EPN2 Antibody - #AF0682
Product: | EPN2 Antibody |
Catalog: | AF0682 |
Description: | Rabbit polyclonal antibody to EPN2 |
Application: | WB IHC IF/ICC |
Reactivity: | Human, Mouse, Rat |
Prediction: | Pig, Bovine, Sheep, Rabbit, Dog |
Mol.Wt.: | 68kDa; 68kD(Calculated). |
Uniprot: | O95208 |
RRID: | AB_2834390 |
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Protocols
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# AF0682, RRID:AB_2834390.
Fold/Unfold
EHB21; EPN 2; Epn2; EPN2_HUMAN; EPS 15 interacting protein 2; EPS-15-interacting protein 2; Eps15 binding protein; Epsin 2; Epsin-2; Epsin2; KIAA1065; OTTHUMP00000065808; OTTHUMP00000065809; OTTHUMP00000065810; OTTHUMP00000065811; OTTHUMP00000065889;
Immunogens
- O95208 EPN2_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MTTSSIRRQMKNIVNNYSEAEIKVREATSNDPWGPSSSLMTEIADLTYNVVAFSEIMSMVWKRLNDHGKNWRHVYKALTLLDYLIKTGSERVAQQCRENIFAIQTLKDFQYIDRDGKDQGINVREKSKQLVALLKDEERLKAERAQALKTKERMAQVATGMGSNQITFGRGSSQPNLSTSHSEQEYGKAGGSPASYHGSPEASLCPQHRTGAPLGQSEELQPLSQRHPFLPHLGLASRPNGDWSQPCLTCDRAARATSPRVSSELEQARPQTSGEEELQLQLALAMSREVAEQEERLRRGDDLRLQMALEESRRDTVKIPKKKEHGSLPQQTTLLDLMDALPSSGPAAQKAEPWGPSASTNQTNPWGGPAAPASTSDPWPSFGTKPAASIDPWGVPTGATVQSVPKNSDPWAASQQPASSAGKRASDAWGAVSTTKPVSVSGSFELFSNLNGTIKDDFSEFDNLRTSKKTAESVTSLPSQNNGTTSPDPFESQPLTVASSKPSSARKTPESFLGPNAALVNLDSLVTRPAPPAQSLNPFLAPGAPATSAPVNPFQVNQPQPLTLNQLRGSPVLGTSTSFGPGPGVESMAVASMTSAAPQPALGATGSSLTPLGPAMMNMVGSVGIPPSAAQATGTTNPFLL
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - O95208 As Substrate
Site | PTM Type | Enzyme | Source |
---|---|---|---|
T2 | Phosphorylation | Uniprot | |
S4 | Phosphorylation | Uniprot | |
S5 | Phosphorylation | Uniprot | |
K11 | Ubiquitination | Uniprot | |
K69 | Ubiquitination | Uniprot | |
Y83 | Phosphorylation | Uniprot | |
K107 | Ubiquitination | Uniprot | |
K117 | Ubiquitination | Uniprot | |
K128 | Ubiquitination | Uniprot | |
K135 | Ubiquitination | Uniprot | |
K141 | Ubiquitination | Uniprot | |
T150 | Phosphorylation | Uniprot | |
S163 | Phosphorylation | Uniprot | |
R170 | Methylation | Uniprot | |
S172 | Phosphorylation | Uniprot | |
S173 | Phosphorylation | Uniprot | |
S178 | Phosphorylation | Uniprot | |
S182 | Phosphorylation | Uniprot | |
Y186 | Phosphorylation | Uniprot | |
S192 | Phosphorylation | Uniprot | |
S195 | Phosphorylation | Uniprot | |
S199 | Phosphorylation | Uniprot | |
S237 | Phosphorylation | Uniprot | |
T249 | Phosphorylation | Uniprot | |
T272 | Phosphorylation | Uniprot | |
S273 | Phosphorylation | Uniprot | |
S287 | Phosphorylation | Uniprot | |
T316 | Phosphorylation | Uniprot | |
K318 | Ubiquitination | Uniprot | |
S327 | Phosphorylation | Uniprot | |
S408 | Phosphorylation | Uniprot | |
S420 | Phosphorylation | Uniprot | |
K423 | Acetylation | Uniprot | |
K423 | Methylation | Uniprot | |
K423 | Ubiquitination | Uniprot | |
S426 | Phosphorylation | Uniprot | |
S486 | Phosphorylation | Uniprot | |
S492 | Phosphorylation | Uniprot | |
S504 | Phosphorylation | Uniprot | |
T508 | Phosphorylation | Uniprot | |
S570 | Phosphorylation | Uniprot | |
S622 | Phosphorylation | Uniprot | |
S628 | Phosphorylation | Uniprot |
Research Backgrounds
Plays a role in the formation of clathrin-coated invaginations and endocytosis.
Ubiquitinated.
Cytoplasm. Cytoplasmic vesicle>Clathrin-coated vesicle.
Note: In punctate structures throughout the cell, associated with clathrin-coated vesicles, and particularly concentrated in the region of the Golgi complex.
Highest expression is found in brain. Detected at lower levels in lung and liver.
Binds EPS15 (By similarity). Interacts with ITSN1 (By similarity). Binds AP-2 and clathrin. Interacts with UBQLN2.
The NPF repeat domain is involved in EPS15 binding.
The DPW repeat domain is involved in AP-2 and clathrin binding.
Belongs to the epsin family.
Research Fields
· Cellular Processes > Transport and catabolism > Endocytosis. (View pathway)
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