Phospho-HCLS1 (Tyr397) Antibody - #AF4407

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Product: Phospho-HCLS1 (Tyr397) Antibody
Catalog: AF4407
Description: Rabbit polyclonal antibody to Phospho-HCLS1 (Tyr397)
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Horse, Rabbit, Chicken
Mol.Wt.: 78kDa; 54kD(Calculated).
Uniprot: P14317
RRID: AB_2844472

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(92%), Zebrafish(80%), Horse(90%), Rabbit(82%), Chicken(88%)
Clonality:
Polyclonal
Specificity:
Phospho-HCLS1 (Tyr397) Antibody detects endogenous levels of HCLS1 only when phosphorylated at Tyr397.
RRID:
AB_2844472
Cite Format: Affinity Biosciences Cat# AF4407, RRID:AB_2844472.
Conjugate:
Unconjugated.
Purification:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Cortactin like; CTTNL; HCLS 1; Hcls1; HCLS1_HUMAN; Hematopoietic cell specific Lyn substrate 1; Hematopoietic cell-specific LYN substrate 1; Hematopoietic lineage cell-specific protein; HS 1; HS1; LckBP1; OTTHUMP00000215180; OTTHUMP00000215182; p75;

Immunogens

Immunogen:
Uniprot:

P14317(HCLS1_HUMAN) >>Visit HPA database.

Gene(ID):
HCLS1(3059)
Expression:
P14317 HCLS1_HUMAN:

Expressed only in tissues and cells of hematopoietic origin.

Sequence:
MWKSVVGHDVSVSVETQGDDWDTDPDFVNDISEKEQRWGAKTIEGSGRTEHINIHQLRNKVSEEHDVLRKKEMESGPKASHGYGGRFGVERDRMDKSAVGHEYVAEVEKHSSQTDAAKGFGGKYGVERDRADKSAVGFDYKGEVEKHTSQKDYSRGFGGRYGVEKDKWDKAALGYDYKGETEKHESQRDYAKGFGGQYGIQKDRVDKSAVGFNEMEAPTTAYKKTTPIEAASSGTRGLKAKFESMAEEKRKREEEEKAQQVARRQQERKAVTKRSPEAPQPVIAMEEPAVPAPLPKKISSEAWPPVGTPPSSESEPVRTSREHPVPLLPIRQTLPEDNEEPPALPPRTLEGLQVEEEPVYEAEPEPEPEPEPEPENDYEDVEEMDRHEQEDEPEGDYEEVLEPEDSSFSSALAGSSGCPAGAGAGAVALGISAVAVYDYQGEGSDELSFDPDDVITDIEMVDEGWWRGRCHGHFGLFPANYVKLLE

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
92
Horse
90
Chicken
88
Rabbit
82
Zebrafish
80
Bovine
75
Sheep
75
Dog
73
Xenopus
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P14317 As Substrate

Site PTM Type Enzyme
S13 Phosphorylation
T16 Phosphorylation P68400 (CSNK2A1)
T23 Phosphorylation P68400 (CSNK2A1)
S32 Phosphorylation
K34 Ubiquitination
K41 Acetylation
K41 Ubiquitination
T42 Phosphorylation
K60 Ubiquitination
S62 Phosphorylation
Y83 Phosphorylation
S97 Phosphorylation
Y103 Phosphorylation
K118 Ubiquitination
K123 Acetylation
K123 Ubiquitination
Y124 Phosphorylation
S134 Phosphorylation
Y140 Phosphorylation
K141 Acetylation
Y153 Phosphorylation
S154 Phosphorylation
R155 Methylation
Y161 Phosphorylation
K170 Ubiquitination
Y175 Phosphorylation
Y177 Phosphorylation
T181 Phosphorylation
S186 Phosphorylation
Y190 Phosphorylation
K192 Acetylation
K192 Ubiquitination
Y198 Phosphorylation
S208 Phosphorylation
T219 Phosphorylation
T220 Phosphorylation
Y222 Phosphorylation P07948 (LYN) , P09769 (FGR)
K223 Acetylation
K224 Acetylation
S232 Phosphorylation
K239 Ubiquitination
K241 Acetylation
T272 Phosphorylation
S275 Phosphorylation
T308 Phosphorylation
T333 Phosphorylation
Y360 Phosphorylation
Y378 Phosphorylation P07948 (LYN) , P43405 (SYK)
Y397 Phosphorylation P09769 (FGR) , P07948 (LYN) , P43405 (SYK)
Y481 Phosphorylation

Research Backgrounds

Function:

Substrate of the antigen receptor-coupled tyrosine kinase. Plays a role in antigen receptor signaling for both clonal expansion and deletion in lymphoid cells. May also be involved in the regulation of gene expression.

PTMs:

Phosphorylated by FES (By similarity). Phosphorylated by LYN, FYN and FGR after cross-linking of surface IgM on B-cells. Phosphorylation by LYN, FYN and FGR requires prior phosphorylation by SYK or FES.

Subcellular Location:

Membrane>Peripheral membrane protein. Cytoplasm. Mitochondrion.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed only in tissues and cells of hematopoietic origin.

Subunit Structure:

Associates with the SH2 and SH3 domains of LCK. Binding to he LCK SH3 domain occurs constitutively, while binding to the LCK SH2 domain occurs only upon TCR stimulation. A similar binding pattern was observed with LYN, but not with FYN in which the FYN SH2 region associates upon TCR stimulation but the FYN SH3 region does not associate regardless of TCR stimulation. Directly associates with HAX1, through binding to its C-terminal region. Interacts with HS1BP3. Interacts with FES/FPS (By similarity). Interacts (via SH2 domain) with FGR. Forms a multiprotein complex with LYN and ANKRD54 (By similarity).

Research Fields

· Cellular Processes > Cellular community - eukaryotes > Tight junction.   (View pathway)

· Human Diseases > Infectious diseases: Bacterial > Bacterial invasion of epithelial cells.

· Human Diseases > Infectious diseases: Bacterial > Pathogenic Escherichia coli infection.

· Human Diseases > Infectious diseases: Bacterial > Shigellosis.

· Human Diseases > Cancers: Overview > Proteoglycans in cancer.

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