Product: HSP27 Antibody
Catalog: AF6082
Description: Rabbit polyclonal antibody to HSP27
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Rabbit, Dog, Xenopus
Mol.Wt.: 27kDa; 23kD(Calculated).
Uniprot: P04792
RRID: AB_2834851

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 100ul $280 In stock
 200ul $350 In stock

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Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(91%), Zebrafish(91%), Bovine(100%), Horse(100%), Rabbit(100%), Dog(100%), Xenopus(91%)
Clonality:
Polyclonal
Specificity:
HSP27 Antibody detects endogenous levels of total HSP27.
RRID:
AB_2834851
Cite Format: Affinity Biosciences Cat# AF6082, RRID:AB_2834851.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Heat shock 27kDa protein; 28 kDa heat shock protein; CMT2F; DKFZp586P1322; epididymis secretory protein Li 102; Estrogen regulated 24 kDa protein; Estrogen-regulated 24 kDa protein; Heat shock 25kDa protein 1; Heat shock 27 kDa protein; Heat shock 27kD protein 1; Heat shock 27kDa protein 1; Heat shock 28kDa protein 1; Heat Shock Protein 27; Heat shock protein beta 1; Heat shock protein beta-1; heat shock protein family B (small) member 1; HEL-S-102; HMN2B; HS.76067; Hsp 25; HSP 27; Hsp 28; Hsp B1; Hsp25; HSP27; Hsp28; HspB1; HSPB1_HUMAN; SRP27; Stress responsive protein 27; Stress-responsive protein 27;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
P04792 HSPB1_HUMAN:

Detected in all tissues tested: skeletal muscle, heart, aorta, large intestine, small intestine, stomach, esophagus, bladder, adrenal gland, thyroid, pancreas, testis, adipose tissue, kidney, liver, spleen, cerebral cortex, blood serum and cerebrospinal fluid. Highest levels are found in the heart and in tissues composed of striated and smooth muscle.

Description:
HSP27 is a small heat shock protein that is regulated both transcriptionally and posttranslationally. Modulates actin polymerization and reorganization.
Sequence:
MTERRVPFSLLRGPSWDPFRDWYPHSRLFDQAFGLPRLPEEWSQWLGGSSWPGYVRPLPPAAIESPAVAAPAYSRALSRQLSSGVSEIRHTADRWRVSLDVNHFAPDELTVKTKDGVVEITGKHEERQDEHGYISRCFTRKYTLPPGVDPTQVSSSLSPEGTLTVEAPMPKLATQSNEITIPVTFESRAQLGGPEAAKSDETAAK

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Bovine
100
Dog
100
Rabbit
100
Pig
91
Xenopus
91
Zebrafish
91
Sheep
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P04792 As Substrate

Site PTM Type Enzyme
R5 Methylation
S9 Phosphorylation
R12 Methylation
S15 Phosphorylation P49137 (MAPKAPK2) , Q05655 (PRKCD) , Q8IW41 (MAPKAPK5) , Q13976-2 (PRKG1) , Q16644 (MAPKAPK3) , P23443 (RPS6KB1)
Y23 Phosphorylation
S26 Phosphorylation
R37 Methylation
Y54 Phosphorylation
R56 Methylation
S65 Phosphorylation
Y73 Phosphorylation
S74 Phosphorylation
S78 Phosphorylation Q13237 (PRKG2) , Q13976 (PRKG1) , P23443 (RPS6KB1) , P17612 (PRKACA) , Q9UBS0 (RPS6KB2) , Q16644 (MAPKAPK3) , P49137 (MAPKAPK2) , Q8IW41 (MAPKAPK5) , P51812 (RPS6KA3)
S82 Phosphorylation P23443 (RPS6KB1) , P17612 (PRKACA) , Q8IW41 (MAPKAPK5) , P51812 (RPS6KA3) , P98161 (PKD1) , P49137 (MAPKAPK2) , Q9Y243 (AKT3) , Q9UBS0 (RPS6KB2) , Q13976 (PRKG1) , Q13237 (PRKG2) , P31751 (AKT2) , P31749 (AKT1) , Q16644 (MAPKAPK3) , Q15139 (PRKD1)
S83 Phosphorylation
S86 Phosphorylation Q05655 (PRKCD)
R89 Methylation
T91 Phosphorylation
S98 Phosphorylation
T110 Phosphorylation
K112 Ubiquitination
T113 Phosphorylation
K114 Sumoylation
K114 Ubiquitination
T121 Phosphorylation
K123 Acetylation
K123 Ubiquitination
Y133 Phosphorylation
S135 Phosphorylation
T139 Phosphorylation
K141 Ubiquitination
Y142 Phosphorylation
T143 Phosphorylation P17612 (PRKACA) , Q13976 (PRKG1) , Q13237 (PRKG2)
T162 Phosphorylation
T164 Phosphorylation
K171 Ubiquitination
T174 Phosphorylation
S176 Phosphorylation Q16539 (MAPK14)
T180 Phosphorylation
T184 Phosphorylation
S187 Phosphorylation
K198 Sumoylation
K198 Ubiquitination
S199 Phosphorylation
T202 Phosphorylation
K205 Acetylation
K205 Ubiquitination

