Product: Phospho-CDC25B (Ser353) Antibody
Catalog: AF3257
Description: Rabbit polyclonal antibody to Phospho-CDC25B (Ser353)
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Monkey
Prediction: Bovine, Horse, Sheep
Mol.Wt.: 64kDa; 65kD(Calculated).
Uniprot: P30305
RRID: AB_2834683

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 100ul $280 In stock
 200ul $350 In stock

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Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Monkey
Prediction:
Bovine(86%), Horse(100%), Sheep(86%)
Clonality:
Polyclonal
Specificity:
Phospho-CDC25B (Ser353) Antibody detects endogenous levels of CDC25B only when phosphorylated at Serine 353.
RRID:
AB_2834683
Cite Format: Affinity Biosciences Cat# AF3257, RRID:AB_2834683.
Conjugate:
Unconjugated.
Purification:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

AI604853; Cdc 25B; Cdc25b; CDC25HU2; Cdc25m2; Cell division cycle 25 homolog B; Cell division cycle 25B; Cell division cycle 25B isoform 1; Cell division cycle 25B isoform 2; Cell division cycle 25B isoform 3; Cell division cycle 25B isoform 4; Cell division cycle 25B isoform 5; Dual specificity phosphatase Cdc25B; EC 3.1.3.48; M phase inducer phosphatase 2; M-phase inducer phosphatase 2; MPIP2_HUMAN;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Description:
CDC25B is a member of the CDC25 family of phosphatases. CDC25B activates the cyclin dependent kinase CDC2 by removing two phosphate groups and it is required for entry into mitosis. CDC25B shuttles between the nucleus and the cytoplasm due to nuclear localization and nuclear export signals.
Sequence:
MEVPQPEPAPGSALSPAGVCGGAQRPGHLPGLLLGSHGLLGSPVRAAASSPVTTLTQTMHDLAGLGSETPKSQVGTLLFRSRSRLTHLSLSRRASESSLSSESSESSDAGLCMDSPSPMDPHMAEQTFEQAIQAASRIIRNEQFAIRRFQSMPVRLLGHSPVLRNITNSQAPDGRRKSEAGSGAASSSGEDKENDGFVFKMPWKPTHPSSTHALAEWASRREAFAQRPSSAPDLMCLSPDRKMEVEELSPLALGRFSLTPAEGDTEEDDGFVDILESDLKDDDAVPPGMESLISAPLVKTLEKEEEKDLVMYSKCQRLFRSPSMPCSVIRPILKRLERPQDRDTPVQNKRRRSVTPPEEQQEAEEPKARVLRSKSLCHDEIENLLDSDHRELIGDYSKAFLLQTVDGKHQDLKYISPETMVALLTGKFSNIVDKFVIVDCRYPYEYEGGHIKTAVNLPLERDAESFLLKSPIAPCSLDKRVILIFHCEFSSERGPRMCRFIRERDRAVNDYPSLYYPEMYILKGGYKEFFPQHPNFCEPQDYRPMNHEAFKDELKTFRLKTRSWAGERSRRELCSRLQDQ

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Bovine
86
Sheep
86
Pig
0
Dog
0
Xenopus
0
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P30305 As Substrate

