Phospho-Nanog (Thr280) Antibody - #AF3535

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Product: Phospho-Nanog (Thr280) Antibody
Catalog: AF3535
Description: Rabbit polyclonal antibody to Phospho-Nanog (Thr280)
Application: IHC IF/ICC
Reactivity: Human, Mouse, Rat
Mol.Wt.: 35kD(Calculated).
Uniprot: Q9H9S0
RRID: AB_2846849

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Related Downloads
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Clonality:
Polyclonal
Specificity:
Phospho-Nanog (Thr280) Antibody detects endogenous levels of Nanog only when phosphorylated at Thr280.
RRID:
AB_2846849
Cite Format: Affinity Biosciences Cat# AF3535, RRID:AB_2846849.
Conjugate:
Unconjugated.
Purification:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Embryonic stem cell specific homeobox protein (Nanog); ENK; FLJ12581; hNanog; Homeobox protein NANOG; Homeobox transcription factor Nanog; homeobox transcription factor Nanog-delta 48; NANOG; Nanog homeobox; NANOG_HUMAN;

Immunogens

Immunogen:

A synthesized peptide derived from human Nanog around the phosphorylation site of Thr280.

Uniprot:

Q9H9S0(NANOG_HUMAN) >>Visit HPA database.

Gene(ID):
NANOG(79923)
Expression:
Q9H9S0 NANOG_HUMAN:

Expressed in testicular carcinoma and derived germ cell tumors (at protein level). Expressed in fetal gonads, ovary and testis. Also expressed in ovary teratocarcinoma cell line and testicular embryonic carcinoma. Not expressed in many somatic organs and oocytes.

Sequence:
MSVDPACPQSLPCFEASDCKESSPMPVICGPEENYPSLQMSSAEMPHTETVSPLPSSMDLLIQDSPDSSTSPKGKQPTSAEKSVAKKEDKVPVKKQKTRTVFSSTQLCVLNDRFQRQKYLSLQQMQELSNILNLSYKQVKTWFQNQRMKSKRWQKNNWPKNSNGVTQKASAPTYPSLYSSYHQGCLVNPTGNLPMWSNQTWNNSTWSNQTQNIQSWSNHSWNTQTWCTQSWNNQAWNSPFYNCGEESLQSCMQFQPNSPASDLEAALEAAGEGLNVIQQTTRYFSTPQTMDLFLNYSMNMQPEDV

PTMs - Q9H9S0 As Substrate

Site PTM Type Enzyme
S2 Phosphorylation
Y35 Phosphorylation Q05397 (PTK2)
T50 Phosphorylation
S52 Phosphorylation P28482 (MAPK1)
S56 Phosphorylation
S57 Phosphorylation
S65 Phosphorylation
T70 Phosphorylation
S71 Phosphorylation P28482 (MAPK1) , P06493 (CDK1)
T78 Phosphorylation Q02156 (PRKCE) , P28482 (MAPK1)
S79 Phosphorylation Q02156 (PRKCE)
S135 Phosphorylation Q02156 (PRKCE)
Y174 Phosphorylation Q05397 (PTK2)
T200 Phosphorylation Q02156 (PRKCE)
S258 Phosphorylation
T280 Phosphorylation Q02156 (PRKCE)
T286 Phosphorylation
T289 Phosphorylation

Research Backgrounds

Function:

Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes. Acts as a transcriptional activator or repressor. Binds optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3'. Binds to the POU5F1/OCT4 promoter. Able to autorepress its expression in differentiating (ES) cells: binds to its own promoter following interaction with ZNF281/ZFP281, leading to recruitment of the NuRD complex and subsequent repression of expression. When overexpressed, promotes cells to enter into S phase and proliferation.

Subcellular Location:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in testicular carcinoma and derived germ cell tumors (at protein level). Expressed in fetal gonads, ovary and testis. Also expressed in ovary teratocarcinoma cell line and testicular embryonic carcinoma. Not expressed in many somatic organs and oocytes.

Subunit Structure:

Interacts with SMAD1 and SALL4. Interacts with ZNF281/ZFP281 (By similarity). Interacts with PCGF1. Interacts with ESRRB; reciprocally modulates their transcriptional activities (By similarity).

Family&Domains:

Belongs to the Nanog homeobox family.

Research Fields

· Cellular Processes > Cellular community - eukaryotes > Signaling pathways regulating pluripotency of stem cells.   (View pathway)

· Human Diseases > Cancers: Overview > Proteoglycans in cancer.

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