Product: STEA3 Antibody
Catalog: AF0652
Description: Rabbit polyclonal antibody to STEA3
Application: WB IHC IF/ICC
Cited expt.: WB
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 54kDa; 55kD(Calculated).
Uniprot: Q658P3
RRID: AB_2834284

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Product Info

Source:
Rabbit
Application:
IHC 1:50-1:200, WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Zebrafish(80%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(90%), Chicken(90%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
STEA3 Antibody detects endogenous levels of total STEA3.
RRID:
AB_2834284
Cite Format: Affinity Biosciences Cat# AF0652, RRID:AB_2834284.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

1010001D01Rik; Dudlin 2; Dudlin2; Dudulin 2; Dudulin-2; Dudulin2; hpHyde; hTSAP6; Metalloreductase STEAP3; MGC93147; pHyde; Six transmembrane epithelial antigen of prostate 3; Six transmembrane prostate protein 3; Six-transmembrane epithelial antigen of prostate 3; STEA 3; STEA3_HUMAN; STEAP 3; STEAP family member 3; STEAP family member 3 metalloreductase; STEAP3; STMP 3; STMP3; TSAP 6; TSAP6; Tumor suppressor activated pathway protein 6; Tumor suppressor pHyde; Tumor suppressor-activated pathway protein 6;

Immunogens

Immunogen:

A synthesized peptide derived from human STEA3, corresponding to a region within N-terminal amino acids.

Uniprot:
Gene(ID):
Expression:
Q658P3 STEA3_HUMAN:

Expressed in adult bone marrow, placenta, liver, skeletal muscle and pancreas. Down-regulated in hepatocellular carcinoma.

Description:
STEAP3 a multi-pass membrane protein induced directly by p53. Plays an important role in facilitating exosome secretion. Enhances secretion in the DNA damage-induced p53-dependent exosomal secretory pathway. Localizes to vesicular-like structures at the plasma membrane and around the nucleus. Highly expressed in hematopoietic tissues where it localizes to the specialized endosome. An endosomal ferrireductase required for efficient transferrin-dependent iron uptake in erythroid cells. Participates in erythroid iron homeostasis by reducing Fe(3+) to Fe(2+). Can also reduce of Cu(2+) to Cu(1+), suggesting that it participates in copper homeostasis. Uses NAD(+) as acceptor.
Sequence:
MPEEMDKPLISLHLVDSDSSLAKVPDEAPKVGILGSGDFARSLATRLVGSGFKVVVGSRNPKRTARLFPSAAQVTFQEEAVSSPEVIFVAVFREHYSSLCSLSDQLAGKILVDVSNPTEQEHLQHRESNAEYLASLFPTCTVVKAFNVISAWTLQAGPRDGNRQVPICGDQPEAKRAVSEMALAMGFMPVDMGSLASAWEVEAMPLRLLPAWKVPTLLALGLFVCFYAYNFVRDVLQPYVQESQNKFFKLPVSVVNTTLPCVAYVLLSLVYLPGVLAAALQLRRGTKYQRFPDWLDHWLQHRKQIGLLSFFCAALHALYSFCLPLRRAHRYDLVNLAVKQVLANKSHLWVEEEVWRMEIYLSLGVLALGTLSLLAVTSLPSIANSLNWREFSFVQSSLGFVALVLSTLHTLTYGWTRAFEESRYKFYLPPTFTLTLLVPCVVILAKALFLLPCISRRLARIRRGWERESTIKFTLPTDHALAEKTSHV

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Xenopus
100
Rabbit
100
Dog
90
Chicken
90
Zebrafish
80
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

Research Backgrounds

Function:

Endosomal ferrireductase required for efficient transferrin-dependent iron uptake in erythroid cells. Participates in erythroid iron homeostasis by reducing Fe(3+) to Fe(2+). Can also reduce of Cu(2+) to Cu(1+), suggesting that it participates in copper homeostasis. Uses NADP(+) as acceptor. May play a role downstream of p53/TP53 to interface apoptosis and cell cycle progression. Indirectly involved in exosome secretion by facilitating the secretion of proteins such as TCTP.

PTMs:

Proteolytically cleaved by RHBDL4/RHBDD1. RHBDL4/RHBDD1-induced cleavage occurs at multiple sites in a glycosylation-independent manner.

Glycosylated.

Subcellular Location:

Endosome membrane>Multi-pass membrane protein.
Note: Localizes to vesicular-like structures at the plasma membrane and around the nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in adult bone marrow, placenta, liver, skeletal muscle and pancreas. Down-regulated in hepatocellular carcinoma.

Family&Domains:

Belongs to the STEAP family.

Research Fields

· Cellular Processes > Cell growth and death > p53 signaling pathway.   (View pathway)

· Cellular Processes > Cell growth and death > Ferroptosis.   (View pathway)

References

1). Internalized polystyrene nanoplastics trigger testicular damage and promote ferroptosis via CISD1 downregulation in mouse spermatocyte. JOURNAL OF NANOBIOTECHNOLOGY, 2025 [IF=12.6]

Application: WB    Species: Mouse    Sample: GC-2 cells

Fig. 3 PS-NPs initiate ferroptosis by triggering iron overload and ROS overproduction in GC-2 cells. (A) Various concentrations of PS-NPs (50 µg/mL, 100 µg/mL, 200 µg/mL) in different treatment times (6–24 h) reduce cell viability in GC-2 cells. (B and C) Representative images and quantification of fluorescence intensity for lipid peroxidation levels labelled with C11 BODIPY in GC-2 cells post-exposed with PS-NPs. Statistical analysis presented the ratio of MFI of green to red. (D-F) The levels of MDA, GSH, and total Fe were detected in GC-2 cells after PS-NPs treatment for 12 h. (G and H) Representative images and quantification of fluorescence intensity showing intracellular chelatable iron in GC-2 cells post-exposure to PS-NPs labelled with FerroOrange (red). (I and J) DCFH-DA staining was performed to detect ROS levels of GC-2 cells after PS-NPs exposure for 12 h using fluorescent microscopy. (K) Flow cytometry analysis of and ROS levels of GC-2 cells after PS-NPs exposure for 12 h using Fluorescence-activated cell sorting (FACS). (L) qRT-PCR analysis of ferroptosis-related gene expression in GC-2 cells treated with or without PS-NPs. (M and N) Ferroptosis-related proteins in GC-2 cells stimulated by PS-NPs were determined by western blotting. And semi-quantification of protein expression levels normalized to β-actin. (O) Representative TEM images depicting mitochondria in GC-2 cells in response to exposure to PS-NPs. The reduction of mitochondrial cristae is denoted by yellow arrows, while red arrows signify mitochondrial membrane rupture. (P and Q) Representative illustration and quantification of fluorescence intensity depicting mitochondria in GC-2 cells after treatment with PS-NPs using Mito-Tracker Green. (R and S) Illustration and quantification data of mitochondrial membrane potential in GC-2 cells in response to treatment of PS-NPs stained with TMRE

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