Site PTM Type Enzyme
S15 Phosphorylation
S42 Phosphorylation
S50 Phosphorylation P53350 (PLK1)
T53 Phosphorylation
T54 Phosphorylation
T56 Phosphorylation
T58 Phosphorylation P53350 (PLK1)
T69 Phosphorylation
K71 Ubiquitination
T86 Phosphorylation
S89 Phosphorylation
S91 Phosphorylation
S97 Phosphorylation
S101 Phosphorylation
S115 Phosphorylation P45983 (MAPK8)
S117 Phosphorylation P45983 (MAPK8)
T127 Phosphorylation P53350 (PLK1)
S151 Phosphorylation P17612 (PRKACA)
S160 Phosphorylation P06493 (CDK1)
T167 Phosphorylation P53350 (PLK1)
S169 Phosphorylation Q14680 (MELK) , P49137 (MAPKAPK2)
K177 Ubiquitination
S178 Phosphorylation
S182 Phosphorylation
S186 Phosphorylation P68400 (CSNK2A1)
S187 Phosphorylation P68400 (CSNK2A1)
S188 Phosphorylation
K192 Ubiquitination
K200 Ubiquitination
K204 Ubiquitination
S209 Phosphorylation P53350 (PLK1)
S219 Phosphorylation Q14680 (MELK)
S229 Phosphorylation
S230 Phosphorylation P17612 (PRKACA) , O14757 (CHEK1)
S238 Phosphorylation
K242 Sumoylation
K242 Ubiquitination
S249 Phosphorylation Q15759 (MAPK11)
T265 Phosphorylation P53350 (PLK1)
S291 Phosphorylation P53350 (PLK1)
K299 Ubiquitination
K303 Ubiquitination
K307 Ubiquitination
Y312 Phosphorylation
S321 Phosphorylation P06493 (CDK1)
S323 Phosphorylation Q16539 (MAPK14) , P17612 (PRKACA) , P49137 (MAPKAPK2) , Q14680 (MELK)
S327 Phosphorylation
K334 Ubiquitination
T344 Phosphorylation
K349 Ubiquitination
S353 Phosphorylation P53350 (PLK1) , P49137 (MAPKAPK2) , Q15418 (RPS6KA1) , O14965 (AURKA) , P31749 (AKT1)
T355 Phosphorylation Q15418 (RPS6KA1)
K367 Sumoylation
K367 Ubiquitination
S373 Phosphorylation
K374 Ubiquitination
S375 Phosphorylation P49137 (MAPKAPK2) , Q8TDC3-2 (BRSK1) , Q16539 (MAPK14) , P53350 (PLK1)
S387 Phosphorylation
S397 Phosphorylation P53350 (PLK1)
K398 Ubiquitination
T404 Phosphorylation P53350 (PLK1)
K408 Ubiquitination
K413 Ubiquitination
K427 Ubiquitination
K434 Ubiquitination
K452 Ubiquitination
S465 Phosphorylation P53350 (PLK1)
K469 Ubiquitination
S470 Phosphorylation
K479 Ubiquitination
S513 Phosphorylation P53350 (PLK1)
K523 Ubiquitination
K527 Ubiquitination
K551 Ubiquitination
K555 Ubiquitination
T556 Phosphorylation
S563 Phosphorylation O14757 (CHEK1)

Research Backgrounds

Function:

Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Required for G2/M phases of the cell cycle progression and abscission during cytokinesis in a ECT2-dependent manner. Directly dephosphorylates CDK1 and stimulates its kinase activity. The three isoforms seem to have a different level of activity.

PTMs:

Phosphorylated by BRSK1 in vitro. Phosphorylated by CHEK1, which inhibits the activity of this protein. Phosphorylation at Ser-353 by AURKA might locally participate in the control of the onset of mitosis. Phosphorylation by MELK at Ser-169 promotes localization to the centrosome and the spindle poles during mitosis. Phosphorylation at Ser-323 and Ser-375 by MAPK14 is required for binding to 14-3-3 proteins.

Subcellular Location:

Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome. Cytoplasm>Cytoskeleton>Spindle pole.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Interacts with MAPK14 and 14-3-3 proteins.

Family&Domains:

Belongs to the MPI phosphatase family.

Research Fields

· Cellular Processes > Cell growth and death > Cell cycle.   (View pathway)

· Environmental Information Processing > Signal transduction > MAPK signaling pathway.   (View pathway)

· Human Diseases > Cancers: Overview > MicroRNAs in cancer.

· Organismal Systems > Endocrine system > Progesterone-mediated oocyte maturation.

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