Product: APPBP1 Antibody
Catalog: DF12827
Description: Rabbit polyclonal antibody to APPBP1
Application: WB
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.: 60 kDa; 60kD(Calculated).
Uniprot: Q13564
RRID: AB_2845788

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Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Zebrafish(80%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(90%), Dog(100%), Chicken(90%)
Clonality:
Polyclonal
Specificity:
APPBP1 Antibody detects endogenous levels of total APPBP1.
RRID:
AB_2845788
Cite Format: Affinity Biosciences Cat# DF12827, RRID:AB_2845788.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

59 kDa; A 116A10.1; Amyloid beta precursor protein binding protein 1 59kD; Amyloid beta precursor protein binding protein 1 59kDa; Amyloid beta precursor protein binding protein 1; Amyloid beta precursor protein binding protein 1, 59 kDa; Amyloid beta precursor protein-binding protein 1; Amyloid protein binding protein 1; Amyloid protein-binding protein 1; APP BP1; APP-BP1; APPBP 1; APPBP1; HPP 1; HPP1; NAE 1; NAE1; NEDD8 activating enzyme E1 regulatory subunit; NEDD8 activating enzyme E1 subunit 1; NEDD8 activating enzyme E1 subunit; NEDD8-activating enzyme E1 regulatory subunit; Proto oncogene protein 1; Proto-oncogene protein 1; Protooncogene protein 1; ula 1; ULA1_HUMAN;

Immunogens

Immunogen:

A synthesized peptide derived from human APPBP1, corresponding to a region within N-terminal amino acids.

Uniprot:
Gene(ID):
Expression:
Q13564 ULA1_HUMAN:

Ubiquitous in fetal tissues. Expressed throughout the adult brain.

Sequence:
MAQLGKLLKEQKYDRQLRLWGDHGQEALESAHVCLINATATGTEILKNLVLPGIGSFTIIDGNQVSGEDAGNNFFLQRSSIGKNRAEAAMEFLQELNSDVSGSFVEESPENLLDNDPSFFCRFTVVVATQLPESTSLRLADVLWNSQIPLLICRTYGLVGYMRIIIKEHPVIESHPDNALEDLRLDKPFPELREHFQSYDLDHMEKKDHSHTPWIVIIAKYLAQWYSETNGRIPKTYKEKEDFRDLIRQGILKNENGAPEDEENFEEAIKNVNTALNTTQIPSSIEDIFNDDRCINITKQTPSFWILARALKEFVAKEGQGNLPVRGTIPDMIADSGKYIKLQNVYREKAKKDAAAVGNHVAKLLQSIGQAPESISEKELKLLCSNSAFLRVVRCRSLAEEYGLDTINKDEIISSMDNPDNEIVLYLMLRAVDRFHKQQGRYPGVSNYQVEEDIGKLKSCLTGFLQEYGLSVMVKDDYVHEFCRYGAAEPHTIAAFLGGAAAQEVIKIITKQFVIFNNTYIYSGMSQTSATFQL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Chicken
90
Rabbit
90
Zebrafish
80
Xenopus
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

Research Backgrounds

Function:

Regulatory subunit of the dimeric UBA3-NAE1 E1 enzyme. E1 activates NEDD8 by first adenylating its C-terminal glycine residue with ATP, thereafter linking this residue to the side chain of the catalytic cysteine, yielding a NEDD8-UBA3 thioester and free AMP. E1 finally transfers NEDD8 to the catalytic cysteine of UBE2M. Necessary for cell cycle progression through the S-M checkpoint. Overexpression of NAE1 causes apoptosis through deregulation of NEDD8 conjugation.

PTMs:

Ubiquitinated by TRIP12, leading to its degradation by the proteasome.

Subcellular Location:

Cell membrane.
Note: Colocalizes with APP in lipid rafts.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Ubiquitous in fetal tissues. Expressed throughout the adult brain.

Family&Domains:

Belongs to the ubiquitin-activating E1 family. ULA1 subfamily.

Research Fields

· Human Diseases > Neurodegenerative diseases > Alzheimer's disease.

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