Product: PNPLA2 Antibody
Catalog: DF7756
Description: Rabbit polyclonal antibody to PNPLA2
Application: WB IHC IF/ICC
Cited expt.: WB
Reactivity: Human, Mouse
Mol.Wt.: 54 kDa; 55kD(Calculated).
Uniprot: Q96AD5
RRID: AB_2841222

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Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Clonality:
Polyclonal
Specificity:
PNPLA2 Antibody detects endogenous levels of total PNPLA2.
RRID:
AB_2841222
Cite Format: Affinity Biosciences Cat# DF7756, RRID:AB_2841222.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

1110001C14Rik; Adipose triglyceride lipase; ATGL; ATGL DESNUTRIN; Calcium independent phospholipase A2; Calcium-independent phospholipase A2; Desnutrin; EC 3.1.1.3; FP17548; IPLA2 zeta; IPLA2-zeta; Mutant patatin like phospholipase domain containing 2; Patatin like phospholipase domain containing 2; PATATIN LIKE PHOSPHOLIPASE DOMAIN CONTAINING PROTEIN 2; Patatin-like phospholipase domain-containing protein 2; PEDF R; PHOSPHOLIPASE A2 CALCIUM INDEPENDENT ZETA; Pigment epithelium derived factor; Pigment epithelium-derived factor; plpl; plpl2; PLPL2_HUMAN; Pnpla2; Transport secretion protein 2; Transport secretion protein 2.2; Transport-secretion protein 2; Triglyceride hydrolase; TTS 2.2; TTS2; TTS2.2; ZETA;

Immunogens

Immunogen:

A synthesized peptide derived from human PNPLA2, corresponding to a region within the internal amino acids.

Uniprot:
Gene(ID):
Expression:
Q96AD5 PLPL2_HUMAN:

Highest expression in adipose tissue. Also detected in heart, skeletal muscle, and portions of the gastrointestinal tract. Detected in normal retina and retinoblastoma cells. Detected in retinal pigment epithelium and, at lower intensity, in the inner segments of photoreceptors and in the ganglion cell layer of the neural retina (at protein level).

Sequence:
MFPREKTWNISFAGCGFLGVYYVGVASCLREHAPFLVANATHIYGASAGALTATALVTGVCLGEAGAKFIEVSKEARKRFLGPLHPSFNLVKIIRSFLLKVLPADSHEHASGRLGISLTRVSDGENVIISHFNSKDELIQANVCSGFIPVYCGLIPPSLQGVRYVDGGISDNLPLYELKNTITVSPFSGESDICPQDSSTNIHELRVTNTSIQFNLRNLYRLSKALFPPEPLVLREMCKQGYRDGLRFLQRNGLLNRPNPLLALPPARPHGPEDKDQAVESAQAEDYSQLPGEDHILEHLPARLNEALLEACVEPTDLLTTLSNMLPVRLATAMMVPYTLPLESALSFTIRLLEWLPDVPEDIRWMKEQTGSICQYLVMRAKRKLGRHLPSRLPEQVELRRVQSLPSVPLSCAAYREALPGWMRNNLSLGDALAKWEECQRQLLLGLFCTNVAFPPEALRMRAPADPAPAPADPASPQHQLAGPAPLLSTPAPEARPVIGALGL

Research Backgrounds

Function:

Catalyzes the initial step in triglyceride hydrolysis in adipocyte and non-adipocyte lipid droplets. Also has acylglycerol transacylase activity. May act coordinately with LIPE/HLS within the lipolytic cascade. Regulates adiposome size and may be involved in the degradation of adiposomes. May play an important role in energy homeostasis. May play a role in the response of the organism to starvation, enhancing hydrolysis of triglycerides and providing free fatty acids to other tissues to be oxidized in situations of energy depletion.

PTMs:

Phosphorylation at Ser-404 by PKA is increased during fasting and moderate intensity exercise, and moderately increases lipolytic activity (By similarity). Phosphorylation at Ser-404 is increased upon beta-adrenergic stimulation.

Subcellular Location:

Lipid droplet. Cell membrane>Single-pass type II membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Highest expression in adipose tissue. Also detected in heart, skeletal muscle, and portions of the gastrointestinal tract. Detected in normal retina and retinoblastoma cells. Detected in retinal pigment epithelium and, at lower intensity, in the inner segments of photoreceptors and in the ganglion cell layer of the neural retina (at protein level).

Research Fields

· Metabolism > Lipid metabolism > Glycerolipid metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

· Organismal Systems > Endocrine system > Regulation of lipolysis in adipocytes.

References

1). PLIN1 suppresses glioma progression through regulating lipid metabolism. Cell death & disease, 2025 (PubMed: 39870645) [IF=8.1]

2). Restoration of lipid homeostasis between TG and PE by the LXRα-ATGL/EPT1 axis ameliorates hepatosteatosis. Cell death & disease, 2023 (PubMed: 36746922) [IF=8.1]

Application: WB    Species: Mouse    Sample:

