
S355 Phosphorylation
K356 Ubiquitination

Research Backgrounds

Function:

Transcriptional regulator which functions as a general RNA polymerase II transcription inhibitor. In cooperation with 7SK snRNA sequesters P-TEFb in a large inactive 7SK snRNP complex preventing RNA polymerase II phosphorylation and subsequent transcriptional elongation. May also regulate NF-kappa-B, ESR1, NR3C1 and CIITA-dependent transcriptional activity. Plays a role in the regulation of DNA virus-mediated innate immune response by assembling into the HDP-RNP complex, a complex that serves as a platform for IRF3 phosphorylation and subsequent innate immune response activation through the cGAS-STING pathway.

Subcellular Location:

Nucleus. Cytoplasm.
Note: Binds alpha-importin and is mostly nuclear (PubMed:16362050).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Ubiquitously expressed with higher expression in placenta. HEXIM1 and HEXIM2 are differentially expressed. Expressed in endocrine tissues.

Subunit Structure:

Homooligomer and heterooligomer with HEXIM2; probably dimeric. Component of the 7SK snRNP complex at least composed of P-TEFb (composed of CDK9 and CCNT1/cyclin-T1), HEXIM1, HEXIM2, BCDIN3, SART3 proteins and 7SK and U6 snRNAs. Interacts with the N-CoR complex through NCOR1. Interacts with ESR1 and NR3C1. May interact with NF-kappa-B through RELA. Interacts with CCNT2; mediates formation of a tripartite complex with KPNA2. Part of the HDP-RNP complex composed of at least HEXIM1, PRKDC, XRCC5, XRCC6, paraspeckle proteins (SFPQ, NONO, PSPC1, RBM14, and MATR3) and NEAT1 non-coding RNA.

Family&Domains:

The coiled-coil domain mediates oligomerization.

Belongs to the HEXIM family.

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