Phospho-Huntingtin (Ser421)[Ser419] Antibody - #AF8067

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Product: Phospho-Huntingtin (Ser421)[Ser419] Antibody
Catalog: AF8067
Description: Rabbit polyclonal antibody to Phospho-Huntingtin (Ser421)[Ser419]
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.: 348kDa; 348kD(Calculated).
Uniprot: P42858
RRID: AB_2840130

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 100ul $350 In stock
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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(89%), Dog(89%), Chicken(100%)
Clonality:
Polyclonal
Specificity:
Phospho-Huntingtin (Ser421) Antibody detects endogenous levels of Huntingtin only when phosphorylated at Ser419, which site historically referenced as Ser421.
RRID:
AB_2840130
Cite Format: Affinity Biosciences Cat# AF8067, RRID:AB_2840130.
Conjugate:
Unconjugated.
Purification:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

AI256365; C430023I11Rik; HD; HD protein; HD_HUMAN; HDH; HTT; Huntingtin; HUNTINGTON CHOREA; Huntington disease protein; Huntington's disease protein homolog; IT 15; IT15; OTTMUSP00000026909; ZHD;

Immunogens

Immunogen:
Uniprot:

P42858(HD_HUMAN) >>Visit HPA database.

Gene(ID):
HTT(3064)
Expression:
P42858 HD_HUMAN:

Expressed in the brain cortex (at protein level). Widely expressed with the highest level of expression in the brain (nerve fibers, varicosities, and nerve endings). In the brain, the regions where it can be mainly found are the cerebellar cortex, the neocortex, the striatum, and the hippocampal formation.

