Phospho-WASP (Tyr291) Antibody - #AF7304

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Product: Phospho-WASP (Tyr291) Antibody
Catalog: AF7304
Description: Rabbit polyclonal antibody to Phospho-WASP (Tyr291)
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Sheep, Rabbit, Dog, Xenopus
Mol.Wt.: 53kd; 53kD(Calculated).
Uniprot: P42768
RRID: AB_2843744

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Sheep(80%), Rabbit(100%), Dog(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
Phospho-WASP (Tyr291) Antibody detects endogenous levels of WASP only when phosphorylated at Tyr291.
RRID:
AB_2843744
Cite Format: Affinity Biosciences Cat# AF7304, RRID:AB_2843744.
Conjugate:
Unconjugated.
Purification:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Eczema thrombocytopenia; IMD2; SCNX; THC; THC1; Thrombocytopenia 1 (X linked); U42471; Was; WASp; WASP_HUMAN; Wiskott Aldrich syndrome (eczema thrombocytopenia); Wiskott Aldrich syndrome; Wiskott Aldrich syndrome protein; Wiskott-Aldrich syndrome protein;

Immunogens

Immunogen:
Uniprot:

P42768(WASP_HUMAN) >>Visit HPA database.

Gene(ID):
WAS(7454)
Expression:
P42768 WASP_HUMAN:

Expressed predominantly in the thymus. Also found, to a much lesser extent, in the spleen.

Sequence:
MSGGPMGGRPGGRGAPAVQQNIPSTLLQDHENQRLFEMLGRKCLTLATAVVQLYLALPPGAEHWTKEHCGAVCFVKDNPQKSYFIRLYGLQAGRLLWEQELYSQLVYSTPTPFFHTFAGDDCQAGLNFADEDEAQAFRALVQEKIQKRNQRQSGDRRQLPPPPTPANEERRGGLPPLPLHPGGDQGGPPVGPLSLGLATVDIQNPDITSSRYRGLPAPGPSPADKKRSGKKKISKADIGAPSGFKHVSHVGWDPQNGFDVNNLDPDLRSLFSRAGISEAQLTDAETSKLIYDFIEDQGGLEAVRQEMRRQEPLPPPPPPSRGGNQLPRPPIVGGNKGRSGPLPPVPLGIAPPPPTPRGPPPPGRGGPPPPPPPATGRSGPLPPPPPGAGGPPMPPPPPPPPPPPSSGNGPAPPPLPPALVPAGGLAPGGGRGALLDQIRQGIQLNKTPGAPESSALQPPPQSSEGLVGALMHVMQKRSRAIHSSDEGEDQAGDEDEDDEWDD

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Dog
100
Xenopus
100
Rabbit
100
Sheep
80
Pig
0
Horse
0
Bovine
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P42768 As Substrate

Site PTM Type Enzyme
S2 Phosphorylation
K76 Sumoylation
K76 Ubiquitination
K81 Ubiquitination
Y83 Phosphorylation
Y102 Phosphorylation Q9UM73 (ALK)
K144 Sumoylation
K144 Ubiquitination
K147 Sumoylation
K147 Ubiquitination
S221 Phosphorylation
S234 Phosphorylation
K235 Ubiquitination
S277 Phosphorylation Q07912 (TNK2)
T282 Phosphorylation
T286 Phosphorylation
S287 Phosphorylation
Y291 Phosphorylation P07948 (LYN) , P06241 (FYN) , P00519 (ABL1) , Q07912 (TNK2) , Q06187 (BTK) , P06239 (LCK) , P08631 (HCK)
S320 Phosphorylation
R321 Methylation
R328 Methylation
R338 Methylation
T355 Phosphorylation
R357 Methylation
R364 Methylation
R377 Methylation
R431 Methylation
R439 Methylation
S483 Phosphorylation P19784 (CSNK2A2) , P68400 (CSNK2A1)
S484 Phosphorylation P68400 (CSNK2A1) , P19784 (CSNK2A2)

Research Backgrounds

Function:

Effector protein for Rho-type GTPases that regulates actin filament reorganization via its interaction with the Arp2/3 complex. Important for efficient actin polymerization. Possible regulator of lymphocyte and platelet function. Mediates actin filament reorganization and the formation of actin pedestals upon infection by pathogenic bacteria. In addition to its role in the cytoplasmic cytoskeleton, also promotes actin polymerization in the nucleus, thereby regulating gene transcription and repair of damaged DNA. Promotes homologous recombination (HR) repair in response to DNA damage by promoting nuclear actin polymerization, leading to drive motility of double-strand breaks (DSBs).

PTMs:

Phosphorylated at Tyr-291 by FYN and HCK, inducing WAS effector activity after TCR engagement. Phosphorylation at Tyr-291 enhances WAS activity in promoting actin polymerization and filopodia formation.

Subcellular Location:

Cytoplasm>Cytoskeleton. Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed predominantly in the thymus. Also found, to a much lesser extent, in the spleen.

Subunit Structure:

Binds the Arp2/3 complex. Interacts with CDC42, RAC, NCK, HCK, FYN, SRC kinase FGR, BTK, ABL1, PSTPIP1, WIP, and to the p85 subunit of PLC-gamma. Interacts (via C-terminus) with ALDOA. Interacts with NCK1 (via SH3 domains) (By similarity). Interacts with FCHSD2 (By similarity).

(Microbial infection) Interacts with E.coli effector protein EspF(U).

Family&Domains:

The WH1 (Wasp homology 1) domain may bind a Pro-rich ligand.

The CRIB (Cdc42/Rac-interactive-binding) region binds to the C-terminal WH2 domain in the autoinhibited state of the protein. Binding of Rho-type GTPases to the CRIB induces a conformation change and leads to activation.

Research Fields

· Cellular Processes > Transport and catabolism > Endocytosis.   (View pathway)

· Cellular Processes > Cellular community - eukaryotes > Adherens junction.   (View pathway)

· Cellular Processes > Cellular community - eukaryotes > Tight junction.   (View pathway)

· Cellular Processes > Cell motility > Regulation of actin cytoskeleton.   (View pathway)

· Human Diseases > Infectious diseases: Bacterial > Bacterial invasion of epithelial cells.

· Human Diseases > Infectious diseases: Bacterial > Pathogenic Escherichia coli infection.

· Human Diseases > Infectious diseases: Bacterial > Shigellosis.

· Human Diseases > Infectious diseases: Bacterial > Salmonella infection.

· Human Diseases > Cancers: Overview > Choline metabolism in cancer.   (View pathway)

· Organismal Systems > Immune system > Chemokine signaling pathway.   (View pathway)

· Organismal Systems > Immune system > Fc gamma R-mediated phagocytosis.   (View pathway)

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