Phospho-TTK (Thr676) Antibody - #AF8194

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Product: Phospho-TTK (Thr676) Antibody
Catalog: AF8194
Description: Rabbit polyclonal antibody to Phospho-TTK (Thr676)
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.: 100kDa; 97kD(Calculated).
Uniprot: P33981
RRID: AB_2840256

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 100ul $350 In stock
 200ul $450 In stock

Lead Time: Same day delivery

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(82%), Bovine(91%), Horse(91%), Sheep(91%), Rabbit(91%), Dog(91%), Chicken(82%)
Clonality:
Polyclonal
Specificity:
Phospho-TTK (Thr676) Antibody detects endogenous levels of TTK only when phosphorylated at Thr676.
RRID:
AB_2840256
Cite Format: Affinity Biosciences Cat# AF8194, RRID:AB_2840256.
Conjugate:
Unconjugated.
Purification:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

cancer/testis antigen 96; CT96; Dual specificity protein kinase TTK; ECSTY kinase; ESK; FLJ38280; hMPS1; Monopolar Spindle 1 Like 1; MPH1; Mps 1; MPS1L1; Phosphotyrosine Picked Threonine Kinase; Phosphotyrosine picked threonine-protein kinase; PYT; RP3-357D13.2; ttk; TTK Protein Kinase; TTK_HUMAN;

Immunogens

Immunogen:
Uniprot:

P33981(TTK_HUMAN) >>Visit HPA database.

Gene(ID):
TTK(7272)
Expression:
P33981 TTK_HUMAN:

Present in rapidly proliferating cell lines.

Sequence:
MESEDLSGRELTIDSIMNKVRDIKNKFKNEDLTDELSLNKISADTTDNSGTVNQIMMMANNPEDWLSLLLKLEKNSVPLSDALLNKLIGRYSQAIEALPPDKYGQNESFARIQVRFAELKAIQEPDDARDYFQMARANCKKFAFVHISFAQFELSQGNVKKSKQLLQKAVERGAVPLEMLEIALRNLNLQKKQLLSEEEKKNLSASTVLTAQESFSGSLGHLQNRNNSCDSRGQTTKARFLYGENMPPQDAEIGYRNSLRQTNKTKQSCPFGRVPVNLLNSPDCDVKTDDSVVPCFMKRQTSRSECRDLVVPGSKPSGNDSCELRNLKSVQNSHFKEPLVSDEKSSELIITDSITLKNKTESSLLAKLEETKEYQEPEVPESNQKQWQSKRKSECINQNPAASSNHWQIPELARKVNTEQKHTTFEQPVFSVSKQSPPISTSKWFDPKSICKTPSSNTLDDYMSCFRTPVVKNDFPPACQLSTPYGQPACFQQQQHQILATPLQNLQVLASSSANECISVKGRIYSILKQIGSGGSSKVFQVLNEKKQIYAIKYVNLEEADNQTLDSYRNEIAYLNKLQQHSDKIIRLYDYEITDQYIYMVMECGNIDLNSWLKKKKSIDPWERKSYWKNMLEAVHTIHQHGIVHSDLKPANFLIVDGMLKLIDFGIANQMQPDTTSVVKDSQVGTVNYMPPEAIKDMSSSRENGKSKSKISPKSDVWSLGCILYYMTYGKTPFQQIINQISKLHAIIDPNHEIEFPDIPEKDLQDVLKCCLKRDPKQRISIPELLAHPYVQIQTHPVNQMAKGTTEEMKYVLGQLVGLNSPNSILKAAKTLYEHYSGGESHNSSSSKTFEKKRGKK

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
91
Bovine
91
Sheep
91
Dog
91
Rabbit
91
Pig
82
Chicken
82
Xenopus
73
Zebrafish
64
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P33981 As Substrate

