SIN3A Antibody - #AF4612

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Product: SIN3A Antibody
Catalog: AF4612
Description: Rabbit polyclonal antibody to SIN3A
Application: WB
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 145kDa; 145kD(Calculated).
Uniprot: Q96ST3
RRID: AB_2844651

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(83%), Xenopus(86%)
Clonality:
Polyclonal
Specificity:
SIN3A Antibody detects endogenous levels of total SIN3A.
RRID:
AB_2844651
Cite Format: Affinity Biosciences Cat# AF4612, RRID:AB_2844651.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

AW553200; DKFZP434K2235; FLJ90319; Histone deacetylase complex subunit Sin 3a; Histone deacetylase complex subunit Sin3a; KIAA0700; Kiaa4126; mKIAA4126; Paired amphipathic helix protein Sin 3a; Paired amphipathic helix protein Sin3a; Sin 3a; SIN3 homolog A; SIN3 homolog A transcription regulator (yeast); SIN3 homolog A transcription regulator; SIN3 transcription regulator homolog A; Sin3a; SIN3A protein; SIN3A_HUMAN; Transcriptional co repressor Sin 3A; Transcriptional co repressor Sin3A; Transcriptional corepressor Sin3a; Transcriptional regulator SIN3A;

Immunogens

Immunogen:
Uniprot:

Q96ST3(SIN3A_HUMAN) >>Visit HPA database.

Gene(ID):
SIN3A(25942)
Expression:
Q96ST3 SIN3A_HUMAN:

Expressed in the developing brain, with highest levels of expression detected in the ventricular zone of various cortical regions.

Sequence:
MKRRLDDQESPVYAAQQRRIPGSTEAFPHQHRVLAPAPPVYEAVSETMQSATGIQYSVTPSYQVSAMPQSSGSHGPAIAAVHSSHHHPTAVQPHGGQVVQSHAHPAPPVAPVQGQQQFQRLKVEDALSYLDQVKLQFGSQPQVYNDFLDIMKEFKSQSIDTPGVISRVSQLFKGHPDLIMGFNTFLPPGYKIEVQTNDMVNVTTPGQVHQIPTHGIQPQPQPPPQHPSQPSAQSAPAPAQPAPQPPPAKVSKPSQLQAHTPASQQTPPLPPYASPRSPPVQPHTPVTISLGTAPSLQNNQPVEFNHAINYVNKIKNRFQGQPDIYKAFLEILHTYQKEQRNAKEAGGNYTPALTEQEVYAQVARLFKNQEDLLSEFGQFLPDANSSVLLSKTTAEKVDSVRNDHGGTVKKPQLNNKPQRPSQNGCQIRRHPTGTTPPVKKKPKLLNLKDSSMADASKHGGGTESLFFDKVRKALRSAEAYENFLRCLVIFNQEVISRAELVQLVSPFLGKFPELFNWFKNFLGYKESVHLETYPKERATEGIAMEIDYASCKRLGSSYRALPKSYQQPKCTGRTPLCKEVLNDTWVSFPSWSEDSTFVSSKKTQYEEHIYRCEDERFELDVVLETNLATIRVLEAIQKKLSRLSAEEQAKFRLDNTLGGTSEVIHRKALQRIYADKAADIIDGLRKNPSIAVPIVLKRLKMKEEEWREAQRGFNKVWREQNEKYYLKSLDHQGINFKQNDTKVLRSKSLLNEIESIYDERQEQATEENAGVPVGPHLSLAYEDKQILEDAAALIIHHVKRQTGIQKEDKYKIKQIMHHFIPDLLFAQRGDLSDVEEEEEEEMDVDEATGAVKKHNGVGGSPPKSKLLFSNTAAQKLRGMDEVYNLFYVNNNWYIFMRLHQILCLRLLRICSQAERQIEEENREREWEREVLGIKRDKSDSPAIQLRLKEPMDVDVEDYYPAFLDMVRSLLDGNIDSSQYEDSLREMFTIHAYIAFTMDKLIQSIVRQLQHIVSDEICVQVTDLYLAENNNGATGGQLNTQNSRSLLESTYQRKAEQLMSDENCFKLMFIQSQGQVQLTIELLDTEEENSDDPVEAERWSDYVERYMNSDTTSPELREHLAQKPVFLPRNLRRIRKCQRGREQQEKEGKEGNSKKTMENVDSLDKLECRFKLNSYKMVYVIKSEDYMYRRTALLRAHQSHERVSKRLHQRFQAWVDKWTKEHVPREMAAETSKWLMGEGLEGLVPCTTTCDTETLHFVSINKYRVKYGTVFKAP

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Xenopus
86
Chicken
83
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q96ST3 As Substrate

