Product: HSD17B12 Antibody
Catalog: DF9505
Description: Rabbit polyclonal antibody to HSD17B12
Application: WB IHC IF/ICC
Reactivity: Human
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.: 34 kDa; 34kD(Calculated).
Uniprot: Q53GQ0
RRID: AB_2842701

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 100ul $280 In stock
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Product Info

Source:
Rabbit
Application:
IF/ICC 1:100-1:500, IHC 1:50-1:200, WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Prediction:
Pig(100%), Bovine(100%), Horse(88%), Sheep(100%), Rabbit(88%), Dog(100%), Chicken(88%)
Clonality:
Polyclonal
Specificity:
HSD17B12 Antibody detects endogenous levels of total HSD17B12.
RRID:
AB_2842701
Cite Format: Affinity Biosciences Cat# DF9505, RRID:AB_2842701.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

12,3-ketoacyl-CoA reductase,short chain; 17 beta HSD 12; 17 beta hydroxysteroid dehydrogenase 12; 17-@beta-HSD XII; 17-@beta-hydroxysteroid dehydrogenase XII; 17-beta-HSD 12; 17-beta-hydroxysteroid dehydrogenase 12; 17beta HSD type 12; 3 ketoacyl CoA reductase; 3-ketoacyl-CoA reductase; DHB12_HUMAN; Estradiol 17 beta dehydrogenase 12; Estradiol 17-beta-dehydrogenase 12; HSD17B12; Hydroxysteroid (17-beta) dehydrogenase 12; KAR; KIK-I; Kik1; SDR12C1; Short chain dehydrogenase/reductase family 12C member 1; Steroid dehydrogenase homolog;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
Q53GQ0 DHB12_HUMAN:

Expressed in most tissues tested. Highly expressed in the ovary and mammary. Expressed in platelets.

Sequence:
MESALPAAGFLYWVGAGTVAYLALRISYSLFTALRVWGVGNEAGVGPGLGEWAVVTGSTDGIGKSYAEELAKHGMKVVLISRSKDKLDQVSSEIKEKFKVETRTIAVDFASEDIYDKIKTGLAGLEIGILVNNVGMSYEYPEYFLDVPDLDNVIKKMININILSVCKMTQLVLPGMVERSKGAILNISSGSGMLPVPLLTIYSATKTFVDFFSQCLHEEYRSKGVFVQSVLPYFVATKLAKIRKPTLDKPSPETFVKSAIKTVGLQSRTNGYLIHALMGSIISNLPSWIYLKIVMNMNKSTRAHYLKKTKKN

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Bovine
100
Sheep
100
Dog
100
Horse
88
Chicken
88
Rabbit
88
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q53GQ0 As Substrate

Site PTM Type Enzyme
Y12 Phosphorylation
Y66 Phosphorylation
K72 Acetylation
K84 Ubiquitination
K86 Ubiquitination
S91 Phosphorylation
S92 Phosphorylation
K95 Ubiquitination
K97 Ubiquitination
S111 Phosphorylation
Y115 Phosphorylation
K117 Acetylation
K117 Ubiquitination
K119 Ubiquitination
S164 Phosphorylation
T169 Phosphorylation
K181 Ubiquitination
C215 S-Nitrosylation
K223 Ubiquitination
Y233 Phosphorylation
K238 Ubiquitination
K241 Ubiquitination
K244 Ubiquitination
S251 Phosphorylation
K257 Ubiquitination
K261 Ubiquitination
K299 Ubiquitination

Research Backgrounds

Function:

Catalyzes the second of the four reactions of the long-chain fatty acids elongation cycle. This endoplasmic reticulum-bound enzymatic process, allows the addition of two carbons to the chain of long- and very long-chain fatty acids/VLCFAs per cycle. This enzyme has a 3-ketoacyl-CoA reductase activity, reducing 3-ketoacyl-CoA to 3-hydroxyacyl-CoA, within each cycle of fatty acid elongation. Thereby, it may participate in the production of VLCFAs of different chain lengths that are involved in multiple biological processes as precursors of membrane lipids and lipid mediators. May also catalyze the transformation of estrone (E1) into estradiol (E2) and play a role in estrogen formation.

Subcellular Location:

Endoplasmic reticulum membrane>Multi-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in most tissues tested. Highly expressed in the ovary and mammary. Expressed in platelets.

Subunit Structure:

Interacts with ELOVL1 and LASS2.

Family&Domains:

The di-lysine motif confers endoplasmic reticulum localization for type I membrane proteins.

Belongs to the short-chain dehydrogenases/reductases (SDR) family. 17-beta-HSD 3 subfamily.

Research Fields

· Metabolism > Lipid metabolism > Fatty acid elongation.

· Metabolism > Lipid metabolism > Steroid hormone biosynthesis.

· Metabolism > Lipid metabolism > Biosynthesis of unsaturated fatty acids.

· Metabolism > Global and overview maps > Metabolic pathways.

· Metabolism > Global and overview maps > Fatty acid metabolism.

Restrictive clause

 

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