TRAK2 Antibody | Affinity Biosciences

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IHC
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TRAK2 Antibody - Western blot analysis of TRAK2 using LOVO whole cell lysates.

TRAK2 Antibody - DF9963 at 1/100 staining rat heart tissue by IHC-P.

TRAK2 Antibody - DF9963 at 1/100 staining rat muscle tissue by IHC-P.

TRAK2 Antibody - DF9963 at 1/100 staining mouse heart tissue by IHC-P.

TRAK2 Antibody - DF9963 at 1/100 staining rat heart tissue by IHC-P.

TRAK2 Antibody - DF9963 staining A549 cells by IF/ICC.

Product:	TRAK2 Antibody
Catalog:	DF9963
Description:	Rabbit polyclonal antibody to TRAK2
Application:	WB IHC IF/ICC
Reactivity:	Human
Prediction:	Pig, Horse, Dog
Mol.Wt.:	101 kDa; 101kD(Calculated).
Uniprot:	O60296
RRID:	AB_2843157

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

IF/ICC 1:100-1:500, IHC 1:50-1:200, WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human

Prediction:

Pig(88%), Horse(88%), Dog(88%)

Clonality:

Polyclonal

Specificity:

TRAK2 Antibody detects endogenous levels of total TRAK2.

RRID:

AB_2843157
Cite Format: Affinity Biosciences Cat# DF9963, RRID:AB_2843157.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

ALS2CR 3; Amyotrophic lateral sclerosis 2 (juvenile) chromosome region candidate 3; Amyotrophic lateral sclerosis 2 chromosomal region candidate gene 3 protein; CALS C; GRIF 1; GRIF1; KIAA0549; OIP98; Trafficking kinesin-binding protein 2; Trafficking protein kinesin binding 2; Trak2; TRAK2_HUMAN;

Immunogens

Immunogen:

A synthesized peptide derived from human TRAK2, corresponding to a region within C-terminal amino acids.

Uniprot:

O60296(TRAK2_HUMAN) >>Visit HPA database.

Gene(ID):

TRAK2(66008)

Expression:

O60296 TRAK2_HUMAN:

Widely expressed, with highest expression in heart.

Sequence:

O60296 TRAK2_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MSQSQNAIFTSPTGEENLMNSNHRDSESITDVCSNEDLPEVELVSLLEEQLPQYRLKVDTLFLYENQDWTQSPHQRQHASDALSPVLAEETFRYMILGTDRVEQMTKTYNDIDMVTHLLAERDRDLELAARIGQALLKRNHVLSEQNESLEEQLGQAFDQVNQLQHELCKKDELLRIVSIASEESETDSSCSTPLRFNESFSLSQGLLQLEMLQEKLKELEEENMALRSKACHIKTETVTYEEKEQQLVSDCVKELRETNAQMSRMTEELSGKSDELIRYQEELSSLLSQIVDLQHKLKEHVIEKEELKLHLQASKDAQRQLTMELHELQDRNMECLGMLHESQEEIKELRSRSGPTAHLYFSQSYGAFTGESLAAEIEGTMRKKLSLDEESSLFKQKAQQKRVFDTVRIANDTRGRSISFPALLPIPGSNRSSVIMTAKPFESGLQQTEDKSLLNQGSSSEEVAGSSQKMGQPGPSGDSDLATALHRLSLRRQNYLSEKQFFAEEWQRKIQVLADQKEGVSGCVTPTESLASLCTTQSEITDLSSASCLRGFMPEKLQIVKPLEGSQTLYHWQQLAQPNLGTILDPRPGVITKGFTQLPGDAIYHISDLEEDEEEGITFQVQQPLEVEEKLSTSKPVTGIFLPPITSAGGPVTVATANPGKCLSCTNSTFTFTTCRILHPSDITQVTPSSGFPSLSCGSSGSSSSNTAVNSPALSYRLSIGESITNRRDSTTTFSSTMSLAKLLQERGISAKVYHSPISENPLQPLPKSLAIPSTPPNSPSHSPCPSPLPFEPRVHLSENFLASRPAETFLQEMYGLRPSRNPPDVGQLKMNLVDRLKRLGIARVVKNPGAQENGRCQEAEIGPQKPDSAVYLNSGSSLLGGLRRNQSLPVIMGSFAAPVCTSSPKMGVLKED

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

Horse

Dog

Bovine

Sheep

Zebrafish

Rabbit

Xenopus

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

Research Backgrounds

O60296_TRAK2_HUMAN

Function:

May regulate endosome-to-lysosome trafficking of membrane cargo, including EGFR.

PTMs:

O-glycosylated.

Subcellular Location:

Cytoplasm. Early endosome. Mitochondrion.
Note: Colocalizes with MGARP at the mitochondria. Translocates from the cytoplasm to the mitochondria in a MGARP-dependent manner (By similarity).

Tissue Specificity:

Widely expressed, with highest expression in heart.

Family&Domains:

Belongs to the milton family.

Research Fields

· Metabolism > Global and overview maps > Metabolic pathways.

· Organismal Systems > Nervous system > GABAergic synapse.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.

TRAK2 Antibody - #DF9963