PDCD6IP Antibody - #DF9027

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Product: PDCD6IP Antibody
Catalog: DF9027
Description: Rabbit polyclonal antibody to PDCD6IP
Application: WB
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 96 kDa; 96kD(Calculated).
Uniprot: Q8WUM4
RRID: AB_2842223

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(91%)
Clonality:
Polyclonal
Specificity:
PDCD6IP Antibody detects endogenous levels of total PDCD6IP.
RRID:
AB_2842223
Cite Format: Affinity Biosciences Cat# DF9027, RRID:AB_2842223.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

AIP1; ALG 2 interacting protein 1; ALG-2-interacting protein 1; ALG2 interacting protein X; Alix; Apoptosis linked gene 2 interacting protein X; Dopamine receptor interacting protein 4; DRIP4; Hp95; KIAA1375; MGC17003; PDC6I_HUMAN; PDCD6 interacting protein; PDCD6-interacting protein; PDCD6IP; Programmed cell death 6 interacting protein; Programmed cell death 6-interacting protein;

Immunogens

Immunogen:
Uniprot:

Q8WUM4(PDC6I_HUMAN) >>Visit HPA database.

Gene(ID):
PDCD6IP(10015)
Sequence:
MATFISVQLKKTSEVDLAKPLVKFIQQTYPSGGEEQAQYCRAAEELSKLRRAAVGRPLDKHEGALETLLRYYDQICSIEPKFPFSENQICLTFTWKDAFDKGSLFGGSVKLALASLGYEKSCVLFNCAALASQIAAEQNLDNDEGLKIAAKHYQFASGAFLHIKETVLSALSREPTVDISPDTVGTLSLIMLAQAQEVFFLKATRDKMKDAIIAKLANQAADYFGDAFKQCQYKDTLPKEVFPVLAAKHCIMQANAEYHQSILAKQQKKFGEEIARLQHAAELIKTVASRYDEYVNVKDFSDKINRALAAAKKDNDFIYHDRVPDLKDLDPIGKATLVKSTPVNVPISQKFTDLFEKMVPVSVQQSLAAYNQRKADLVNRSIAQMREATTLANGVLASLNLPAAIEDVSGDTVPQSILTKSRSVIEQGGIQTVDQLIKELPELLQRNREILDESLRLLDEEEATDNDLRAKFKERWQRTPSNELYKPLRAEGTNFRTVLDKAVQADGQVKECYQSHRDTIVLLCKPEPELNAAIPSANPAKTMQGSEVVNVLKSLLSNLDEVKKEREGLENDLKSVNFDMTSKFLTALAQDGVINEEALSVTELDRVYGGLTTKVQESLKKQEGLLKNIQVSHQEFSKMKQSNNEANLREEVLKNLATAYDNFVELVANLKEGTKFYNELTEILVRFQNKCSDIVFARKTERDELLKDLQQSIAREPSAPSIPTPAYQSSPAGGHAPTPPTPAPRTMPPTKPQPPARPPPPVLPANRAPSATAPSPVGAGTAAPAPSQTPGSAPPPQAQGPPYPTYPGYPGYCQMPMPMGYNPYAYGQYNMPYPPVYHQSPGQAPYPGPQQPSYPFPQPPQQSYYPQQ

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Dog
100
Zebrafish
100
Chicken
100
Rabbit
100
Xenopus
91
Sheep
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q8WUM4 As Substrate

