Product: Phospho-SMAD6 (Ser435) Antibody
Catalog: AF3769
Description: Rabbit polyclonal antibody to Phospho-SMAD6 (Ser435)
Application: ELISA(peptide)
Cited expt.:
Reactivity: Human, Mouse, Rat
Mol.Wt.: 53kD(Calculated).
Uniprot: O43541
RRID: AB_2847083

View similar products>>

   Size Price Inventory
 100ul $350 In stock
 200ul $450 In stock

Lead Time: Same day delivery

For pricing and ordering contact:
Local distributors

Product Info

Source:
Rabbit
Application:
ELISA(peptide) 1:20000-1:40000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Clonality:
Polyclonal
Specificity:
Phospho-SMAD6 (Ser435) Antibody detects endogenous levels of SMAD6 only when phosphorylated at Ser435.
RRID:
AB_2847083
Cite Format: Affinity Biosciences Cat# AF3769, RRID:AB_2847083.
Conjugate:
Unconjugated.
Purification:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

hSMAD6; MAD homolog 6; MADH6; MADH7; Mothers against decapentaplegic homolog 6; Mothers against DPP homolog 6; SMAD 6; SMAD family member 6; SMAD mothers against DPP homolog 6; Smad6; SMAD6_HUMAN;

Immunogens

Immunogen:

A synthesized peptide derived from human SMAD6 around the phosphorylation site of Ser435.

Uniprot:
Gene(ID):
Expression:
O43541 SMAD6_HUMAN:

Ubiquitous in various organs, with higher levels in lung. Isoform B is up-regulated in diseased heart tissue.

Sequence:
MFRSKRSGLVRRLWRSRVVPDREEGGSGGGGGGDEDGSLGSRAEPAPRAREGGGCGRSEVRPVAPRRPRDAVGQRGAQGAGRRRRAGGPPRPMSEPGAGAGSSLLDVAEPGGPGWLPESDCETVTCCLFSERDAAGAPRDASDPLAGAALEPAGGGRSREARSRLLLLEQELKTVTYSLLKRLKERSLDTLLEAVESRGGVPGGCVLVPRADLRLGGQPAPPQLLLGRLFRWPDLQHAVELKPLCGCHSFAAAADGPTVCCNPYHFSRLCGPESPPPPYSRLSPRDEYKPLDLSDSTLSYTETEATNSLITAPGEFSDASMSPDATKPSHWCSVAYWEHRTRVGRLYAVYDQAVSIFYDLPQGSGFCLGQLNLEQRSESVRRTRSKIGFGILLSKEPDGVWAYNRGEHPIFVNSPTLDAPGGRALVVRKVPPGYSIKVFDFERSGLQHAPEPDAADGPYDPNSVRISFAKGWGPCYSRQFITSCPCWLEILLNNPR

Research Backgrounds

Function:

Acts as a mediator of TGF-beta and BMP antiflammatory activity. Suppresses IL1R-TLR signaling through its direct interaction with PEL1, preventing NF-kappa-B activation, nuclear transport and NF-kappa-B-mediated expression of proinflammatory genes. May block the BMP-SMAD1 signaling pathway by competing with SMAD4 for receptor-activated SMAD1-binding. Binds to regulatory elements in target promoter regions.

PTMs:

Phosphorylated by BMP type 1 receptor kinase and by PRKX.

Monoubiquitinated at Lys-173 by the E2/E3 hybrid ubiquitin-protein ligase UBE2O, leading to reduced binding affinity for the activated BMP type I receptor ACVR1/ALK2, thereby enhancing BMP7 and regulating adipocyte differentiation. Ubiquitinated by WWP1 (By similarity). Ubiquitinated by RNF165, promoting proteasomal degradation, leading to enhance the BMP-Smad signaling (By similarity).

Arginine methylation by PRMT1, which is recruited by BMPR2, initiates BMP-Induced signaling and induces dissociation from the BMPR1B receptor at the cell surface leading to derepress downstream Smad1/Smad5 signaling.

Subcellular Location:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Ubiquitous in various organs, with higher levels in lung. Isoform B is up-regulated in diseased heart tissue.

Family&Domains:

Belongs to the dwarfin/SMAD family.

Research Fields

· Environmental Information Processing > Signal transduction > TGF-beta signaling pathway.   (View pathway)

References

1). FGF receptor inhibitor BGJ398 partially rescues osteoarthritis-like phenotype in older high molecular weight FGF2 transgenic mice via multiple mechanisms. Scientific Reports, 2022 (PubMed: 36153352) [IF=3.8]

Application: IHC    Species: Mouse    Sample:

Expression of pSMAD1/5/8, pSMAD6, RUNX2 and pERK in knee articular cartilage of 9.5-month-old Vector-Control and HMWTgFGF2 mice treated with BGJ398. Eight month-old Vector-Control (Vec) and HMWTgFGF2 (HMW) female mice were sq injected with vehicle or BGJ398 5 days/week for 6 weeks. At the end of the experiment (9.5 month old) knee joints were collected for IHC staining. Representative immunohistochemical staining images for (A) pSMAD1/5/8, (B) pSMAD6, (C) RUNX2, and (D) pERK. Quantitative analysis of percent-positive staining area for (E) pSMAD1/5/8, (F) pSMAD6, (G) RUNX2, and (H) pERK in articular cartilage. Data are shown as mean and individual points. n = 7–9 mice/group for (E, G, H); n = 4 mice /group for (F). *p 

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.