TRIM16 Antibody - #AF0352

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Product: TRIM16 Antibody
Catalog: AF0352
Description: Rabbit polyclonal antibody to TRIM16
Application: WB IHC
Reactivity: Human, Mouse, Rat
Mol.Wt.: 63kDa; 64kD(Calculated).
Uniprot: O95361
RRID: AB_2834197

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
IHC 1:50-1:200, WB 1:1000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Clonality:
Polyclonal
Specificity:
TRIM16 Antibody detects endogenous levels of total TRIM16.
RRID:
AB_2834197
Cite Format: Affinity Biosciences Cat# AF0352, RRID:AB_2834197.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

EBBP; Estrogen responsive B box protein; Estrogen-responsive B box protein; TRI16_HUMAN; TRIM 16; TRIM16; Tripartite motif containing 16; Tripartite motif containing protein 16; Tripartite motif-containing protein 16;

Immunogens

Immunogen:
Uniprot:

O95361(TRI16_HUMAN) >>Visit HPA database.

Gene(ID):
TRIM16(10626)
Expression:
O95361 TRI16_HUMAN:

Highest levels found in testis, ovary, small intestine, colon, placenta, heart, skeletal muscle and mammary gland. More highly expressed in the fetus than in the corresponding adult tissues. Expressed in basal keratinocytes.

Description:
May play a role in the regulation of keratinocyte differentiation
Sequence:
MAELDLMAPGPLPRATAQPPAPLSPDSGSPSPDSGSASPVEEEDVGSSEKLGRETEEQDSDSAEQGDPAGEGKEVLCDFCLDDTRRVKAVKSCLTCMVNYCEEHLQPHQVNIKLQSHLLTEPVKDHNWRYCPAHHSPLSAFCCPDQQCICQDCCQEHSGHTIVSLDAARRDKEAELQCTQLDLERKLKLNENAISRLQANQKSVLVSVSEVKAVAEMQFGELLAAVRKAQANVMLFLEEKEQAALSQANGIKAHLEYRSAEMEKSKQELERMAAISNTVQFLEEYCKFKNTEDITFPSVYVGLKDKLSGIRKVITESTVHLIQLLENYKKKLQEFSKEEEYDIRTQVSAVVQRKYWTSKPEPSTREQFLQYAYDITFDPDTAHKYLRLQEENRKVTNTTPWEHPYPDLPSRFLHWRQVLSQQSLYLHRYYFEVEIFGAGTYVGLTCKGIDRKGEERNSCISGNNFSWSLQWNGKEFTAWYSDMETPLKAGPFRRLGVYIDFPGGILSFYGVEYDTMTLVHKFACKFSEPVYAAFWLSKKENAIRIVDLGEEPEKPAPSLVGTAP

PTMs - O95361 As Substrate

Site PTM Type Enzyme
S24 Phosphorylation
S29 Phosphorylation
S31 Phosphorylation
S38 Phosphorylation
K50 Ubiquitination
T55 Phosphorylation
S60 Phosphorylation
S62 Phosphorylation
S116 Phosphorylation
K172 Ubiquitination
K188 Methylation
K188 Ubiquitination
K202 Ubiquitination
S203 Phosphorylation
K240 Ubiquitination
K252 Ubiquitination
K264 Acetylation
K266 Acetylation
K289 Ubiquitination
K304 Ubiquitination
K306 Ubiquitination
K329 Ubiquitination
K331 Ubiquitination
K337 Ubiquitination
Y341 Phosphorylation
K354 Ubiquitination
K359 Ubiquitination
K384 Ubiquitination
Y429 Phosphorylation
Y430 Phosphorylation
Y441 Phosphorylation
Y531 Phosphorylation
S537 Phosphorylation
K554 Ubiquitination

Research Backgrounds

Function:

E3 ubiquitin ligase that plays an essential role in the organization of autophagic response and ubiquitination upon lysosomal and phagosomal damages. Plays a role in the stress-induced biogenesis and degradation of protein aggresomes by regulating the p62-KEAP1-NRF2 signaling and particularly by modulating the ubiquitination levels and thus stability of NRF2. Acts as a scaffold protein and facilitates autophagic degradation of protein aggregates by interacting with p62/SQSTM, ATG16L1 and LC3B/MAP1LC3B. In turn, protects the cell against oxidative stress-induced cell death as a consequence of endomembrane damage.

PTMs:

Phosphorylated by ULK1.

Auto-ubiquitinates via its B-Boxes.

Subcellular Location:

Cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Highest levels found in testis, ovary, small intestine, colon, placenta, heart, skeletal muscle and mammary gland. More highly expressed in the fetus than in the corresponding adult tissues. Expressed in basal keratinocytes.

Subunit Structure:

Homodimerizes via its coiled-coil domain. Heterodimerizes with MID1, TRIM24 and PML. Interacts with Galectin-3/LGALS3 in a ULK1-dependent manner; this interaction mediates autophagy of damage endomembranes. Interacts with BECN1. Interacts with ATG16L1. Interacts with p62/SQSTM and LC3B/MAP1LC3B.

Family&Domains:

Belongs to the TRIM/RBCC family.

References

1). TFEB ameliorates DEHP-induced neurotoxicity by activating GAL3/TRIM16 axis dependent lysophagy and alleviating lysosomal dysfunction. Environmental toxicology, 2024 (PubMed: 38488668) [IF=4.4]
2). TRIM16 mitigates impaired osteogenic differentiation and antioxidant response in D-galactose-induced senescent osteoblasts. European journal of pharmacology, 2024 (PubMed: 39059569) [IF=4.2]
3). Tripartite motif-containing protein 16 protects against myocardial ischemia/reperfusion injury by affecting the Keap1/Nrf2 axis. CELL AND TISSUE RESEARCH, 2021 (PubMed: 34436665) [IF=3.2]

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