Research Backgrounds

Function:

Small heat shock protein which functions as a molecular chaperone probably maintaining denatured proteins in a folding-competent state. Plays a role in stress resistance and actin organization. Through its molecular chaperone activity may regulate numerous biological processes including the phosphorylation and the axonal transport of neurofilament proteins.

PTMs:

Phosphorylated upon exposure to protein kinase C activators and heat shock. Phosphorylation by MAPKAPK2 and MAPKAPK3 in response to stress dissociates HSPB1 from large small heat-shock protein (sHsps) oligomers and impairs its chaperone activity and ability to protect against oxidative stress effectively. Phosphorylation by MAPKAPK5 in response to PKA stimulation induces F-actin rearrangement.

Subcellular Location:

Cytoplasm. Nucleus. Cytoplasm>Cytoskeleton>Spindle.
Note: Cytoplasmic in interphase cells. Colocalizes with mitotic spindles in mitotic cells. Translocates to the nucleus during heat shock and resides in sub-nuclear structures known as SC35 speckles or nuclear splicing speckles.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Detected in all tissues tested: skeletal muscle, heart, aorta, large intestine, small intestine, stomach, esophagus, bladder, adrenal gland, thyroid, pancreas, testis, adipose tissue, kidney, liver, spleen, cerebral cortex, blood serum and cerebrospinal fluid. Highest levels are found in the heart and in tissues composed of striated and smooth muscle.

Subunit Structure:

Homooligomer. Homodimer; becomes monomeric upon activation. Heterooligomer; with HSPB6. Associates with alpha- and beta-tubulin. Interacts with TGFB1I1 (By similarity). Interacts with CRYAB. Interacts with HSPB8. Interacts with HSPBAP1.

Family&Domains:

Belongs to the small heat shock protein (HSP20) family.

Research Fields

· Environmental Information Processing > Signal transduction > MAPK signaling pathway.   (View pathway)

· Human Diseases > Infectious diseases: Parasitic > Amoebiasis.

· Human Diseases > Infectious diseases: Viral > Epstein-Barr virus infection.

References

1). Xia F et al. Isobaric Tags for Relative and Absolute Quantitation (iTRAQ)-Based Proteomic Analysis of Hugan Qingzhi and Its Protective Properties against Free Fatty Acid-Induced L02 Hepatocyte Injury. Frontiers in Pharmacology 2017 Feb 28;8:99 (PubMed: 28293193) [IF=5.6]

Application: WB    Species: human    Sample:

FIGURE 6 | Effects of HQT-medicated serum on amount of phosphorylated HSP27 and total HSP27 (A,B) and phosphorylation level of HSP27 (A,C) in FFA-stimulated L02 hepatocytes over 24 h. GAPDH was used as a loading control. Values are expressed as mean ± SD in each group. ∗∗p < 0.01 vs. control group; ##p < 0.01 vs. FFA group.

2). Zhou Z et al. Exosomes derived from dental pulp stem cells accelerate cutaneous wound healing by enhancing angiogenesis via the Cdc42/p38 MAPK pathway. International Journal of Molecular Medicine 2022 Dec;50(6) (PubMed: 36321793) [IF=5.4]

Application: WB    Species: Human    Sample:

Figure 4 - DPSC-Exos promote angiogenesis via p38 MAPK to promote wound healing. (A and B) HUVECs were treated with DPSC-Exos in the presence or absence of SB203580. The expression of p38, p-p38, hsp27 and p-hsp27 was detected using western blot analysis (n=3). (C and D) The migration of HUVECs stimulated by DPSC-Exos or an equal volume of PBS in the presence or absence of SB203580 (n=3). Scale bar, 100 µm. (E and F) Representative images of scratch wound assay in HUVECs treated with DPSC-Exos or PBS in the presence or absence of SB203580 (n=3). Scale bar, 100 µm. (G) The proliferation of HUVECs receiving different treatments were examined using CCK-8 assay (n=3). (H-J) Representative images of the tube formation assay on Matrigel in HUVECs treated with DPSC-Exos or PBS (n=3). Scale bar, 100 µm. *P

3). Liu Y et al. A Group of Complexes Based on PAMAM and Quantum Dots Used in Clinical Immunoassays. Nanoscale Research Letters 2020 Apr 3;15(1):71 (PubMed: 32246298)

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Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

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