FAKD1 Antibody - #AF0632

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Product: FAKD1 Antibody
Catalog: AF0632
Description: Rabbit polyclonal antibody to FAKD1
Application: WB IF/ICC
Reactivity: Human, Mouse, Monkey
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
Mol.Wt.: 97kDa; 97kD(Calculated).
Uniprot: Q53R41
RRID: AB_2834277

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Monkey
Prediction:
Pig(100%), Bovine(91%), Horse(91%), Sheep(82%), Rabbit(82%), Dog(82%), Xenopus(86%)
Clonality:
Polyclonal
Specificity:
FAKD1 Antibody detects endogenous levels of total FAKD1.
RRID:
AB_2834277
Cite Format: Affinity Biosciences Cat# AF0632, RRID:AB_2834277.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

FAKD1_HUMAN; FAST kinase domain containing protein 1; FAST kinase domain-containing protein 1; FAST kinase domains 1; FASTKD1; FLJ21901; KIAA1800; OTTHUMP00000207008; OTTHUMP00000207010;

Immunogens

Immunogen:
Uniprot:

Q53R41(FAKD1_HUMAN) >>Visit HPA database.

Gene(ID):
FASTKD1(79675)
Expression:
Q53R41 FAKD1_HUMAN:

Expression detected in spleen, thymus, testis, ovary, colon, heart, smooth muscle, kidney, brain, lung, liver and white adipose tissue with highest expression in heart.

Description:
FASTKD1 Belongs to the FAST kinase family. 2 isoforms of the human protein are produced by alternative splicing. Note: This description may include information from UniProtKB.
Sequence:
MKKTPVFLESLVTNMLRLRAICPFSWRVFQFRPISCEPLIIQMNKCTDEEQMFGFIERNKAILSEKQVGCAFDMLWKLQKQKTSLLKNAEYVRDHPQFLTLHNLATNKFKLMNDDTLVNVLYVTQQFAGEAHDPLVEALVTEAWRRLERFDIKLLSEFSSCLADQHLYFSPLMGKIADIVHRNLETTQDLSSLSVLMVNISSLISRHFQQQLVNKTELLFDTIDSSEVNVAKSIAKFLRNVRYRYQPLLERCNNVFLSNVDHLDLDSISKILSVYKFLQFNSFEFIIMAKKKLTEMIPLCNHPASFVKLFVALGPIAGPEEKKQLKSTMLLMSEDLTGEQALAVLGAMGDMESRNSCLIKRVTSVLHKHLDGYKPLELLKITQELTFLHFQRKEFFAKLRELLLSYLKNSFIPTEVSVLVRAISLLPSPHLDEVGISRIEAVLPQCDLNNLSSFATSVLRWIQHDHMYLDNMTAKQLKLLQKLDHYGRQRLQHSNSLDLLRKELKSLKGNTFPESLLEEMIATLQHFMDDINYINVGEIASFISSTDYLSTLLLDRIASVAVQQIEKIHPFTIPAIIRPFSVLNYDPPQRDEFLGTCVQHLNSYLGILDPFILVFLGFSLATLEYFPEDLLKAIFNIKFLARLDSQLEILSPSRSARVQFHLMELNRSVCLECPEFQIPWFHDRFCQQYNKGIGGMDGTQQQIFKMLAEVLGGINCVKASVLTPYYHKVDFECILDKRKKPLPYGSHNIALGQLPEMPWESNIEIVGSRLPPGAERIALEFLDSKALCRNIPHMKGKSAMKKRHLEILGYRVIQISQFEWNSMALSTKDARMDYLRECIFGEVKSCL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
91
Bovine
91
Xenopus
86
Sheep
82
Dog
82
Rabbit
82
Chicken
73
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q53R41 As Substrate

Site PTM Type Enzyme
K60 Ubiquitination
T83 Phosphorylation
K87 Ubiquitination
Y122 Phosphorylation
T124 Phosphorylation
Y168 Phosphorylation
S170 Phosphorylation
S194 Phosphorylation
S202 Phosphorylation
S305 Phosphorylation
K360 Acetylation
K374 Ubiquitination
S405 Phosphorylation
Y406 Phosphorylation
K478 Ubiquitination
K482 Ubiquitination
Y486 Phosphorylation
S496 Phosphorylation
T546 Phosphorylation
T551 Phosphorylation
K691 Ubiquitination
Y726 Phosphorylation
K728 Ubiquitination
K737 Ubiquitination
K785 Ubiquitination
Y810 Phosphorylation
K844 Ubiquitination

Research Backgrounds

Function:

Involved in the down-regulation of mitochondrial MT-ND3 mRNA levels which leads to decreased respiratory complex I abundance and activity.

Subcellular Location:

Mitochondrion.
Note: Preferentially localizes to mitochondrial RNA granules, platforms for post-transcriptional RNA modification and ribosome assembly (PubMed:28335001).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expression detected in spleen, thymus, testis, ovary, colon, heart, smooth muscle, kidney, brain, lung, liver and white adipose tissue with highest expression in heart.

Family&Domains:

The RAP domain is essential to regulate MT-ND3 mRNA levels.

Belongs to the FAST kinase family.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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