Phospho-SMAD6 (Ser435) Antibody - #AF3769

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Product: Phospho-SMAD6 (Ser435) Antibody
Catalog: AF3769
Description: Rabbit polyclonal antibody to Phospho-SMAD6 (Ser435)
Application: ELISA(peptide)
Cited expt.:
Reactivity: Human, Mouse, Rat
Mol.Wt.: 53kD(Calculated).
Uniprot: O43541
RRID: AB_2847083

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MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
ELISA(peptide) 1:20000-1:40000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Clonality:
Polyclonal
Specificity:
Phospho-SMAD6 (Ser435) Antibody detects endogenous levels of SMAD6 only when phosphorylated at Ser435.
RRID:
AB_2847083
Cite Format: Affinity Biosciences Cat# AF3769, RRID:AB_2847083.
Conjugate:
Unconjugated.
Purification:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

hSMAD6; MAD homolog 6; MADH6; MADH7; Mothers against decapentaplegic homolog 6; Mothers against DPP homolog 6; SMAD 6; SMAD family member 6; SMAD mothers against DPP homolog 6; Smad6; SMAD6_HUMAN;

Immunogens

Immunogen:

A synthesized peptide derived from human SMAD6 around the phosphorylation site of Ser435.

Uniprot:

O43541(SMAD6_HUMAN) >>Visit HPA database.

Gene(ID):
SMAD6(4091)
Expression:
O43541 SMAD6_HUMAN:

Ubiquitous in various organs, with higher levels in lung. Isoform B is up-regulated in diseased heart tissue.

Sequence:
MFRSKRSGLVRRLWRSRVVPDREEGGSGGGGGGDEDGSLGSRAEPAPRAREGGGCGRSEVRPVAPRRPRDAVGQRGAQGAGRRRRAGGPPRPMSEPGAGAGSSLLDVAEPGGPGWLPESDCETVTCCLFSERDAAGAPRDASDPLAGAALEPAGGGRSREARSRLLLLEQELKTVTYSLLKRLKERSLDTLLEAVESRGGVPGGCVLVPRADLRLGGQPAPPQLLLGRLFRWPDLQHAVELKPLCGCHSFAAAADGPTVCCNPYHFSRLCGPESPPPPYSRLSPRDEYKPLDLSDSTLSYTETEATNSLITAPGEFSDASMSPDATKPSHWCSVAYWEHRTRVGRLYAVYDQAVSIFYDLPQGSGFCLGQLNLEQRSESVRRTRSKIGFGILLSKEPDGVWAYNRGEHPIFVNSPTLDAPGGRALVVRKVPPGYSIKVFDFERSGLQHAPEPDAADGPYDPNSVRISFAKGWGPCYSRQFITSCPCWLEILLNNPR

Research Backgrounds

Function:

Acts as a mediator of TGF-beta and BMP antiflammatory activity. Suppresses IL1R-TLR signaling through its direct interaction with PEL1, preventing NF-kappa-B activation, nuclear transport and NF-kappa-B-mediated expression of proinflammatory genes. May block the BMP-SMAD1 signaling pathway by competing with SMAD4 for receptor-activated SMAD1-binding. Binds to regulatory elements in target promoter regions.

PTMs:

Phosphorylated by BMP type 1 receptor kinase and by PRKX.

Monoubiquitinated at Lys-173 by the E2/E3 hybrid ubiquitin-protein ligase UBE2O, leading to reduced binding affinity for the activated BMP type I receptor ACVR1/ALK2, thereby enhancing BMP7 and regulating adipocyte differentiation. Ubiquitinated by WWP1 (By similarity). Ubiquitinated by RNF165, promoting proteasomal degradation, leading to enhance the BMP-Smad signaling (By similarity).

Arginine methylation by PRMT1, which is recruited by BMPR2, initiates BMP-Induced signaling and induces dissociation from the BMPR1B receptor at the cell surface leading to derepress downstream Smad1/Smad5 signaling.

Subcellular Location:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Ubiquitous in various organs, with higher levels in lung. Isoform B is up-regulated in diseased heart tissue.

Family&Domains:

Belongs to the dwarfin/SMAD family.

Research Fields

· Environmental Information Processing > Signal transduction > TGF-beta signaling pathway.   (View pathway)

References

1). FGF receptor inhibitor BGJ398 partially rescues osteoarthritis-like phenotype in older high molecular weight FGF2 transgenic mice via multiple mechanisms. Scientific Reports, 2022 (PubMed: 36153352) [IF=3.8]

Application: IHC    Species: Mouse    Sample:

Expression of pSMAD1/5/8, pSMAD6, RUNX2 and pERK in knee articular cartilage of 9.5-month-old Vector-Control and HMWTgFGF2 mice treated with BGJ398. Eight month-old Vector-Control (Vec) and HMWTgFGF2 (HMW) female mice were sq injected with vehicle or BGJ398 5 days/week for 6 weeks. At the end of the experiment (9.5 month old) knee joints were collected for IHC staining. Representative immunohistochemical staining images for (A) pSMAD1/5/8, (B) pSMAD6, (C) RUNX2, and (D) pERK. Quantitative analysis of percent-positive staining area for (E) pSMAD1/5/8, (F) pSMAD6, (G) RUNX2, and (H) pERK in articular cartilage. Data are shown as mean and individual points. n = 7–9 mice/group for (E, G, H); n = 4 mice /group for (F). *p 

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