Site PTM Type Enzyme
A2 Acetylation
K10 Methylation
S13 Phosphorylation
K19 Ubiquitination
K23 Acetylation
K23 Ubiquitination
Y39 Phosphorylation
C40 S-Nitrosylation
K48 Acetylation
K48 Ubiquitination
K60 Acetylation
K60 Ubiquitination
K101 Ubiquitination
S103 Phosphorylation
S108 Phosphorylation
T166 Phosphorylation
S169 Phosphorylation
T186 Phosphorylation
K209 Ubiquitination
K215 Acetylation
K215 Ubiquitination
Y223 Phosphorylation
K229 Ubiquitination
K234 Ubiquitination
K239 Ubiquitination
C250 S-Nitrosylation
Y258 Phosphorylation
K269 Ubiquitination
K285 Ubiquitination
Y294 Phosphorylation
K298 Ubiquitination
K303 Ubiquitination
K313 Ubiquitination
Y319 Phosphorylation
R322 Methylation
K327 Ubiquitination
K334 Ubiquitination
K339 Ubiquitination
K350 Acetylation
K350 Ubiquitination
K357 Ubiquitination
S366 Phosphorylation
Y370 Phosphorylation
K438 Ubiquitination
R456 Methylation
T464 Phosphorylation
K471 Ubiquitination
T479 Phosphorylation
S481 Phosphorylation
Y485 Phosphorylation
K486 Acetylation
K486 Ubiquitination
K501 Ubiquitination
K510 Ubiquitination
K541 Ubiquitination
K563 Ubiquitination
S575 Phosphorylation
R606 Methylation
Y608 Phosphorylation
T612 Phosphorylation
T613 Phosphorylation
K614 Ubiquitination
K627 Ubiquitination
K638 Ubiquitination
K640 Ubiquitination
S692 Phosphorylation
K707 Ubiquitination
S712 Phosphorylation
S718 Phosphorylation
S721 Phosphorylation
T724 Phosphorylation
Y727 Phosphorylation
S729 Phosphorylation
S730 Phosphorylation
T738 Phosphorylation
T741 Phosphorylation
R745 Methylation
K751 Acetylation
K751 Methylation
K751 Ubiquitination
R757 Methylation
R767 Methylation

Research Backgrounds

Function:

Multifunctional protein involved in endocytosis, multivesicular body biogenesis, membrane repair, cytokinesis, apoptosis and maintenance of tight junction integrity. Class E VPS protein involved in concentration and sorting of cargo proteins of the multivesicular body (MVB) for incorporation into intralumenal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome. Binds to the phospholipid lysobisphosphatidic acid (LBPA) which is abundant in MVBs internal membranes. The MVB pathway requires the sequential function of ESCRT-O, -I,-II and -III complexes. The ESCRT machinery also functions in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis. Adapter for a subset of ESCRT-III proteins, such as CHMP4, to function at distinct membranes. Required for completion of cytokinesis. May play a role in the regulation of both apoptosis and cell proliferation. Regulates exosome biogenesis in concert with SDC1/4 and SDCBP. By interacting with F-actin, PARD3 and TJP1 secures the proper assembly and positioning of actomyosin-tight junction complex at the apical sides of adjacent epithelial cells that defines a spatial membrane domain essential for the maintenance of epithelial cell polarity and barrier (By similarity).

(Microbial infection) Involved in HIV-1 virus budding. Can replace TSG101 it its role of supporting HIV-1 release; this function requires the interaction with CHMP4B. The ESCRT machinery also functions in topologically equivalent membrane fission events, such as enveloped virus budding (HIV-1 and other lentiviruses).

PTMs:

May be phosphorylated on tyrosine residues by activated PDGFRB.

Subcellular Location:

Cytoplasm>Cytosol. Melanosome. Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome. Secreted>Extracellular exosome. Cell junction>Tight junction. Midbody>Midbody ring.
Note: Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Colocalized with CEP55 at centrosomes of non-dividing cells. Component of the actomyosin-tight junction complex (By similarity). PDCD6IP targeting to the midbody requires the interaction with CEP55 (PubMed:18641129).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Self-associates. Interacts with SH3KBP1/CIN85 (By similarity). Interacts with PDCD6 in a calcium -dependent manner. Interacts with TSG101 in a calcium-dependent manner; PDCD6IP homooligomerization may be required for TSG101-binding. Interacts with SGSM3. Directly interacts with CHMP4A, CHMP4B and CHMP4C. Directly interacts with CEP55 in a 1:2 stoechiometry. The interaction with CEP55 is required for PDCD6IP targeting to the midbody. May interact with PDGFRB. Interacts with SH3GL1 and SH3GL2/endophilin-1. Forms a complex with SDCBP and SDC2. Found in a complex with F-actin, TJP1/ZO-1 and PARD3 (By similarity). Interacts with CD2AP. Interacts with ARRDC1.

