IHH Antibody - #DF7688

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Product: IHH Antibody
Catalog: DF7688
Description: Rabbit polyclonal antibody to IHH
Application: WB IF/ICC
Reactivity: Human, Mouse, Rat, Monkey
Prediction: Zebrafish, Sheep, Rabbit, Chicken, Xenopus
Mol.Wt.: 45 kDa; 45kD(Calculated).
Uniprot: Q14623
RRID: AB_2841159

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat,Monkey
Prediction:
Zebrafish(92%), Sheep(100%), Rabbit(100%), Chicken(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
IHH Antibody detects endogenous levels of total IHH.
RRID:
AB_2841159
Cite Format: Affinity Biosciences Cat# DF7688, RRID:AB_2841159.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

BDA1; HHG-2; HHG2; IHH; IHH_HUMAN; Indian Hedgehog; Indian hedgehog homolog; Indian hedgehog protein; Indian hedgehog protein C-product; Indian hedgehog protein N-product;

Immunogens

Immunogen:
Uniprot:

Q14623(IHH_HUMAN) >>Visit HPA database.

Gene(ID):
IHH(3549)
Expression:
Q14623 IHH_HUMAN:

Expressed in embryonic lung, and in adult kidney and liver.

Sequence:
MSPARLRPRLHFCLVLLLLLVVPAAWGCGPGRVVGSRRRPPRKLVPLAYKQFSPNVPEKTLGASGRYEGKIARSSERFKELTPNYNPDIIFKDEENTGADRLMTQRCKDRLNSLAISVMNQWPGVKLRVTEGWDEDGHHSEESLHYEGRAVDITTSDRDRNKYGLLARLAVEAGFDWVYYESKAHVHCSVKSEHSAAAKTGGCFPAGAQVRLESGARVALSAVRPGDRVLAMGEDGSPTFSDVLIFLDREPHRLRAFQVIETQDPPRRLALTPAHLLFTADNHTEPAARFRATFASHVQPGQYVLVAGVPGLQPARVAAVSTHVALGAYAPLTKHGTLVVEDVVASCFAAVADHHLAQLAFWPLRLFHSLAWGSWTPGEGVHWYPQLLYRLGRLLLEEGSFHPLGMSGAGS

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Sheep
100
Xenopus
100
Chicken
100
Rabbit
100
Zebrafish
92
Pig
0
Horse
0
Bovine
0
Dog
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q14623 As Substrate

Site PTM Type Enzyme
N282 N-Glycosylation
T293 Phosphorylation
S296 Phosphorylation
Y303 Phosphorylation
S400 Phosphorylation
S407 Phosphorylation

Research Backgrounds

Function:

Intercellular signal essential for a variety of patterning events during development. Binds to the patched (PTC) receptor, which functions in association with smoothened (SMO), to activate the transcription of target genes. Implicated in endochondral ossification: may regulate the balance between growth and ossification of the developing bones. Induces the expression of parathyroid hormone-related protein (PTHRP) (By similarity).

PTMs:

The C-terminal domain displays an autoproteolysis activity and a cholesterol transferase activity. Both activities result in the cleavage of the full-length protein and covalent attachment of a cholesterol moiety to the C-terminal of the newly generated N-terminal fragment (N-product). The N-product is the active species in both local and long-range signaling, whereas the C-product has no signaling activity (By similarity).

Cholesterylation is required for N-product targeting to lipid rafts and multimerization.

Palmitoylated. N-palmitoylation is required for N-product multimerization and full activity.

Subcellular Location:

Cell membrane>Lipid-anchor>Extracellular side.
Note: The N-terminal peptide remains associated with the cell surface.

Secreted>Extracellular space.
Note: The C-terminal peptide diffuses from the cell.

Cell membrane.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in embryonic lung, and in adult kidney and liver.

Subunit Structure:

Homooligomer (indian hedgehog protein N-product). Interacts with BOC and CDON. Interacts with PTCH1. Interacts with glypican GPC3 (By similarity).

Family&Domains:

The indian hedgehog protein N-product binds calcium and zinc ions; this stabilizes the protein fold and is essential for protein-protein interactions mediated by this domain.

Belongs to the hedgehog family.

Research Fields

· Environmental Information Processing > Signal transduction > Hedgehog signaling pathway.   (View pathway)

· Human Diseases > Cancers: Overview > Proteoglycans in cancer.

References

1). A human organoid drug screen identifies α2-adrenergic receptor signaling as a therapeutic target for cartilage regeneration. Cell stem cell, 2024 (PubMed: 39353427) [IF=19.8]

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