Product: Phospho-MSK1 (Ser212) Antibody
Catalog: AF3031
Description: Rabbit polyclonal antibody to Phospho-MSK1 (Ser212)
Application: WB IHC IF/ICC
Reactivity: Human, Mouse
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 90kDa; 90kD(Calculated).
Uniprot: O75582
RRID: AB_2834461

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 100ul $280 In stock
 200ul $350 In stock

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Product Info

IF/ICC 1:100-1:500, WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Pig(80%), Zebrafish(80%), Bovine(90%), Horse(90%), Sheep(90%), Rabbit(100%), Dog(90%), Chicken(90%), Xenopus(90%)
Phospho-MSK1 (Ser212) Antibody detects endogenous levels of MSK1 only when phosphorylated at Serine 212.
Cite Format: Affinity Biosciences Cat# AF3031, RRID:AB_2834461.
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.


90 kDa ribosomal protein S6 kinase 5; EC; KS6A5_HUMAN; MGC1911; Mitogen and stress activated protein kinase 1; MSPK1; Nuclear Mitogen And Stress Activated Protein Kinase 1; Nuclear mitogen- and stress-activated protein kinase 1; Ribosomal protein S6 kinase 90kD polypeptide 5; Ribosomal protein S6 kinase 90kDa; Ribosomal protein S6 kinase 90kDa polypeptide 5; Ribosomal Protein S6 Kinase Alpha 5; Ribosomal protein S6 kinase alpha-5; RLPK; RPS6KA5; RSK Like Protein Kinase; RSK-like protein kinase; RSKL; S6K alpha 5; S6K-alpha-5;


O75582 KS6A5_HUMAN:

Widely expressed with high levels in heart, brain and placenta. Less abundant in lung, kidney and liver.

an AGC kinase of the RSK family. Has two kinase domains connected by a regulatory linker region. Enzyme activity requires the presence of both kinase domains. Is phosphorylated and activated by Erk as well as p38 MAPK in response to growth factors and cellular stress.



Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O75582 As Substrate

Site PTM Type Enzyme
T38 Phosphorylation
K53 Ubiquitination
Y60 Phosphorylation
Y175 Phosphorylation
S199 Phosphorylation
S212 Phosphorylation O75582 (RPS6KA5)
S240 Phosphorylation
S253 Phosphorylation
S268 Phosphorylation
K273 Ubiquitination
K313 Ubiquitination
K329 Ubiquitination
S360 Phosphorylation P27361 (MAPK3) , Q16539 (MAPK14) , Q15759 (MAPK11) , P28482 (MAPK1)
Y375 Phosphorylation
S376 Phosphorylation Q16539 (MAPK14) , O75582 (RPS6KA5)
S381 Phosphorylation O75582 (RPS6KA5)
K385 Ubiquitination
S436 Phosphorylation
S448 Phosphorylation
K473 Ubiquitination
K527 Ubiquitination
K546 Ubiquitination
T581 Phosphorylation Q15759 (MAPK11) , P28482 (MAPK1) , Q16539 (MAPK14) , P27361 (MAPK3)
S628 Phosphorylation
T630 Phosphorylation
S647 Phosphorylation
K654 Ubiquitination
S657 Phosphorylation
K661 Ubiquitination
K674 Ubiquitination
K677 Ubiquitination
Y683 Phosphorylation
S691 Phosphorylation
S694 Phosphorylation
S695 Phosphorylation
T700 Phosphorylation P28482 (MAPK1) , Q16539 (MAPK14) , P27361 (MAPK3) , Q15759 (MAPK11)
S750 Phosphorylation O75582 (RPS6KA5)
T751 Phosphorylation
S752 Phosphorylation O75582 (RPS6KA5)
S757 Phosphorylation P68400 (CSNK2A1)
S758 Phosphorylation P68400 (CSNK2A1) , O75582 (RPS6KA5)
S759 Phosphorylation P68400 (CSNK2A1)
S760 Phosphorylation P68400 (CSNK2A1)
S766 Phosphorylation
S770 Phosphorylation
T793 Phosphorylation P68400 (CSNK2A1)