(Microbial infection) Interacts with HIV-1 p6. Interacts with HIV-1 p9.

(Microbial infection) Interacts with EIAV p9.

(Microbial infection) Interacts with Murine leukemia virus Gag polyprotein (via LYPX(n)L motif).

(Microbial infection) Interacts with ebola virus protein VP40 (via YPx(n)L/I motif).

Research Fields

· Cellular Processes > Transport and catabolism > Endocytosis.   (View pathway)

References

1). Endometrial extracellular vesicles from women with recurrent implantation failure attenuate the growth and invasion of embryos. FERTILITY AND STERILITY, 2020 (PubMed: 32622655) [IF=6.6]

Application: WB    Species: Human    Sample: RIF-EVs and FER-EVs.

Figure 1.Isolation of EVs and determination of RIF-EVs and FER-EVs. (A) Western blotting shows that RIF-EVs and FER-EVs expressed classic EV protein markers Alix, TSG101, and CD9. Representative shapes of (B) RIF-EVs and (C) FER-EVs detected with the use of transmission electron microscopy. The size and distribution of (D) RIF-EVs and (E) FER-EVs examined with the use of nanoparticle tracking analysis. ECs ¼ endometrial cells; EVs ¼ extracellular vesicles; FER ¼ fertile women; RIF ¼ women with recurrent implantation failure. Liu. Extracellular vesicles regulate embryos. Fertil Steril 2020.
2). Discovery and validation of extracellular vesicle‐associated miRNAs as noninvasive detection biomarkers for early‐stage non‐small‐cell lung cancer. Molecular Oncology, 2021 (PubMed: 33340250) [IF=5.0]

Application: WB    Species: Human    Sample: lung tissues

Figure 1. Characterisation of EVs derived from the serum and plasma of NSCLC patients and controls. (a) The shape and structure of serum and plasma EVs isolated by EXOquick kit under TEM. The red arrow represents EVs with typical characteristics (scale bars are 200 nm). (b) The size of EVs derived from control groups and NSCLC groups was analysed by NTA. (c) Western blots of EVs membrane markers, including Alix, CD63, TSG101, CD9, and one negative marker ALB.
3). Human Umbilical Cord Mesenchymal Stem Cells Improve Ovarian Function in Chemotherapy-Induced Premature Ovarian Failure Mice Through Inhibiting Apoptosis and Inflammation via a Paracrine Mechanism. Reproductive Sciences, 2021 (PubMed: 33751459) [IF=2.6]

Application: WB    Species: Human    Sample: granulosa cells

Fig. 6 Identification of UC-MSC-EVs and effects of UC-MSC-EVs on NM-treated granulosa cells in vitro. a Nanoparticle tracking analysis, b the image under transmission electron microscope, and c the protein expression of EV markers for EVs extracted from UC-MSCs culture medium. After NM-KGN cells treated with or without UC-MSC-EVs for 48 h, d the morphology of KGN cells was observed, and e the survival rates were examined by CCK-8 assays. f The IL-6 and IL-1β concentrations of NM-KGN cells (adjusted by the protein concentrations) treated with or without UC-MSC-EVs. g Flow cytometric analysis of AnnexinV/ PI staining levels of KGN cells. h Comparisons of total, early and late apoptosis rates of KGN cells among the three groups. *P < 0.05. **P < 0.01
4). Pancreatic Cancer-Derived Exosomes Promote the Proliferation, Invasion, and Metastasis of Pancreatic Cancer by the miR-3960/TFAP2A Axis. Journal of Oncology, 2022 (PubMed: 36284637)

Application: WB    Species: Mice    Sample:

Figure 2 The identification of PANC-1-derived exosomes. (x¯¯±s, n =3). (a) Transmission electron microscopy. (Scale bar =200 nm) (b and c) Nanoparticle Tracking analysis. (d) The average levels of TSG101, Alix, CD81, CD9, CD63, HSP70 and C-myc. ∗∗P <0.01 compared to the cell group.

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