FOXL1 Antibody | Affinity Biosciences

WB
IF/ICC

1/2

Western blot analysis on COS7 cell lysates using FOXL1 Antibody,The lane on the left was treated with the antigen-specific peptide.

AF0705 staining Hela cells by IF/ICC.

Product:	FOXL1 Antibody
Catalog:	AF0705
Description:	Rabbit polyclonal antibody to FOXL1
Application:	WB IF/ICC
Reactivity:	Human, Monkey
Prediction:	Bovine, Sheep, Dog
Mol.Wt.:	36kDa; 36kD(Calculated).
Uniprot:	Q12952
RRID:	AB_2834248

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Monkey

Prediction:

Bovine(82%), Sheep(82%), Dog(80%)

Clonality:

Polyclonal

Specificity:

FOXL1 Antibody detects endogenous levels of total FOXL1.

RRID:

AB_2834248
Cite Format: Affinity Biosciences Cat# AF0705, RRID:AB_2834248.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

FKH 6; FKH6; FKHL 11; FKHL11; Forkhead (Drosophila) like 11; Forkhead box L1; Forkhead box protein L1; Forkhead related protein FKHL11; Forkhead related transcription factor 7; Forkhead-related protein FKHL11; Forkhead-related transcription factor 7; FOX L1; FOXL 1; Foxl1; FOXL1_HUMAN; FREAC 7; FREAC-7; FREAC7;

Immunogens

Immunogen:

Uniprot:

Q12952(FOXL1_HUMAN) >>Visit HPA database.

Gene(ID):

FOXL1(2300)

Description:

FOXL1 Transcription factor required for proper proliferation and differentiation in the gastrointestinal epithelium. Target gene of the hedgehog (Hh) signaling pathway via GLI2 AND GLI3 transcription factors.

Sequence:

Q12952 FOXL1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MSHLFDPRLPALAASPMLYLYGPERPGLPLAFAPAAALAASGRAETPQKPPYSYIALIAMAIQDAPEQRVTLNGIYQFIMDRFPFYHDNRQGWQNSIRHNLSLNDCFVKVPREKGRPGKGSYWTLDPRCLDMFENGNYRRRKRKPKPGPGAPEAKRPRAETHQRSAEAQPEAGSGAGGSGPAISRLQAAPAGPSPLLDGPSPPAPLHWPGTASPNEDAGDAAQGAAAVAVGQAARTGDGPGSPLRPASRSSPKSSDKSKSFSIDSILAGKQGQKPPSGDELLGGAKPGPGGRLGASLLAASSSLRPPFNASLMLDPHVQGGFYQLGIPFLSYFPLQVPDTVLHFQ

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Bovine

Sheep

Dog

Pig

Horse

Xenopus

Zebrafish

Chicken

Rabbit

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q12952 As Substrate

Q12952

Site	PTM Type	Enzyme	Source
S96	Phosphorylation		Uniprot
S242	Phosphorylation		Uniprot

Research Backgrounds

Q12952_FOXL1_HUMAN

Function:

Transcription factor required for proper proliferation and differentiation in the gastrointestinal epithelium. Target gene of the hedgehog (Hh) signaling pathway via GLI2 AND GLI3 transcription factors (By similarity).

Subcellular Location:

Nucleus.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.

FOXL1 Antibody - #AF0705