PTMs - O75582 As Enzyme

Substrate Site Source
O75582 (RPS6KA5) S212 Uniprot
O75582 (RPS6KA5) S376 Uniprot
O75582 (RPS6KA5) S381 Uniprot
O75582 (RPS6KA5) S750 Uniprot
O75582 (RPS6KA5) S752 Uniprot
O75582 (RPS6KA5) S758 Uniprot
P05114 (HMGN1) S7 Uniprot
P07101-1 (TH) S40 Uniprot
P0C0S8 (HIST1H2AM) S2 Uniprot
P10276 (RARA) S369 Uniprot
P10636-5 (MAPT) S214 Uniprot
P10636-5 (MAPT) S262 Uniprot
P11831 (SRF) S103 Uniprot
P16220 (CREB1) S133 Uniprot
P18846-1 (ATF1) S63 Uniprot
P19419 (ELK1) S383 Uniprot
P22736 (NR4A1) S351 Uniprot
P40763-2 (STAT3) S726 Uniprot
P40763 (STAT3) S727 Uniprot
P42224 (STAT1) S727 Uniprot
P47712 (PLA2G4A) S727 Uniprot
P50549-1 (ETV1) S191 Uniprot
P50549-1 (ETV1) S216 Uniprot
P50549-1 (ETV1) S334 Uniprot
P54253 (ATXN1) S775 Uniprot
P68431 (HIST1H3J) S11 Uniprot
P68431 (HIST1H3J) S29 Uniprot
P84243 (H3F3B) S11 Uniprot
P84243 (H3F3B) S29 Uniprot
Q04206 (RELA) S276 Uniprot
Q13541 (EIF4EBP1) T36 Uniprot
Q13541 (EIF4EBP1) T45 Uniprot
Q13541 (EIF4EBP1) S65 Uniprot
Q15831-1 (STK11) S428 Uniprot
Q16695 (HIST3H3) S11 Uniprot
Q16695 (HIST3H3) S29 Uniprot
Q7L7L0 (HIST3H2A) S2 Uniprot
Q92934 (BAD) S75 Uniprot
Q92934 (BAD) S153 Uniprot
Q9H4A3 (WNK1) T60 Uniprot

Research Backgrounds


Serine/threonine-protein kinase that is required for the mitogen or stress-induced phosphorylation of the transcription factors CREB1 and ATF1 and for the regulation of the transcription factors RELA, STAT3 and ETV1/ER81, and that contributes to gene activation by histone phosphorylation and functions in the regulation of inflammatory genes. Phosphorylates CREB1 and ATF1 in response to mitogenic or stress stimuli such as UV-C irradiation, epidermal growth factor (EGF) and anisomycin. Plays an essential role in the control of RELA transcriptional activity in response to TNF and upon glucocorticoid, associates in the cytoplasm with the glucocorticoid receptor NR3C1 and contributes to RELA inhibition and repression of inflammatory gene expression. In skeletal myoblasts is required for phosphorylation of RELA at 'Ser-276' during oxidative stress. In erythropoietin-stimulated cells, is necessary for the 'Ser-727' phosphorylation of STAT3 and regulation of its transcriptional potential. Phosphorylates ETV1/ER81 at 'Ser-191' and 'Ser-216', and thereby regulates its ability to stimulate transcription, which may be important during development and breast tumor formation. Directly represses transcription via phosphorylation of 'Ser-1' of histone H2A. Phosphorylates 'Ser-10' of histone H3 in response to mitogenics, stress stimuli and EGF, which results in the transcriptional activation of several immediate early genes, including proto-oncogenes c-fos/FOS and c-jun/JUN. May also phosphorylate 'Ser-28' of histone H3. Mediates the mitogen- and stress-induced phosphorylation of high mobility group protein 1 (HMGN1/HMG14). In lipopolysaccharide-stimulated primary macrophages, acts downstream of the Toll-like receptor TLR4 to limit the production of pro-inflammatory cytokines (By similarity). Functions probably by inducing transcription of the MAP kinase phosphatase DUSP1 and the anti-inflammatory cytokine interleukin 10 (IL10), via CREB1 and ATF1 transcription factors (By similarity). Plays a role in neuronal cell death by mediating the downstream effects of excitotoxic injury (By similarity). Phosphorylates TRIM7 at 'Ser-107' in response to growth factor signaling via the MEK/ERK pathway, thereby stimulating its ubiquitin ligase activity.


Ser-376 and Thr-581 phosphorylation is required for kinase activity. Ser-376 and Ser-212 are autophosphorylated by the C-terminal kinase domain, and their phosphorylation is essential for the catalytic activity of the N-terminal kinase domain. Phosphorylated at Ser-360, Thr-581 and Thr-700 by MAPK1/ERK2, MAPK3/ERK1 and MAPK14/p38-alpha. Autophosphorylated at Ser-750, Ser-752 and Ser-758 by the N-terminal kinase domain.


Subcellular Location:

Nucleus. Cytoplasm.
Note: Predominantly nuclear. Exported into cytoplasm in response to glucocorticoid.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Widely expressed with high levels in heart, brain and placenta. Less abundant in lung, kidney and liver.

Subunit Structure:

Forms a complex with either MAPK1/ERK2 or MAPK3/ERK1 in quiescent cells which transiently dissociates following mitogenic stimulation. Also associates with MAPK14/p38-alpha. Activated RPS6KA5 associates with and phosphorylates the NF-kappa-B p65 subunit RELA. Interacts with CREBBP and EP300.


Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily.

Research Fields

· Environmental Information Processing > Signal transduction > MAPK signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > TNF signaling pathway.   (View pathway)

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Human Diseases > Cancers: Overview > MicroRNAs in cancer.

· Human Diseases > Cancers: Specific types > Bladder cancer.   (View pathway)

· Organismal Systems > Circulatory system > Adrenergic signaling in cardiomyocytes.   (View pathway)

· Organismal Systems > Environmental adaptation > Circadian entrainment.

· Organismal Systems > Nervous system > Neurotrophin signaling pathway.   (View pathway)

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