Nav1.7 Antibody - #AF9120

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Product: Nav1.7 Antibody
Catalog: AF9120
Description: Rabbit polyclonal antibody to Nav1.7
Application: WB
Cited expt.: WB
Reactivity: Human, Mouse
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.: 220kDa; 226kD(Calculated).
Uniprot: Q15858
RRID: AB_2843310

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 100ul $280 In stock
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MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(90%), Dog(100%), Chicken(100%)
Clonality:
Polyclonal
Specificity:
Nav1.7 Antibody detects endogenous levels of total Nav1.7.
RRID:
AB_2843310
Cite Format: Affinity Biosciences Cat# AF9120, RRID:AB_2843310.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

ETHA; GEFSP7; hNE Na; hNE-Na; hNENa; NE NA; NENA; Neuroendocrine sodium channel; Peripheral sodium channel 1; PN1; Scn9a; SCN9A_HUMAN; Sodium channel protein type 9 subunit alpha; Sodium channel protein type IX subunit alpha; Sodium channel voltage gated type IX alpha; Sodium channel voltage gated type IX alpha polypeptide; Sodium channel voltage gated type IX alpha subunit; Voltage gated sodium channel alpha subunit Nav1.7; Voltage gated sodium channel subunit alpha Nav1; Voltage-gated sodium channel subunit alpha Nav1.7;

Immunogens

Immunogen:

A synthesized peptide derived from human Nav1.7, corresponding to a region within the internal amino acids.

Uniprot:

Q15858(SCN9A_HUMAN) >>Visit HPA database.

Gene(ID):
SCN9A(6335)
Expression:
Q15858 SCN9A_HUMAN:

Expressed strongly in dorsal root ganglion, with only minor levels elsewhere in the body, smooth muscle cells, MTC cell line and C-cell carcinoma. Isoform 1 is expressed preferentially in the central and peripheral nervous system. Isoform 2 is expressed preferentially in the dorsal root ganglion.

Sequence:
MAMLPPPGPQSFVHFTKQSLALIEQRIAERKSKEPKEEKKDDDEEAPKPSSDLEAGKQLPFIYGDIPPGMVSEPLEDLDPYYADKKTFIVLNKGKTIFRFNATPALYMLSPFSPLRRISIKILVHSLFSMLIMCTILTNCIFMTMNNPPDWTKNVEYTFTGIYTFESLVKILARGFCVGEFTFLRDPWNWLDFVVIVFAYLTEFVNLGNVSALRTFRVLRALKTISVIPGLKTIVGALIQSVKKLSDVMILTVFCLSVFALIGLQLFMGNLKHKCFRNSLENNETLESIMNTLESEEDFRKYFYYLEGSKDALLCGFSTDSGQCPEGYTCVKIGRNPDYGYTSFDTFSWAFLALFRLMTQDYWENLYQQTLRAAGKTYMIFFVVVIFLGSFYLINLILAVVAMAYEEQNQANIEEAKQKELEFQQMLDRLKKEQEEAEAIAAAAAEYTSIRRSRIMGLSESSSETSKLSSKSAKERRNRRKKKNQKKLSSGEEKGDAEKLSKSESEDSIRRKSFHLGVEGHRRAHEKRLSTPNQSPLSIRGSLFSARRSSRTSLFSFKGRGRDIGSETEFADDEHSIFGDNESRRGSLFVPHRPQERRSSNISQASRSPPMLPVNGKMHSAVDCNGVVSLVDGRSALMLPNGQLLPEVIIDKATSDDSGTTNQIHKKRRCSSYLLSEDMLNDPNLRQRAMSRASILTNTVEELEESRQKCPPWWYRFAHKFLIWNCSPYWIKFKKCIYFIVMDPFVDLAITICIVLNTLFMAMEHHPMTEEFKNVLAIGNLVFTGIFAAEMVLKLIAMDPYEYFQVGWNIFDSLIVTLSLVELFLADVEGLSVLRSFRLLRVFKLAKSWPTLNMLIKIIGNSVGALGNLTLVLAIIVFIFAVVGMQLFGKSYKECVCKINDDCTLPRWHMNDFFHSFLIVFRVLCGEWIETMWDCMEVAGQAMCLIVYMMVMVIGNLVVLNLFLALLLSSFSSDNLTAIEEDPDANNLQIAVTRIKKGINYVKQTLREFILKAFSKKPKISREIRQAEDLNTKKENYISNHTLAEMSKGHNFLKEKDKISGFGSSVDKHLMEDSDGQSFIHNPSLTVTVPIAPGESDLENMNAEELSSDSDSEYSKVRLNRSSSSECSTVDNPLPGEGEEAEAEPMNSDEPEACFTDGCVWRFSCCQVNIESGKGKIWWNIRKTCYKIVEHSWFESFIVLMILLSSGALAFEDIYIERKKTIKIILEYADKIFTYIFILEMLLKWIAYGYKTYFTNAWCWLDFLIVDVSLVTLVANTLGYSDLGPIKSLRTLRALRPLRALSRFEGMRVVVNALIGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGKFYECINTTDGSRFPASQVPNRSECFALMNVSQNVRWKNLKVNFDNVGLGYLSLLQVATFKGWTIIMYAAVDSVNVDKQPKYEYSLYMYIYFVVFIIFGSFFTLNLFIGVIIDNFNQQKKKLGGQDIFMTEEQKKYYNAMKKLGSKKPQKPIPRPGNKIQGCIFDLVTNQAFDISIMVLICLNMVTMMVEKEGQSQHMTEVLYWINVVFIILFTGECVLKLISLRHYYFTVGWNIFDFVVVIISIVGMFLADLIETYFVSPTLFRVIRLARIGRILRLVKGAKGIRTLLFALMMSLPALFNIGLLLFLVMFIYAIFGMSNFAYVKKEDGINDMFNFETFGNSMICLFQITTSAGWDGLLAPILNSKPPDCDPKKVHPGSSVEGDCGNPSVGIFYFVSYIIISFLVVVNMYIAVILENFSVATEESTEPLSEDDFEMFYEVWEKFDPDATQFIEFSKLSDFAAALDPPLLIAKPNKVQLIAMDLPMVSGDRIHCLDILFAFTKRVLGESGEMDSLRSQMEERFMSANPSKVSYEPITTTLKRKQEDVSATVIQRAYRRYRLRQNVKNISSIYIKDGDRDDDLLNKKDMAFDNVNENSSPEKTDATSSTTSPPSYDSVTKPDKEKYEQDRTEKEDKGKDSKESKK