Site PTM Type Enzyme
M1 Acetylation
S3 Phosphorylation
S7 Phosphorylation
T12 Phosphorylation P33981 (TTK)
S15 Phosphorylation P33981 (TTK)
K19 Methylation
K19 Ubiquitination
K26 Ubiquitination
K28 Sumoylation
K28 Ubiquitination
T33 Phosphorylation P33981 (TTK)
S37 Phosphorylation P33981 (TTK)
T46 Phosphorylation
K71 Sumoylation
K74 Ubiquitination
S80 Phosphorylation P33981 (TTK)
K86 Ubiquitination
K102 Ubiquitination
S108 Phosphorylation
K120 Ubiquitination
Y131 Phosphorylation
K140 Ubiquitination
K163 Ubiquitination
K168 Ubiquitination
K191 Ubiquitination
K192 Ubiquitination
K201 Ubiquitination
T210 Phosphorylation
S214 Phosphorylation
S216 Phosphorylation
S218 Phosphorylation
Y255 Phosphorylation
S258 Phosphorylation
K266 Ubiquitination
S281 Phosphorylation P15056 (BRAF)
K287 Sumoylation
K287 Ubiquitination
T288 Phosphorylation O96017 (CHEK2) , P33981 (TTK)
S291 Phosphorylation
K315 Ubiquitination
S317 Phosphorylation
S321 Phosphorylation
K328 Ubiquitination
S333 Phosphorylation
K336 Ubiquitination
S345 Phosphorylation
T351 Phosphorylation
S353 Phosphorylation
K359 Ubiquitination
T360 Phosphorylation P33981 (TTK)
S362 Phosphorylation P33981 (TTK)
S363 Phosphorylation P33981 (TTK)
K367 Sumoylation
K367 Ubiquitination
T371 Phosphorylation
K372 Ubiquitination
Y374 Phosphorylation
S382 Phosphorylation
K385 Methylation
K385 Ubiquitination
K392 Ubiquitination
S393 Phosphorylation
K415 Ubiquitination
S433 Phosphorylation
K434 Ubiquitination
S436 Phosphorylation P15056 (BRAF) , P33981 (TTK)
S440 Phosphorylation
K443 Ubiquitination
K448 Ubiquitination
K452 Ubiquitination
T453 Phosphorylation
S455 Phosphorylation
T458 Phosphorylation
Y462 Phosphorylation
S464 Phosphorylation
R467 Methylation
T468 Phosphorylation
K472 Sumoylation
K529 Ubiquitination
K538 Ubiquitination
K546 Ubiquitination
K547 Ubiquitination
K553 Ubiquitination
T564 Phosphorylation P33981 (TTK)
K577 Ubiquitination
S582 Phosphorylation P33981 (TTK)
K584 Ubiquitination
T594 Phosphorylation
K617 Ubiquitination
S618 Phosphorylation
K625 Ubiquitination
K649 Ubiquitination
T675 Phosphorylation P33981 (TTK)
T676 Phosphorylation P33981 (TTK)
S677 Phosphorylation P33981 (TTK)
K680 Ubiquitination
S682 Phosphorylation
T686 Phosphorylation P33981 (TTK)
K696 Ubiquitination
K708 Methylation
S742 Phosphorylation P33981 (TTK)
K762 Ubiquitination
K769 Ubiquitination
T795 Phosphorylation P33981 (TTK)
K803 Ubiquitination
T805 Phosphorylation P33981 (TTK)
T806 Phosphorylation P33981 (TTK)
K810 Ubiquitination
Y811 Phosphorylation P33981 (TTK)
S821 Phosphorylation P28482 (MAPK1) , P27361 (MAPK3) , P33981 (TTK)
S824 Phosphorylation P33981 (TTK)
K827 Ubiquitination
K830 Ubiquitination
Y833 Phosphorylation
Y836 Phosphorylation
S837 Phosphorylation
S845 Phosphorylation P33981 (TTK)
S847 Phosphorylation P33981 (TTK)
K848 Ubiquitination
T849 Phosphorylation P33981 (TTK)
K852 Acetylation
K853 Acetylation

PTMs - P33981 As Enzyme

Substrate Site Source
O60566 (BUB1B) S435 Uniprot
O60566 (BUB1B) S543 Uniprot
O60566 (BUB1B) S670 Uniprot
O60566 (BUB1B) S1043 Uniprot
O96017 (CHEK2) T68 Uniprot
P00519 (ABL1) T735 Uniprot
P04637 (TP53) T18 Uniprot
P07900 (HSP90AA1) T115 Uniprot
P33981 (TTK) T12 Uniprot
P33981 (TTK) S15 Uniprot
P33981 (TTK) T33 Uniprot
P33981 (TTK) S37 Uniprot
P33981 (TTK) S80 Uniprot
P33981 (TTK) T288 Uniprot
P33981 (TTK) T360 Uniprot
P33981 (TTK) S362 Uniprot
P33981 (TTK) S363 Uniprot
P33981 (TTK) S436 Uniprot
P33981 (TTK) T564 Uniprot
P33981 (TTK) S582 Uniprot
P33981 (TTK) T675 Uniprot
P33981 (TTK) T676 Uniprot
P33981 (TTK) S677 Uniprot
P33981 (TTK) T686 Uniprot
P33981 (TTK) S742 Uniprot
P33981 (TTK) T795 Uniprot
P33981 (TTK) T805 Uniprot
P33981 (TTK) T806 Uniprot
P33981 (TTK) Y811 Uniprot
P33981 (TTK) S821 Uniprot
P33981 (TTK) S824 Uniprot
P33981 (TTK) S845 Uniprot
P33981 (TTK) S847 Uniprot
P33981 (TTK) T849 Uniprot
P38646 (HSPA9) T62 Uniprot
P38646 (HSPA9) S65 Uniprot
P54132 (BLM) S144 Uniprot
P54132 (BLM) S1290 Uniprot
P54132 (BLM) S1296 Uniprot
Q53HL2 (CDCA8) T88 Uniprot
Q53HL2 (CDCA8) T94 Uniprot
Q53HL2 (CDCA8) T169 Uniprot
Q53HL2 (CDCA8) T230 Uniprot
Q53HL2 (CDCA8) S238 Uniprot
Q96E14 (RMI2) S112 Uniprot

Research Backgrounds

Function:

Phosphorylates proteins on serine, threonine, and tyrosine. Probably associated with cell proliferation. Essential for chromosome alignment by enhancing AURKB activity (via direct CDCA8 phosphorylation) at the centromere, and for the mitotic checkpoint.

Tissue Specificity:

Present in rapidly proliferating cell lines.

Subunit Structure:

Interacts with TPR; the interactions occurs in a microtubule-independent manner.

Family&Domains:

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.

Research Fields

· Cellular Processes > Cell growth and death > Cell cycle.   (View pathway)

References

1). Design, synthesis and biological evaluation of a new class of 7H-pyrrolo[2,3-d]pyrimidine derivatives as Mps1 inhibitors for the treatment of breast cancer. European Journal of Medicinal Chemistry, 2023 (PubMed: 36370549) [IF=6.0]
2). Discovery of a potent and selective covalent threonine tyrosine kinase (TTK) inhibitor. Bioorganic chemistry, 2024 (PubMed: 38159497) [IF=4.5]

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