Site PTM Type Enzyme
S10 Phosphorylation
Y13 Phosphorylation
S23 Phosphorylation
Y41 Phosphorylation
K122 Ubiquitination
K155 Ubiquitination
S166 Phosphorylation
S169 Phosphorylation
T260 Phosphorylation
T266 Phosphorylation
Y272 Phosphorylation
S274 Phosphorylation
S277 Phosphorylation
T284 Phosphorylation
S289 Phosphorylation
Y310 Phosphorylation
K337 Ubiquitination
K343 Ubiquitination
T350 Phosphorylation
K391 Ubiquitination
S421 Phosphorylation
T432 Phosphorylation
T434 Phosphorylation O14757 (CHEK1)
T435 Phosphorylation
S450 Phosphorylation
S451 Phosphorylation
K457 Ubiquitination
K469 Acetylation
S505 Phosphorylation
Y524 Phosphorylation
T539 Phosphorylation
C551 S-Nitrosylation
Y558 Phosphorylation
K563 Ubiquitination
Y565 Phosphorylation
T574 Phosphorylation
T584 Phosphorylation
K601 Acetylation
K601 Ubiquitination
Y610 Phosphorylation
S641 Phosphorylation
K650 Ubiquitination
K676 Ubiquitination
K697 Ubiquitination
K715 Ubiquitination
K723 Acetylation
K727 Acetylation
K727 Ubiquitination
K737 Methylation
S746 Phosphorylation
K747 Ubiquitination
S748 Phosphorylation
Y757 Phosphorylation
T802 Phosphorylation
S832 Phosphorylation
S860 Phosphorylation
K865 Acetylation
K865 Ubiquitination
K875 Acetylation
K875 Ubiquitination
K934 Acetylation
K934 Ubiquitination
K937 Ubiquitination
S938 Phosphorylation
S940 Phosphorylation
Y958 Phosphorylation
Y959 Phosphorylation
K1053 Ubiquitination
S1089 Phosphorylation
Y1101 Phosphorylation
Y1105 Phosphorylation
S1108 Phosphorylation
T1110 Phosphorylation
T1111 Phosphorylation
S1112 Phosphorylation
K1122 Ubiquitination
K1154 Ubiquitination
S1161 Phosphorylation
K1164 Ubiquitination
K1170 Ubiquitination
Y1178 Phosphorylation
K1219 Ubiquitination
S1231 Phosphorylation
K1232 Ubiquitination
K1271 Ubiquitination

Research Backgrounds

Function:

Acts as a transcriptional repressor. Corepressor for REST. Interacts with MXI1 to repress MYC responsive genes and antagonize MYC oncogenic activities. Also interacts with MXD1-MAX heterodimers to repress transcription by tethering SIN3A to DNA. Acts cooperatively with OGT to repress transcription in parallel with histone deacetylation. Involved in the control of the circadian rhythms. Required for the transcriptional repression of circadian target genes, such as PER1, mediated by the large PER complex through histone deacetylation. Cooperates with FOXK1 to regulate cell cycle progression probably by repressing cell cycle inhibitor genes expression (By similarity). Required for cortical neuron differentiation and callosal axon elongation (By similarity).

PTMs:

SUMO1 sumoylated by TOPORS. Probably desumoylated by SENP2.

Subcellular Location:

Nucleus. Nucleus>Nucleolus.
Note: Recruited to the nucleolus by SAP30L.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in the developing brain, with highest levels of expression detected in the ventricular zone of various cortical regions.

Subunit Structure:

Interacts with ARID4B, BRMS1L, HCFC1, HDAC1, HDAC2, MXI1, SAP30L, SAP130, SFPQ and TOPORS. Interacts with OGT (via TPRs 1-6); the interaction mediates transcriptional repression in parallel with histone deacetylase. Interacts with BAZ2A, MXD1, MXD3, MXD4, MBD2, DACH1, NCOR1, NR4A2, REST, RLIM, SAP30, SETDB1, SMYD2, and SUDS3. Interacts with PHF12 in a complex composed of HDAC1, PHF12 and SAP30 (By similarity). Interacts with TET1; the interaction recruits SIN3A to gene promoters. The large PER complex involved in the histone deacetylation is composed of at least HDAC1, PER2, SFPQ and SIN3A (By similarity). Interacts with KLF11 (By similarity). Interacts with PPHLN1. Found in a complex with YY1, GON4L and HDAC1 (By similarity). Interacts (via PAH2) with FOXK1 (By similarity). Interacts with FOXK2 (By similarity). Found in a complex composed of at least SINHCAF, SIN3A, HDAC1, SAP30, RBBP4, OGT and TET1. Interacts with SINHCAF (By similarity). Interacts with SPHK2.

Research Fields

· Human Diseases > Neurodegenerative diseases > Huntington's disease.

· Human Diseases > Cancers: Overview > Transcriptional misregulation in cancer.

· Organismal Systems > Endocrine system > Thyroid hormone signaling pathway.   (View pathway)

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