SHPS1 Antibody - #AF0253
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Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# AF0253, RRID:AB_2833428.
Fold/Unfold
Signal regulatory protein alpha type 1; Bit; Brain Ig like molecule with tyrosine based activation motifs; Brain Ig-like molecule with tyrosine-based activation motifs; Brain immunoglobulin like molecule with tyrosine based activation motifs; CD172 antigen like family member A; CD172 antigen-like family member A; CD172a; CD172a antigen; Inhibitory receptor SHPS-1; Macrophage fusion receptor; MFR; MYD 1; Myd 1 antigen; MyD-1 antigen; p84; Protein tyrosine phosphatase non receptor type substrate 1; PTPNS1; SHP substrate 1; SHPS-1; SHPS1; SHPS1_HUMAN; Signal regulatory protein alpha 2; Signal regulatory protein alpha 3; Signal regulatory protein alpha; Signal regulatory protein alpha type 2; Signal-regulatory protein alpha-1; Signal-regulatory protein alpha-2; Signal-regulatory protein alpha-3; SIRP; Sirp-alpha-1; Sirp-alpha-2; Sirp-alpha-3; SIRPA; SIRPalpha; SIRPalpha1; SIRPalpha2; SIRPalpha3; Tyrosine phosphatase SHP substrate 1; Tyrosine protein phosphatase non receptor type substrate 1; Tyrosine-protein phosphatase non-receptor type substrate 1;
Immunogens
Ubiquitous. Highly expressed in brain. Detected on myeloid cells, but not T-cells. Detected at lower levels in heart, placenta, lung, testis, ovary, colon, liver, small intestine, prostate, spleen, kidney, skeletal muscle and pancreas.
- P78324 SHPS1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MEPAGPAPGRLGPLLCLLLAASCAWSGVAGEEELQVIQPDKSVLVAAGETATLRCTATSLIPVGPIQWFRGAGPGRELIYNQKEGHFPRVTTVSDLTKRNNMDFSIRIGNITPADAGTYYCVKFRKGSPDDVEFKSGAGTELSVRAKPSAPVVSGPAARATPQHTVSFTCESHGFSPRDITLKWFKNGNELSDFQTNVDPVGESVSYSIHSTAKVVLTREDVHSQVICEVAHVTLQGDPLRGTANLSETIRVPPTLEVTQQPVRAENQVNVTCQVRKFYPQRLQLTWLENGNVSRTETASTVTENKDGTYNWMSWLLVNVSAHRDDVKLTCQVEHDGQPAVSKSHDLKVSAHPKEQGSNTAAENTGSNERNIYIVVGVVCTLLVALLMAALYLVRIRQKKAQGSTSSTRLHEPEKNAREITQDTNDITYADLNLPKGKKPAPQAAEPNNHTEYASIQTSPQPASEDTLTYADLDMVHLNRTPKQPAPKPEPSFSEYASVQVPRK
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - P78324 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K83 | Ubiquitination | Uniprot | |
| K98 | Ubiquitination | Uniprot | |
| K183 | Ubiquitination | Uniprot | |
| S192 | Phosphorylation | Uniprot | |
| S204 | Phosphorylation | Uniprot | |
| N245 | N-Glycosylation | Uniprot | |
| N270 | N-Glycosylation | Uniprot | |
| N292 | N-Glycosylation | Uniprot | |
| T408 | Phosphorylation | Uniprot | |
| K415 | Ubiquitination | Uniprot | |
| T428 | Phosphorylation | Uniprot | |
| Y429 | Phosphorylation | P42679 (MATK) | Uniprot |
| K436 | Ubiquitination | Uniprot | |
| Y453 | Phosphorylation | P42679 (MATK) | Uniprot |
| S464 | Phosphorylation | Uniprot | |
| T467 | Phosphorylation | Uniprot | |
| T469 | Phosphorylation | Uniprot | |
| Y470 | Phosphorylation | P42679 (MATK) , P08069 (IGF1R) | Uniprot |
| K483 | Acetylation | Uniprot | |
| S494 | Phosphorylation | Uniprot | |
| Y496 | Phosphorylation | P42679 (MATK) , P08069 (IGF1R) | Uniprot |
Research Backgrounds
Immunoglobulin-like cell surface receptor for CD47. Acts as docking protein and induces translocation of PTPN6, PTPN11 and other binding partners from the cytosol to the plasma membrane. Supports adhesion of cerebellar neurons, neurite outgrowth and glial cell attachment. May play a key role in intracellular signaling during synaptogenesis and in synaptic function (By similarity). Involved in the negative regulation of receptor tyrosine kinase-coupled cellular responses induced by cell adhesion, growth factors or insulin. Mediates negative regulation of phagocytosis, mast cell activation and dendritic cell activation. CD47 binding prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells.
N-glycosylated.
Phosphorylated on tyrosine residues in response to stimulation with EGF, growth hormone, insulin and PDGF. Dephosphorylated by PTPN11.
Membrane>Single-pass type I membrane protein.
Ubiquitous. Highly expressed in brain. Detected on myeloid cells, but not T-cells. Detected at lower levels in heart, placenta, lung, testis, ovary, colon, liver, small intestine, prostate, spleen, kidney, skeletal muscle and pancreas.
Binds PTPN11 when tyrosine-phosphorylated, except in macrophages, where it primarily binds PTPN6. Binds GRB2 in vitro. Binds FGR (By similarity). Binds JAK2 irrespective of its phosphorylation status and forms a stable complex. Binds SCAP1 and/or SCAP2. The resulting complex recruits FYB1. Binds PTK2B.
Research Fields
· Organismal Systems > Development > Osteoclast differentiation. (View pathway)
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