Sequence:
MATLEKLMKAFESLKSFQQQQQQQQQQQQQQQQQQQQQPPPPPPPPPPPQLPQPPPQAQPLLPQPQPPPPPPPPPPGPAVAEEPLHRPKKELSATKKDRVNHCLTICENIVAQSVRNSPEFQKLLGIAMELFLLCSDDAESDVRMVADECLNKVIKALMDSNLPRLQLELYKEIKKNGAPRSLRAALWRFAELAHLVRPQKCRPYLVNLLPCLTRTSKRPEESVQETLAAAVPKIMASFGNFANDNEIKVLLKAFIANLKSSSPTIRRTAAGSAVSICQHSRRTQYFYSWLLNVLLGLLVPVEDEHSTLLILGVLLTLRYLVPLLQQQVKDTSLKGSFGVTRKEMEVSPSAEQLVQVYELTLHHTQHQDHNVVTGALELLQQLFRTPPPELLQTLTAVGGIGQLTAAKEESGGRSRSGSIVELIAGGGSSCSPVLSRKQKGKVLLGEEEALEDDSESRSDVSSSALTASVKDEISGELAASSGVSTPGSAGHDIITEQPRSQHTLQADSVDLASCDLTSSATDGDEEDILSHSSSQVSAVPSDPAMDLNDGTQASSPISDSSQTTTEGPDSAVTPSDSSEIVLDGTDNQYLGLQIGQPQDEDEEATGILPDEASEAFRNSSMALQQAHLLKNMSHCRQPSDSSVDKFVLRDEATEPGDQENKPCRIKGDIGQSTDDDSAPLVHCVRLLSASFLLTGGKNVLVPDRDVRVSVKALALSCVGAAVALHPESFFSKLYKVPLDTTEYPEEQYVSDILNYIDHGDPQVRGATAILCGTLICSILSRSRFHVGDWMGTIRTLTGNTFSLADCIPLLRKTLKDESSVTCKLACTAVRNCVMSLCSSSYSELGLQLIIDVLTLRNSSYWLVRTELLETLAEIDFRLVSFLEAKAENLHRGAHHYTGLLKLQERVLNNVVIHLLGDEDPRVRHVAAASLIRLVPKLFYKCDQGQADPVVAVARDQSSVYLKLLMHETQPPSHFSVSTITRIYRGYNLLPSITDVTMENNLSRVIAAVSHELITSTTRALTFGCCEALCLLSTAFPVCIWSLGWHCGVPPLSASDESRKSCTVGMATMILTLLSSAWFPLDLSAHQDALILAGNLLAASAPKSLRSSWASEEEANPAATKQEEVWPALGDRALVPMVEQLFSHLLKVINICAHVLDDVAPGPAIKAALPSLTNPPSLSPIRRKGKEKEPGEQASVPLSPKKGSEASAASRQSDTSGPVTTSKSSSLGSFYHLPSYLKLHDVLKATHANYKVTLDLQNSTEKFGGFLRSALDVLSQILELATLQDIGKCVEEILGYLKSCFSREPMMATVCVQQLLKTLFGTNLASQFDGLSSNPSKSQGRAQRLGSSSVRPGLYHYCFMAPYTHFTQALADASLRNMVQAEQENDTSGWFDVLQKVSTQLKTNLTSVTKNRADKNAIHNHIRLFEPLVIKALKQYTTTTCVQLQKQVLDLLAQLVQLRVNYCLLDSDQVFIGFVLKQFEYIEVGQFRESEAIIPNIFFFLVLLSYERYHSKQIIGIPKIIQLCDGIMASGRKAVTHAIPALQPIVHDLFVLRGTNKADAGKELETQKEVVVSMLLRLIQYHQVLEMFILVLQQCHKENEDKWKRLSRQIADIILPMLAKQQMHIDSHEALGVLNTLFEILAPSSLRPVDMLLRSMFVTPNTMASVSTVQLWISGILAILRVLISQSTEDIVLSRIQELSFSPYLISCTVINRLRDGDSTSTLEEHSEGKQIKNLPEETFSRFLLQLVGILLEDIVTKQLKVEMSEQQHTFYCQELGTLLMCLIHIFKSGMFRRITAAATRLFRSDGCGGSFYTLDSLNLRARSMITTHPALVLLWCQILLLVNHTDYRWWAEVQQTPKRHSLSSTKLLSPQMSGEEEDSDLAAKLGMCNREIVRRGALILFCDYVCQNLHDSEHLTWLIVNHIQDLISLSHEPPVQDFISAVHRNSAASGLFIQAIQSRCENLSTPTMLKKTLQCLEGIHLSQSGAVLTLYVDRLLCTPFRVLARMVDILACRRVEMLLAANLQSSMAQLPMEELNRIQEYLQSSGLAQRHQRLYSLLDRFRLSTMQDSLSPSPPVSSHPLDGDGHVSLETVSPDKDWYVHLVKSQCWTRSDSALLEGAELVNRIPAEDMNAFMMNSEFNLSLLAPCLSLGMSEISGGQKSALFEAAREVTLARVSGTVQQLPAVHHVFQPELPAEPAAYWSKLNDLFGDAALYQSLPTLARALAQYLVVVSKLPSHLHLPPEKEKDIVKFVVATLEALSWHLIHEQIPLSLDLQAGLDCCCLALQLPGLWSVVSSTEFVTHACSLIYCVHFILEAVAVQPGEQLLSPERRTNTPKAISEEEEEVDPNTQNPKYITAACEMVAEMVESLQSVLALGHKRNSGVPAFLTPLLRNIIISLARLPLVNSYTRVPPLVWKLGWSPKPGGDFGTAFPEIPVEFLQEKEVFKEFIYRINTLGWTSRTQFEETWATLLGVLVTQPLVMEQEESPPEEDTERTQINVLAVQAITSLVLSAMTVPVAGNPAVSCLEQQPRNKPLKALDTRFGRKLSIIRGIVEQEIQAMVSKRENIATHHLYQAWDPVPSLSPATTGALISHEKLLLQINPERELGSMSYKLGQVSIHSVWLGNSITPLREEEWDEEEEEEADAPAPSSPPTSPVNSRKHRAGVDIHSCSQFLLELYSRWILPSSSARRTPAILISEVVRSLLVVSDLFTERNQFELMYVTLTELRRVHPSEDEILAQYLVPATCKAAAVLGMDKAVAEPVSRLLESTLRSSHLPSRVGALHGVLYVLECDLLDDTAKQLIPVISDYLLSNLKGIAHCVNIHSQQHVLVMCATAFYLIENYPLDVGPEFSASIIQMCGVMLSGSEESTPSIIYHCALRGLERLLLSEQLSRLDAESLVKLSVDRVNVHSPHRAMAALGLMLTCMYTGKEKVSPGRTSDPNPAAPDSESVIVAMERVSVLFDRIRKGFPCEARVVARILPQFLDDFFPPQDIMNKVIGEFLSNQQPYPQFMATVVYKVFQTLHSTGQSSMVRDWVMLSLSNFTQRAPVAMATWSLSCFFVSASTSPWVAAILPHVISRMGKLEQVDVNLFCLVATDFYRHQIEEELDRRAFQSVLEVVAAPGSPYHRLLTCLRNVHKVTTC

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Chicken
100
Dog
89
Rabbit
89
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P42858 As Substrate

