PP2C alpha Antibody - #DF13395

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Product: PP2C alpha Antibody
Catalog: DF13395
Description: Rabbit polyclonal antibody to PP2C alpha
Application: WB IF/ICC
Reactivity: Human
Prediction: Pig, Bovine, Horse, Rabbit, Dog
Mol.Wt.: 52kDa; 53kD(Calculated).
Uniprot: O75688
RRID: AB_2846414

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
IF/ICC 1:100-1:500, WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Prediction:
Pig(100%), Bovine(100%), Horse(86%), Rabbit(100%), Dog(100%)
Clonality:
Polyclonal
Specificity:
PP2C alpha Antibody detects endogenous levels of total PP2C alpha.
RRID:
AB_2846414
Cite Format: Affinity Biosciences Cat# DF13395, RRID:AB_2846414.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

MGC21657; OTTHUMP00000158953; OTTHUMP00000158954; OTTHUMP00000158955; OTTHUMP00000202394; OTTHUMP00000202395; OTTHUMP00000202399; PP2C beta; PP2C beta X; PP2C-beta; PP2CB; PP2Cbeta; PPC2BETAX; PPM 1B; Ppm1b; PPM1B_HUMAN; Protein phosphatase 1B (formerly 2C) magnesium dependent beta isoform; Protein phosphatase 1B; Protein phosphatase 1B magnesium dependent beta isoform; Protein phosphatase 2C beta isoform; Protein phosphatase 2C isoform beta; Protein phosphatase 2C like protein; Protein phosphatase, Mg2+/Mn2+ dependent, 1B;

Immunogens

Immunogen:
Uniprot:

O75688(PPM1B_HUMAN) >>Visit HPA database.

Gene(ID):
PPM1B(5495)
Expression:
O75688 PPM1B_HUMAN:

Highly expressed in heart and skeletal muscle.

Sequence:
MGAFLDKPKTEKHNAHGAGNGLRYGLSSMQGWRVEMEDAHTAVVGIPHGLEDWSFFAVYDGHAGSRVANYCSTHLLEHITTNEDFRAAGKSGSALELSVENVKNGIRTGFLKIDEYMRNFSDLRNGMDRSGSTAVGVMISPKHIYFINCGDSRAVLYRNGQVCFSTQDHKPCNPREKERIQNAGGSVMIQRVNGSLAVSRALGDYDYKCVDGKGPTEQLVSPEPEVYEILRAEEDEFIILACDGIWDVMSNEELCEYVKSRLEVSDDLENVCNWVVDTCLHKGSRDNMSIVLVCFSNAPKVSDEAVKKDSELDKHLESRVEEIMEKSGEEGMPDLAHVMRILSAENIPNLPPGGGLAGKRNVIEAVYSRLNPHRESDGASDEAEESGSQGKLVEALRQMRINHRGNYRQLLEEMLTSYRLAKVEGEESPAEPAATATSSNSDAGNPVTMQESHTESESGLAELDSSNEDAGTKMSGEKI

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Bovine
100
Dog
100
Rabbit
100
Horse
86
Sheep
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O75688 As Substrate

Site PTM Type Enzyme
G2 Myristoylation
K12 Ubiquitination
K90 Ubiquitination
K103 Ubiquitination
K112 Ubiquitination
S121 Phosphorylation
S140 Phosphorylation
C163 S-Nitrosylation
C172 S-Nitrosylation
S195 Phosphorylation P17612 (PRKACA)
S265 Phosphorylation
S296 Phosphorylation
K314 Ubiquitination
K326 Ubiquitination
S343 Phosphorylation
Y367 Phosphorylation
S376 Phosphorylation
S386 Phosphorylation
K391 Ubiquitination
Y418 Phosphorylation

Research Backgrounds

Function:

Enzyme with a broad specificity. Dephosphorylates CDK2 and CDK6 in vitro. Dephosphorylates PRKAA1 and PRKAA2. Inhibits TBK1-mediated antiviral signaling by dephosphorylating it at 'Ser-172'. Plays an important role in the termination of TNF-alpha-mediated NF-kappa-B activation through dephosphorylating and inactivating IKBKB/IKKB.

PTMs:

Isgylation negatively regulates its activity.

N-myristoylation is essential for the recognition of its substrates for dephosphorylation.

Subcellular Location:

Cytoplasm>Cytosol. Membrane>Lipid-anchor.
Note: Weakly associates at the membrane and N-myristoylation mediates the membrane localization.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Highly expressed in heart and skeletal muscle.

Subunit Structure:

Monomer (By similarity). Interacts with PAK6. Interacts with the phosphorylated form of IKBKB/IKKB.

Family&Domains:

Belongs to the PP2C family.

Research Fields

· Environmental Information Processing > Signal transduction > MAPK signaling pathway.   (View pathway)

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