Spartin, SPG20 Antibody - #DF12086

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Product: Spartin, SPG20 Antibody
Catalog: DF12086
Description: Rabbit polyclonal antibody to Spartin, SPG20
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Chicken, Xenopus
Mol.Wt.: 75 kDa,84 kDa; 73kD(Calculated).
Uniprot: Q8N0X7
RRID: AB_2844891

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(92%), Bovine(100%), Horse(92%), Sheep(92%), Rabbit(92%), Chicken(83%), Xenopus(83%)
Clonality:
Polyclonal
Specificity:
Spartin, SPG20 Antibody detects endogenous levels of total Spartin, SPG20.
RRID:
AB_2844891
Cite Format: Affinity Biosciences Cat# DF12086, RRID:AB_2844891.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

SPARTIN; Spastic paraplegia 20 (Troyer syndrome); TAHCCP1; Trans activated by hepatitis C virus core protein 1;

Immunogens

Immunogen:

A synthesized peptide derived from human Spartin, SPG20, corresponding to a region within the internal amino acids.

Uniprot:

Q8N0X7(SPART_HUMAN) >>Visit HPA database.

Gene(ID):
SPART(23111)
Expression:
Q8N0X7 SPART_HUMAN:

Ubiquitously expressed, with highest levels of expression detected in adipose tissue.

Sequence:
MEQEPQNGEPAEIKIIREAYKKAFLFVNKGLNTDELGQKEEAKNYYKQGIGHLLRGISISSKESEHTGPGWESARQMQQKMKETLQNVRTRLEILEKGLATSLQNDLQEVPKLYPEFPPKDMCEKLPEPQSFSSAPQHAEVNGNTSTPSAGAVAAPASLSLPSQSCPAEAPPAYTPQAAEGHYTVSYGTDSGEFSSVGEEFYRNHSQPPPLETLGLDADELILIPNGVQIFFVNPAGEVSAPSYPGYLRIVRFLDNSLDTVLNRPPGFLQVCDWLYPLVPDRSPVLKCTAGAYMFPDTMLQAAGCFVGVVLSSELPEDDRELFEDLLRQMSDLRLQANWNRAEEENEFQIPGRTRPSSDQLKEASGTDVKQLDQGNKDVRHKGKRGKRAKDTSSEEVNLSHIVPCEPVPEEKPKELPEWSEKVAHNILSGASWVSWGLVKGAEITGKAIQKGASKLRERIQPEEKPVEVSPAVTKGLYIAKQATGGAAKVSQFLVDGVCTVANCVGKELAPHVKKHGSKLVPESLKKDKDGKSPLDGAMVVAASSVQGFSTVWQGLECAAKCIVNNVSAETVQTVRYKYGYNAGEATHHAVDSAVNVGVTAYNINNIGIKAMVKKTATQTGHTLLEDYQIVDNSQRENQEGAANVNVRGEKDEQTKEVKEAKKKDK

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Bovine
100
Pig
92
Horse
92
Sheep
92
Rabbit
92
Xenopus
83
Chicken
83
Dog
79
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

Research Backgrounds

Function:

May be implicated in endosomal trafficking, or microtubule dynamics, or both. Participates in cytokinesis.

PTMs:

Ubiquitinated; ubiquitination does not require ITCH and WWP1.

Subcellular Location:

Cytoplasm. Midbody.
Note: Transiently associated with endosomes (PubMed:19580544). Colocalized with IST1 to the ends of Flemming bodies during cytokinesis (PubMed:20719964).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Ubiquitously expressed, with highest levels of expression detected in adipose tissue.

Research Fields

· Cellular Processes > Transport and catabolism > Endocytosis.   (View pathway)

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