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Chicken
100
Rabbit
90
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

Research Backgrounds

Function:

Mediates the voltage-dependent sodium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a sodium-selective channel through which Na(+) ions may pass in accordance with their electrochemical gradient. It is a tetrodotoxin-sensitive Na(+) channel isoform. Plays a role in pain mechanisms, especially in the development of inflammatory pain.

PTMs:

Phosphorylation at Ser-1490 by PKC in a highly conserved cytoplasmic loop increases peak sodium currents.

Ubiquitinated by NEDD4L; which may promote its endocytosis. Does not seem to be ubiquitinated by NEDD4.

Subcellular Location:

Cell membrane>Multi-pass membrane protein. Cell projection>Neuron projection.
Note: In neurite terminals.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed strongly in dorsal root ganglion, with only minor levels elsewhere in the body, smooth muscle cells, MTC cell line and C-cell carcinoma. Isoform 1 is expressed preferentially in the central and peripheral nervous system. Isoform 2 is expressed preferentially in the dorsal root ganglion.

Family&Domains:

The sequence contains 4 internal repeats, each with 5 hydrophobic segments (S1, S2, S3, S5, S6) and one positively charged segment (S4). Segments S4 are probably the voltage-sensors and are characterized by a series of positively charged amino acids at every third position.

Belongs to the sodium channel (TC 1.A.1.10) family. Nav1.7/SCN9A subfamily.

Research Fields

· Organismal Systems > Sensory system > Taste transduction.

References

1). LncRNA01103/FGF13 axis promotes the progression of bone cancer pain by remodeling ac4C modification. Research Square, 2024

Application: WB    Species: Rat    Sample:

Figure 7. FGF13 participates in BCP by regulating sodium channels. (A) qPCR for monitoring FGF13-siRNA (siRNAFgf13) in RT4-D6P2T cells to validate transfection efficiency. Bars represent the means ± SEM of independent biological replicates (n = 3). Values of individual experiments are indicated as dots. P-values were calculated using the one-way ANOVA (**P < 0.01; ***P < 0.001). (B) Western blots for monitoring transfection efficiency of siRNAFgf13 in BCP rat SDH were validated. Bars represent the means ± SEM of independent biological replicates (n = 3). Values of individual experiments are indicated as dots. P-values were calculated using the one-way ANOVA (***P < 0.001). (C) Reducing Fgf13 expression attenuates mechanical nociceptive sensitization in BCP rats. Bars represent the means ± SEM of independent biological replicates (n = 8). P-values were calculated using the two-way repeated measures analysis (###P < 0.001). PWT: Paw withdrawal threshold. (D) Statistical plots of the number of buried strains in Sham, BCP+siRNANC and BCP+ siRNAFgf13 groups. Bars represent the means ± SEM of independent biological replicates (n = 8). Values of individual experiments are indicated as dots. P-values were calculated using the one-way ANOVA (**P < 0.01; ***P < 0.001). (E-G) Representative CatWalk gait, including footprint area and mean intensity (E), in Sham, BCP+ siRNANC and BCP+ siRNAFgf13 groups. Quantifications of mean intensity (LH/RH) (F) and print area (LH/RH) (G) in both groups of rats. Bars represent the means ± SEM of independent biological replicates (n = 8). Values of individual experiments are indicated as dots. P-values were calculated using the Student's t test (**P < 0.01; ***P < 0.001). LH, left hind paw; RH, right hind paw. (H-J) Representative traces of locomotor activity in the light-dark transition (LDT) (H) in Sham group, BCP+siRNANC group and BCP+siRNAFgf13 group rats. Quantifications of time spent (I) and distance (J) in the light chamber. Bars represent the means ± SEM of independent biological replicates (n = 8). Values of individual experiments are indicated as dots. P-values were calculated using the one-way ANOVA (**P < 0.01; ***P < 0.001). (K-L) Representative traces of Elevated plus- maze (EPM) (K) in Sham group, BCP+siRNANC group and BCP+siRNAFgf13 group rats. Quantifications of time spent (L) in the open arms. Bars represent the means ± SEM of independent biological replicates (n = 8). Values of individual experiments are indicated as dots. P-values were calculated using the one-way ANOVA (***P < 0.001). (M-P) Western blots for monitoring the effect of siRNAFgf13 on the expression of Nav1.1, Nav1.6, Nav1.7, Nav1.8 in the SDH of BCP rats. ß-Actin served as loading control. Bars represent the means ± SEM of independent biological replicates (n = 4). Values of individual experiments are indicated as dots. P-values were calculated using the one-way ANOVA (*P < 0.05; **P < 0.01).

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