Product: Phospho-LIMK2 (Ser283) Antibody
Catalog: AF3346
Description: Rabbit polyclonal antibody to Phospho-LIMK2 (Ser283)
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.: 72kDa; 72kD(Calculated).
Uniprot: P53671
RRID: AB_2834761

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Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Zebrafish(91%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%)
Clonality:
Polyclonal
Specificity:
Phospho-LIMK2 (Ser283) Antibody detects endogenous levels of LIMK2 only when phosphorylated at Serine 283.
RRID:
AB_2834761
Cite Format: Affinity Biosciences Cat# AF3346, RRID:AB_2834761.
Conjugate:
Unconjugated.
Purification:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

LIM containing protein kinase 2b; LIM domain kinase 2; LIM domain kinase 2b; LIM kinase 2b; LIM motif containing protein kinase 2b; LIMK 2b; LIMK-2; Limk2; LIMK2 b; LIMK2_HUMAN; LIMK2b;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
P53671 LIMK2_HUMAN:

Highest expression in the placenta; moderate level in liver, lung, kidney, and pancreas. LIMK2a is found to be more abundant then LIMK2b in liver, colon, stomach, and spleen, while in brain, kidney, and placenta LIMK2b is the dominant form. In adult lung, both LIMK2a and LIMK2b is nearly equally observed.

Description:
There are approximately 40 known eukaryotic LIM proteins, so named for the LIM domains they contain. LIM domains are highly conserved cysteine-rich structures containing 2 zinc fingers.
Sequence:
MSALAGEDVWRCPGCGDHIAPSQIWYRTVNETWHGSCFRCSECQDSLTNWYYEKDGKLYCPKDYWGKFGEFCHGCSLLMTGPFMVAGEFKYHPECFACMSCKVIIEDGDAYALVQHATLYCGKCHNEVVLAPMFERLSTESVQEQLPYSVTLISMPATTEGRRGFSVSVESACSNYATTVQVKEVNRMHISPNNRNAIHPGDRILEINGTPVRTLRVEEVEDAISQTSQTLQLLIEHDPVSQRLDQLRLEARLAPHMQNAGHPHALSTLDTKENLEGTLRRRSLRRSNSISKSPGPSSPKEPLLFSRDISRSESLRCSSSYSQQIFRPCDLIHGEVLGKGFFGQAIKVTHKATGKVMVMKELIRCDEETQKTFLTEVKVMRSLDHPNVLKFIGVLYKDKKLNLLTEYIEGGTLKDFLRSMDPFPWQQKVRFAKGIASGMAYLHSMCIIHRDLNSHNCLIKLDKTVVVADFGLSRLIVEERKRAPMEKATTKKRTLRKNDRKKRYTVVGNPYWMAPEMLNGKSYDETVDIFSFGIVLCEIIGQVYADPDCLPRTLDFGLNVKLFWEKFVPTDCPPAFFPLAAICCRLEPESRPAFSKLEDSFEALSLYLGELGIPLPAELEELDHTVSMQYGLTRDSPP

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Chicken
100
Rabbit
100
Zebrafish
91
Xenopus
67
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P53671 As Substrate

Site PTM Type Enzyme
K57 Ubiquitination
K62 Ubiquitination
S149 Phosphorylation
T151 Phosphorylation
S166 Phosphorylation
S168 Phosphorylation
T179 Phosphorylation
S191 Phosphorylation
T210 Phosphorylation
K272 Ubiquitination
T278 Phosphorylation
S283 Phosphorylation O14965 (AURKA) , Q05655 (PRKCD)
S287 Phosphorylation
S289 Phosphorylation
S291 Phosphorylation
K292 Ubiquitination
S293 Phosphorylation
S297 Phosphorylation
S298 Phosphorylation
K300 Ubiquitination
S310 Phosphorylation
S318 Phosphorylation
K347 Ubiquitination
K355 Acetylation
K355 Ubiquitination
K360 Ubiquitination
K371 Ubiquitination
K378 Ubiquitination
K414 Ubiquitination
K428 Ubiquitination
K487 Ubiquitination
T494 Phosphorylation O14965 (AURKA)
T505 Phosphorylation Q5VT25 (CDC42BPA) , O14965 (AURKA) , Q13464 (ROCK1) , O75116 (ROCK2)

PTMs - P53671 As Enzyme

Substrate Site Source
P23528 (CFL1) S3 Uniprot
P60981 (DSTN) S3 Uniprot

Research Backgrounds

Function:

Serine/threonine-protein kinase that plays an essential role in the regulation of actin filament dynamics. Acts downstream of several Rho family GTPase signal transduction pathways. Involved in astral microtubule organization and mitotic spindle orientation during early stage of mitosis by mediating phosphorylation of TPPP. Displays serine/threonine-specific phosphorylation of myelin basic protein and histone (MBP) in vitro.

PTMs:

Phosphorylated on serine and/or threonine residues by ROCK1.

Subcellular Location:

Cytoplasm. Nucleus. Cytoplasm>Cytoskeleton>Spindle.

Cytoplasm. Nucleus.
Note: Isoform LIMK2a is distributed in the cytoplasm and the nucleus.

Cytoplasm. Nucleus.
Note: Isoform LIMK2b occurs mainly in the cytoplasm and is scarcely translocated to the nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Highest expression in the placenta; moderate level in liver, lung, kidney, and pancreas. LIMK2a is found to be more abundant then LIMK2b in liver, colon, stomach, and spleen, while in brain, kidney, and placenta LIMK2b is the dominant form. In adult lung, both LIMK2a and LIMK2b is nearly equally observed.

Subunit Structure:

Binds ROCK1 and MARF1 (Ref.9,. Interacts with NISCH (By similarity).

Family&Domains:

Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.

Research Fields

· Cellular Processes > Cell motility > Regulation of actin cytoskeleton.   (View pathway)

· Organismal Systems > Development > Axon guidance.   (View pathway)

· Organismal Systems > Immune system > Fc gamma R-mediated phagocytosis.   (View pathway)

References

1). Deng Z et al. Function of connexin 43 and RhoA/LIMK2/Cofilin signaling pathway in transient changes of contraction and dilation of human umbilical arterial smooth muscle cells. The International Journal of Biochemistry & Cell Biology 2022 Oct 28;153:106326 (PubMed: 36330887) [IF=4.0]

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