Product: SHIP1 Antibody
Catalog: AF0252
Description: Rabbit polyclonal antibody to SHIP1
Application: WB IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
Mol.Wt.: 100~160kD; 133kD(Calculated).
Uniprot: Q92835
RRID: AB_2833427

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 100ul $280 In stock
 200ul $350 In stock

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Product Info

WB 1:500-1:3000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Pig(100%), Zebrafish(88%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Xenopus(83%)
SHIP1 Antibody detects endogenous levels of total SHIP1.
Cite Format: Affinity Biosciences Cat# AF0252, RRID:AB_2833427.
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.


Inositol polyphosphate 5 phosphatase of 145kDa; 4; 5-trisphosphate 5-phosphatase 1; hp51CN; Inositol polyphosphate 5 phosphatase 145kDa; Inositol polyphosphate 5 phosphatase; Inositol polyphosphate-5-phosphatase of 145 kDa; INPP5D; MGC104855; MGC142140; MGC142142; p150Ship; Phosphatidylinositol 3,4,5 trisphosphate 5 phosphatase 1; Phosphatidylinositol-3; SH2 containing inositol phosphatase isoform b; SH2 domain containing inositol 5' phosphatase 1; SH2 domain containing inositol phosphatase 1; SH2 domain-containing inositol phosphatase 1; SH2 domain-containing inositol-5''-phosphatase 1; SHIP-1; SHIP1; SHIP1_HUMAN; Signaling inositol polyphosphate 5 phosphatase SIP 145; SIP-145; SIP145;



Specifically expressed in immune and hematopoietic cells. Expressed in bone marrow and blood cells. Levels vary considerably within this compartment. Present in at least 74% of immature CD34+ cells, whereas within the more mature population of CD33+ cells, it is present in only 10% of cells. Present in the majority of T-cells, while it is present in a minority of B-cells (at protein level).

SHIP an SH2-containing inositol phosphatase. A hemopoietic-specific phosphatase that regulates cell survival, growth, cell cycle arrest and apoptosis. Hydrolyzes Ins(1,3,4,5)P4 and PtdIns(3,4,5)P3. A cytosolic protein with a SH2 domain in its N-terminus and two NPXY Shc binding motifs at its C-terminus.



Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q92835 As Substrate

Site PTM Type Enzyme
K14 Ubiquitination
K24 Ubiquitination
S27 Phosphorylation
Y40 Phosphorylation
Y46 Phosphorylation
K78 Ubiquitination
K141 Ubiquitination
K182 Ubiquitination
K200 Ubiquitination
S205 Phosphorylation
T213 Phosphorylation
Y221 Phosphorylation
S243 Phosphorylation
S274 Phosphorylation
S289 Phosphorylation
S294 Phosphorylation
Y339 Phosphorylation
K365 Ubiquitination
K371 Acetylation
Y373 Phosphorylation
T423 Phosphorylation
Y556 Phosphorylation
K607 Ubiquitination
Y611 Phosphorylation
Y644 Phosphorylation
S699 Phosphorylation
S744 Phosphorylation
S754 Phosphorylation
S755 Phosphorylation
S772 Phosphorylation
K779 Ubiquitination
K786 Ubiquitination
Y796 Phosphorylation
Y834 Phosphorylation
T835 Phosphorylation
K859 Ubiquitination
K863 Ubiquitination
Y865 Phosphorylation
K869 Ubiquitination
K879 Ubiquitination
T880 Phosphorylation
K882 Ubiquitination
S886 Phosphorylation
K891 Ubiquitination
T908 Phosphorylation
Y915 Phosphorylation
S934 Phosphorylation
Y944 Phosphorylation
S951 Phosphorylation
S960 Phosphorylation
T963 Phosphorylation
S971 Phosphorylation
K974 Ubiquitination
S978 Phosphorylation
T979 Phosphorylation
T1003 Phosphorylation
Y1022 Phosphorylation
S1024 Phosphorylation
S1026 Phosphorylation
S1027 Phosphorylation
S1039 Phosphorylation
K1045 Ubiquitination
S1057 Phosphorylation
K1076 Ubiquitination
S1085 Phosphorylation
K1107 Ubiquitination
T1108 Phosphorylation
K1119 Ubiquitination
K1123 Ubiquitination
T1133 Phosphorylation
T1136 Phosphorylation
R1140 Methylation
S1147 Phosphorylation
S1156 Phosphorylation
K1157 Ubiquitination
Y1161 Phosphorylation
K1172 Ubiquitination