Fig. 4: Decrease of Atgl and Ept1 is an important cause of lipid disorders. A Investigating genetic mechanisms affecting lipid homeostasis through a working model of lipid homeostasis pathways including glycerolipid metabolism and synthesis process. G-3-P glycerol-3-phosphate, LPA lysophosphatidic acid, PA phosphatidic acid, DG diglyceride, TG triglyceride, FFA free fatty acid, Etn ethanolamine, P-Etn phosphoethanolamine, CDP-Etn CDP-ethanolamine, PE phosphatidylethanolamine, LPE lysophosphatidylethanolamine, PC phosphatidylcholine. B Heatmap of genes related to lipid catabolism and synthesis (data obtained from GEO database with an accession number of GSE204986). C Correlation heatmap between total lipid metabolites and genes associated with TG metabolism and PE synthesis (n = 5 per group). D Pearson’s correlation between Atgl expression and TG content, and Pearson’s correlation between Ept1 expression and PE content. E Correlation heatmap between species of TG containing different lengths of fatty acids and genes associated with TG metabolism and PE synthesis. F Pearson’s correlation between Atgl expression and TG(54:7) or PE(48:1) content. G Correlation heatmap between species of PE containing different lengths of fatty acids and genes associated with TG metabolism and PE synthesis. H Pearson’s correlation between Ept1 expression and PE(36:3) or PE(40:7) content. I Correlation heatmap between mice phenotypes and genes associated with TG metabolism and PE synthesis. J Western blot analysis of LXRα, ATGL and EPT1 proteins in mice from group WT Chow, LXRα−/− Chow, WT HFD and LXRα-/− HFD (n = 3 per group). Data are presented as mean ± SD. *P 

3). Yogurt-derived Lactobacillus plantarum Q16 alleviated high-fat diet-induced non-alcoholic fatty liver disease in mice. Food Science and Human Wellness, 2022 [IF=7.0]

Application: WB    Species: Mouse    Sample:

Fig. 5. Effects ofL. plantarum Q16 on key proteins involved in hepatic lipid metabolism in HFD-fed obese mice. Data are presented as mean ± SD (n = 6). Different lowercase alphabet letters were significantly different at the level of P < 0.05.

4). Oridonin restores hepatic lipid homeostasis in an LXRα-ATGL/EPT1 axis-dependent manner. Journal of pharmaceutical analysis, 2023 (PubMed: 38174118) [IF=6.1]

Application: WB    Species: Mouse    Sample:

Fig. 6. Oridonin (ORI) protects against hepatic steatosis by restoring lipid homeostasis between triglyceride (TG) and phosphatidylethanolamine (PE) via the LXRα-ATGL/EPT1 pathway. (A) Schematic showing how the ORI affects lipid homeostasis. (B) Representative T1-MRI images showing lipid accumulating contents in livers of WT and LXRα−/− mice induced a high-fat diet (HFD) with or without ORI treatment. (C) Representative in vivo imaging system images of mice after tail vein injection of cy7-labeled phosphoethanolamine. The insert shows representative images of cy7-labeled phosphoethanolamine fluorescence intensity in mice liver, kidney and intestine. Mice livers, kidneys and intestines were collected at 24 h after tail vein injection of cy7-labeled phosphoethanolamine. (D) Representative in vivo imaging system images of mice after tail vein injection of cy7-labeled cholesterol. The insert shows representative images of cy7-labeled cholesterol fluorescence intensity in mice liver. Mice livers were collected at 6 h after tail vein injection of cy7-labeled cholesterol. (E, F) Protein expression of LXRα, ATGL and EPT1 in liver tissues of WT mice after continuously treated with various doses of ORI for 7 days (n = 3). Data are presented as mean ± standard deviation (SD). ∗P < 0.05 and ∗∗P < 0.01 were compared with vehicle group. (G, H) Protein expression of LXRα, and EPT1 in the livers from WT and LXRα−/− mice in the indicated groups. #P < 0.05 and ##P < 0.01 were compared with Chow group; ∗P < 0.05 and ∗∗P < 0.01 were compared with HFD group. TG: triglyceride; DG: diglyceride; P-Etn: phosphoethanolamine; CDP-Etn: CDP-ethanolamine; PE: phosphatidylethanolamine.

5). Probiotic Yogurt Alleviates High-Fat Diet-Induced Lipid Accumulation and Insulin Resistance in Mice via the Adiponectin Pathway. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2023 (PubMed: 36695046) [IF=5.7]

6). Equisetin inhibits adiposity through AMPK-dependent regulation of brown adipocyte differentiation. Heliyon, 2024 (PubMed: 38327434) [IF=4.0]

7). Fufang Zhenzhu Tiaozhi Capsule Prevents Intestinal Inflammation and Barrier Disruption in Mice With Non-Alcoholic Steatohepatitis. Frontiers in Endocrinology, 2022 (PubMed: 35784533) [IF=3.9]

8). Indirect regulation of HIPPO pathway by miRNA mediates high-intensity intermittent exercise to ameliorate aging skeletal muscle function. Scandinavian journal of medicine & science in sports, 2023 (PubMed: 36789636) [IF=3.5]

9). Effects of high-intensity interval training on adipose tissue lipolysis, inflammation, and metabolomics in aged rats. PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY, 2020 (PubMed: 32006095) [IF=2.9]

Application: WB    Species: rat    Sample: fat

Fig. 4 |Metabolic pathway enrichment analysis and lipolysis metabolismrelated protein and mRNA expression. Correlation analysis of 9 metabolites (a); metabolic pathway analysis in MICT versus SED (b);mRNA levels of PPAR-γ, HSL, ATGL, and TNF-α in adipose tissues (c);correlation analysis of 6 metabolites (d); metabolic pathway analysis in HIIT versus SED (e); and contents of PPAR-γ, HSL, ATGL, TNF-α, and P450scc protein (f).

10). Fibroblast Growth Factor Receptor 4 Promotes Triple-Negative Breast Cancer Progression via Regulating Fatty Acid Metabolism Through the AKT/RYR2 Signaling. Cancer medicine, 2024 (PubMed: 39658878) [IF=2.9]

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