P2X2 Antibody | Affinity Biosciences

WB
IHC
IF/ICC
Cites

1/5

Western blot analysis of extracts from HepG2 cells , using P2X2 Antibody.

DF13236 at 1/100 staining Human kidney cancer and adjacent normal tissues by IHC-P.

DF13236 at 1/100 staining Mouse brain tissue by IHC-P.

DF13236 staining Hela cells by IF/ICC.

Figure 9 P2RX2 acts as a tumor suppressor in PCa: (a) The expression of P2RX2 in C4-2b and CWR22Rv1 cells after transfection.

Product:	P2X2 Antibody
Catalog:	DF13236
Description:	Rabbit polyclonal antibody to P2X2
Application:	WB IHC IF/ICC
Reactivity:	Human, Mouse
Mol.Wt.:	51kDa; 52kD(Calculated).
Uniprot:	Q9UBL9
RRID:	AB_2846255

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

IF/ICC 1:100-1:500, WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse

Clonality:

Polyclonal

Specificity:

P2X2 Antibody detects endogenous levels of total P2X2.

RRID:

AB_2846255
Cite Format: Affinity Biosciences Cat# DF13236, RRID:AB_2846255.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

ATP receptor; MGC129601; P2RX 2; P2RX2; P2RX2_HUMAN; P2X purinoceptor 2; P2X Receptor subunit 2; P2X2; P2X2a; Purinergic receptor; Purinergic receptor P2X ligand gated ion channel 2; Purinergic receptor P2X2 isoform A; Purinergic receptor P2X2 isoform B; Purinergic receptor P2X2 isoform C; Purinergic receptor P2X2 isoform D; Purinergic receptor P2X2 isoform H; Purinergic receptor P2X2 isoform I;

Immunogens

Immunogen:

Uniprot:

Q9UBL9(P2RX2_HUMAN) >>Visit HPA database.

Gene(ID):

P2RX2(22953)

Sequence:

Q9UBL9 P2RX2_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MAAAQPKYPAGATARRLARGCWSALWDYETPKVIVVRNRRLGVLYRAVQLLILLYFVWYVFIVQKSYQESETGPESSIITKVKGITTSEHKVWDVEEYVKPPEGGSVFSIITRVEATHSQTQGTCPESIRVHNATCLSDADCVAGELDMLGNGLRTGRCVPYYQGPSKTCEVFGWCPVEDGASVSQFLGTMAPNFTILIKNSIHYPKFHFSKGNIADRTDGYLKRCTFHEASDLYCPIFKLGFIVEKAGESFTELAHKGGVIGVIINWDCDLDLPASECNPKYSFRRLDPKHVPASSGYNFRFAKYYKINGTTTRTLIKAYGIRIDVIVHGQAGKFSLIPTIINLATALTSVGVGSFLCDWILLTFMNKNKVYSHKKFDKVCTPSHPSGSWPVTLARVLGQAPPEPGHRSEDQHPSPPSGQEGQQGAECGPAFPPLRPCPISAPSEQMVDTPASEPAQASTPTDPKGLAQL

PTMs - Q9UBL9 As Substrate

Q9UBL9

Site	PTM Type	Source
K83	Ubiquitination	Uniprot
Y222	Phosphorylation	Uniprot
Y307	Phosphorylation	Uniprot
T312	Phosphorylation	Uniprot

Research Backgrounds

Q9UBL9_P2RX2_HUMAN

Function:

Ion channel gated by extracellular ATP involved in a variety of cellular responses, such as excitatory postsynaptic responses in sensory neurons, neuromuscular junctions (NMJ) formation, hearing, perception of taste and peristalsis. In the inner ear, regulates sound transduction and auditory neurotransmission, outer hair cell electromotility, inner ear gap junctions, and K(+) recycling. Mediates synaptic transmission between neurons and from neurons to smooth muscle.

Subcellular Location:

Cell membrane>Multi-pass membrane protein.
Note: Localizes to the apical membranes of hair cells in the organ of Corti.

Subunit Structure:

Homotrimer and heterotrimer; functional P2XRs are organized as homomeric and heteromeric trimers.

Family&Domains:

Belongs to the P2X receptor family.

Research Fields

· Environmental Information Processing > Signal transduction > Calcium signaling pathway. (View pathway)

· Environmental Information Processing > Signaling molecules and interaction > Neuroactive ligand-receptor interaction.

· Organismal Systems > Sensory system > Taste transduction.

References

1). Identification of Calcium Channel-Related Gene P2RX2 for Prognosis and Immune Infiltration in Prostate Cancer. Disease Markers (PubMed: 36246559)

Application: WB Species: Human Sample: C4-2b and CWR22Rv1 cells

Figure 9 P2RX2 acts as a tumor suppressor in PCa: (a) The expression of P2RX2 in C4-2b and CWR22Rv1 cells after transfection. (b) Cell counting kit-8 for C4-2b and CWR22Rv1 cells after 24 h. (c–d) Scratch assay in the C4-2b and CWR22Rv1 cells. (f–g) Colony formation in C4-2b and CWR22Rv1 cells.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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P2X2 Antibody - #DF13236