Centaurin beta-1 Antibody | Affinity Biosciences

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Centaurin beta-1 Antibody - Western blot analysis of extracts from Mouse lung tissue sample,using Centaurin 1 Antibody(DF10313).

Product:	Centaurin beta-1 Antibody
Catalog:	DF10313
Description:	Rabbit polyclonal antibody to Centaurin beta-1
Application:	WB
Reactivity:	Human, Mouse, Rat
Mol.Wt.:	81KD; 82kD(Calculated).
Uniprot:	Q15027
RRID:	AB_2844320

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Clonality:

Polyclonal

Specificity:

Centaurin beta-1 Antibody detects endogenous levels of total Centaurin beta-1.

RRID:

AB_2844320
Cite Format: Affinity Biosciences Cat# DF10313, RRID:AB_2844320.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

ACAP 1; ACAP1; ACAP1_HUMAN; Arf GAP with coiled coil ANK repeat and PH domains 1; Arf GAP with coiled coil, ankyrin repeat, and pleckstrin homology domains 1; Arf-GAP with coiled-coil, ANK repeat and PH domain-containing protein 1; ArfGAP with coiled-coil, ankyrin repeat and PH domains 1; CENT B1; Centaurin beta 1; Centaurin-beta-1; CENTB 1; Cnt b1; Cnt-b1; Cntb1; KIAA0050;

Immunogens

Immunogen:

A synthesized peptide derived from human Centaurin beta-1, corresponding to a region within the internal amino acids.

Uniprot:

Q15027(ACAP1_HUMAN) >>Visit HPA database.

Gene(ID):

ACAP1(9744)

Expression:

Q15027 ACAP1_HUMAN:

Highest level in lung and spleen. Low level in heart, kidney, liver and pancreas.

Sequence:

Q15027 ACAP1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MTVKLDFEECLKDSPRFRASIELVEAEVSELETRLEKLLKLGTGLLESGRHYLAASRAFVVGICDLARLGPPEPMMAECLEKFTVSLNHKLDSHAELLDATQHTLQQQIQTLVKEGLRGFREARRDFWRGAESLEAALTHNAEVPRRRAQEAEEAGAALRTARAGYRGRALDYALQINVIEDKRKFDIMEFVLRLVEAQATHFQQGHEELSRLSQYRKELGAQLHQLVLNSAREKRDMEQRHVLLKQKELGGEEPEPSLREGPGGLVMEGHLFKRASNAFKTWSRRWFTIQSNQLVYQKKYKDPVTVVVDDLRLCTVKLCPDSERRFCFEVVSTSKSCLLQADSERLLQLWVSAVQSSIASAFSQARLDDSPRGPGQGSGHLAIGSAATLGSGGMARGREPGGVGHVVAQVQSVDGNAQCCDCREPAPEWASINLGVTLCIQCSGIHRSLGVHFSKVRSLTLDSWEPELVKLMCELGNVIINQIYEARVEAMAVKKPGPSCSRQEKEAWIHAKYVEKKFLTKLPEIRGRRGGRGRPRGQPPVPPKPSIRPRPGSLRSKPEPPSEDLGSLHPGALLFRASGHPPSLPTMADALAHGADVNWVNGGQDNATPLIQATAANSLLACEFLLQNGANVNQADSAGRGPLHHATILGHTGLACLFLKRGADLGARDSEGRDPLTIAMETANADIVTLLRLAKMREAEAAQGQAGDETYLDIFRDFSLMASDDPEKLSRRSHDLHTL

Research Backgrounds

Q15027_ACAP1_HUMAN

Function:

GTPase-activating protein (GAP) for ADP ribosylation factor 6 (ARF6) required for clathrin-dependent export of proteins from recycling endosomes to trans-Golgi network and cell surface. Required for regulated export of ITGB1 from recycling endosomes to the cell surface and ITGB1-dependent cell migration.

PTMs:

Phosphorylation at Ser-554 by PKB is required for interaction with ITGB1, export of ITGB1 from recycling endosomes to the cell surface and ITGB1-dependent cell migration.

Subcellular Location:

Recycling endosome membrane>Peripheral membrane protein>Cytoplasmic side.

Tissue Specificity:

Highest level in lung and spleen. Low level in heart, kidney, liver and pancreas.

Family&Domains:

PH domain binds phospholipids including phosphatidic acid, phosphatidylinositol 3-phosphate, phosphatidylinositol 3,5-bisphosphate (PIP2) and phosphatidylinositol 3,4,5-trisphosphate (PIP3). May mediate ACAP1-binding to PIP2 or PIP3 containing membranes. Only one PH domain of one ACAP1 dimer inserts into the membrane, while the other PH domain acts primaryly to interact with adjacent ACAP1 dimers.

The BAR domain mediates homodimerization, it can neither bind membrane nor impart curvature, but instead requires the neighboring PH domain to achieve these functions.

Research Fields

· Cellular Processes > Transport and catabolism > Endocytosis. (View pathway)

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Centaurin beta-1 Antibody - #DF10313