Research Backgrounds


Phosphatidylinositol (PtdIns) phosphatase that specifically hydrolyzes the 5-phosphate of phosphatidylinositol-3,4,5-trisphosphate (PtdIns(3,4,5)P3) to produce PtdIns(3,4)P2, thereby negatively regulating the PI3K (phosphoinositide 3-kinase) pathways. Able also to hydrolyzes the 5-phosphate of phosphatidylinositol-4,5-bisphosphate (PtdIns(4,5)P3) and inositol 1,3,4,5-tetrakisphosphate. Acts as a negative regulator of B-cell antigen receptor signaling. Mediates signaling from the FC-gamma-RIIB receptor (FCGR2B), playing a central role in terminating signal transduction from activating immune/hematopoietic cell receptor systems. Acts as a negative regulator of myeloid cell proliferation/survival and chemotaxis, mast cell degranulation, immune cells homeostasis, integrin alpha-IIb/beta-3 signaling in platelets and JNK signaling in B-cells. Regulates proliferation of osteoclast precursors, macrophage programming, phagocytosis and activation and is required for endotoxin tolerance. Involved in the control of cell-cell junctions, CD32a signaling in neutrophils and modulation of EGF-induced phospholipase C activity. Key regulator of neutrophil migration, by governing the formation of the leading edge and polarization required for chemotaxis. Modulates FCGR3/CD16-mediated cytotoxicity in NK cells. Mediates the activin/TGF-beta-induced apoptosis through its Smad-dependent expression.


Tyrosine phosphorylated by the members of the SRC family after exposure to a diverse array of extracellular stimuli such as cytokines, growth factors, antibodies, chemokines, integrin ligands and hypertonic and oxidative stress. Phosphorylated upon IgG receptor FCGR2B-binding.

Subcellular Location:

Cytoplasm. Cell membrane>Peripheral membrane protein. Membrane raft. Cytoplasm>Cytoskeleton. Membrane>Peripheral membrane protein.
Note: Translocates to the plasma membrane when activated, translocation is probably due to different mechanisms depending on the stimulus and cell type. Translocates from the cytoplasm to membrane ruffles in a FCGR3/CD16-dependent manner. Colocalizes with FC-gamma-RIIB receptor (FCGR2B) or FCGR3/CD16 at membrane ruffles. Tyrosine phosphorylation may also participate in membrane localization.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Specifically expressed in immune and hematopoietic cells. Expressed in bone marrow and blood cells. Levels vary considerably within this compartment. Present in at least 74% of immature CD34+ cells, whereas within the more mature population of CD33+ cells, it is present in only 10% of cells. Present in the majority of T-cells, while it is present in a minority of B-cells (at protein level).

Subunit Structure:

Interacts with tyrosine phosphorylated form of SHC1. Interacts with tyrosine phosphorylated form of DOK1. Interacts with tyrosine phosphorylated form of DOK3 (By similarity). Interacts with tyrosine phosphorylated form of SLAMF1/CD150. Interacts with PTPN11 in response to IL-3 (By similarity). Interacts with receptor EPOR (By similarity). Interacts with receptors MS4A2/FCER1B and FCER1G (By similarity). Interacts with receptors FCGR2B and FCGR3 (By similarity). Interacts with receptor FCGR2A, leading to regulate gene expression during the phagocytic process (By similarity). Interacts with GRB2. Interacts with PLCG1 (By similarity). Interacts with tyrosine kinases SRC and TEC (By similarity). Interacts with c-Met/MET (By similarity). Interacts with MILR1 (tyrosine-phosphorylated) (By similarity). Can weakly interact (via NPXY motif 2) with DAB2 (via PID domain); the interaction is impaired by tyrosine phosphorylation of the NPXY motif (By similarity). Interacts with FCRL3 and FCRL6 (tyrosine phosphorylated form).


The SH2 domain interacts with tyrosine phosphorylated forms of proteins such as SHC1 or PTPN11/SHP-2. It competes with that of GRB2 for binding to phosphorylated SHC1 to inhibit the Ras pathway. It is also required for tyrosine phosphorylation (By similarity).

The NPXY sequence motif found in many tyrosine-phosphorylated proteins is required for the specific binding of the PID domain.

Belongs to the inositol 1,4,5-trisphosphate 5-phosphatase family.

Research Fields

· Environmental Information Processing > Signal transduction > Phosphatidylinositol signaling system.

· Metabolism > Carbohydrate metabolism > Inositol phosphate metabolism.

· Organismal Systems > Immune system > B cell receptor signaling pathway.   (View pathway)

· Organismal Systems > Immune system > Fc epsilon RI signaling pathway.   (View pathway)

· Organismal Systems > Immune system > Fc gamma R-mediated phagocytosis.   (View pathway)

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