Site PTM Type Enzyme
T3 Phosphorylation
K6 Acetylation
K6 Sumoylation
K9 Acetylation
K9 Sumoylation
S13 Phosphorylation
K15 Acetylation
K15 Sumoylation
S16 Phosphorylation
K96 Acetylation
S114 Phosphorylation
S118 Phosphorylation
K176 Acetylation
K234 Acetylation
K253 Ubiquitination
T269 Phosphorylation
S276 Phosphorylation
C278 S-Nitrosylation
K335 Ubiquitination
S337 Phosphorylation
K343 Acetylation
T405 Phosphorylation
S411 Phosphorylation
S415 Phosphorylation
S417 Phosphorylation
S419 Phosphorylation O00141 (SGK1) , P31749 (AKT1)
S429 Phosphorylation
S430 Phosphorylation
S432 Phosphorylation Q00535 (CDK5)
S436 Phosphorylation
K442 Acetylation
K442 Ubiquitination
S455 Phosphorylation
S457 Phosphorylation
S459 Phosphorylation
S462 Phosphorylation
S463 Phosphorylation
S464 Phosphorylation
S485 Phosphorylation
T486 Phosphorylation
S489 Phosphorylation
S534 Phosphorylation
S620 Phosphorylation
S640 Phosphorylation
S642 Phosphorylation
S643 Phosphorylation
T654 Phosphorylation
K662 Ubiquitination
K667 Ubiquitination
K698 Ubiquitination
T796 Phosphorylation
K813 Ubiquitination
K902 Ubiquitination
S930 Phosphorylation
K937 Ubiquitination
K941 Ubiquitination
S1104 Phosphorylation
S1111 Phosphorylation
T1120 Phosphorylation
K1121 Ubiquitination
T1173 Phosphorylation
S1177 Phosphorylation
S1179 Phosphorylation Q00535 (CDK5)
S1195 Phosphorylation
S1199 Phosphorylation Q00535 (CDK5)
S1210 Phosphorylation
T1215 Phosphorylation
K1223 Ubiquitination
S1229 Phosphorylation
K1244 Ubiquitination
K1262 Ubiquitination
T1282 Phosphorylation
T1322 Phosphorylation
K1337 Ubiquitination
S1348 Phosphorylation
S1349 Phosphorylation
Y1355 Phosphorylation
K1402 Ubiquitination
K1410 Ubiquitination
K1415 Ubiquitination
K1431 Ubiquitination
S1805 Phosphorylation
S1811 Phosphorylation
Y1813 Phosphorylation
T1814 Phosphorylation
S1862 Phosphorylation
S1864 Phosphorylation
T1866 Phosphorylation
S1870 Phosphorylation
S1874 Phosphorylation
S1880 Phosphorylation
K1885 Ubiquitination
S2074 Phosphorylation
S2233 Phosphorylation
T2335 Phosphorylation
K2337 Acetylation
S2340 Phosphorylation
S2382 Phosphorylation
K2417 Ubiquitination
K2423 Ubiquitination
K2443 Ubiquitination
K2537 Ubiquitination
S2548 Phosphorylation
K2564 Ubiquitination
S2651 Phosphorylation
T2654 Phosphorylation
S2655 Phosphorylation
S2708 Phosphorylation
T2712 Phosphorylation
K2757 Ubiquitination
S2764 Phosphorylation
S2892 Phosphorylation
K2901 Ubiquitination
S2934 Phosphorylation
T2938 Phosphorylation
S2948 Phosphorylation
S2950 Phosphorylation
K2967 Ubiquitination

Research Backgrounds

Function:

May play a role in microtubule-mediated transport or vesicle function.

Promotes the formation of autophagic vesicles.

PTMs:

Cleaved by caspases downstream of the polyglutamine stretch. The resulting N-terminal fragments are cytotoxic and provokes apoptosis.

Forms with expanded polyglutamine expansion are specifically ubiquitinated by SYVN1, which promotes their proteasomal degradation.

Phosphorylation at Ser-1179 and Ser-1199 by CDK5 in response to DNA damage in nuclei of neurons protects neurons against polyglutamine expansion as well as DNA damage mediated toxicity.

Myristoylated at Gly-551, following proteolytic cleavage at Asp-550.

Subcellular Location:

Cytoplasm. Nucleus. Early endosome.
Note: The mutant Huntingtin protein colocalizes with AKAP8L in the nuclear matrix of Huntington disease neurons. Shuttles between cytoplasm and nucleus in a Ran GTPase-independent manner (PubMed:15654337). Recruits onto early endosomes in a Rab5- and HAP40-dependent fashion (PubMed:16476778).

Cytoplasmic vesicle>Autophagosome.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in the brain cortex (at protein level). Widely expressed with the highest level of expression in the brain (nerve fibers, varicosities, and nerve endings). In the brain, the regions where it can be mainly found are the cerebellar cortex, the neocortex, the striatum, and the hippocampal formation.

Subunit Structure:

Interacts with PFN1. Interacts through its N-terminus with PRPF40A. Interacts with PQBP1. Interacts with SETD2. Interacts with SH3GLB1 (By similarity). Interacts with SYVN. Interacts with TPR; the interaction is inhibited by forms of Huntingtin with expanded polyglutamine stretch. Interacts with ZDHHC13 (via ANK repeats). Interacts with ZDHHC17 (via ANK repeats). Interacts with F8A1/F8A2/F8A3. Found in a complex with F8A1/F8A2/F8A3, HTT and RAB5A; mediates the recruitment of HTT by RAB5A.

Family&Domains:

The N-terminal Gln-rich and Pro-rich domain has great conformational flexibility and is likely to exist in a fluctuating equilibrium of alpha-helical, random coil, and extended conformations.

Belongs to the huntingtin family.

Research Fields

· Human Diseases > Neurodegenerative diseases > Huntington's disease.

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