Perilipin-1 Antibody | Affinity Biosciences

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Perilipin-1 Antibody - Western blot analysis of extracts from EC304 cells(heat-shock treatment), using Perilipin-1 Antibody at 1/1000 dilution.

Product:	Perilipin-1 Antibody
Catalog:	AF6834
Description:	Rabbit polyclonal antibody to Perilipin-1
Application:	WB ELISA(peptide)
Reactivity:	Human, Mouse, Rat
Mol.Wt.:	56kD(Calculated).
Uniprot:	O60240
RRID:	AB_2847557

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, ELISA(peptide) 1:20000-1:40000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Clonality:

Polyclonal

Specificity:

Perilipin-1 Antibody detects endogenous levels of total Perilipin-1.

RRID:

AB_2847557
Cite Format: Affinity Biosciences Cat# AF6834, RRID:AB_2847557.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Lipid droplet associated protein; Lipid droplet-associated protein; PERI; Perilipin A; Perilipin; Perilipin-1; PerilipinA; PLIN; PLIN1; PLIN1_HUMAN;

Immunogens

Immunogen:

A synthesized peptide derived from human Perilipin-1.

Uniprot:

O60240(PLIN1_HUMAN) >>Visit HPA database.

Gene(ID):

PLIN1(5346)

Expression:

O60240 PLIN1_HUMAN:

Detected in adipocytes from white adipose tissue (at protein level) (PubMed:27832861). Detected in visceral adipose tissue and mammary gland (PubMed:9521880).

Sequence:

O60240 PLIN1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MAVNKGLTLLDGDLPEQENVLQRVLQLPVVSGTCECFQKTYTSTKEAHPLVASVCNAYEKGVQSASSLAAWSMEPVVRRLSTQFTAANELACRGLDHLEEKIPALQYPPEKIASELKDTISTRLRSARNSISVPIASTSDKVLGAALAGCELAWGVARDTAEFAANTRAGRLASGGADLALGSIEKVVEYLLPPDKEESAPAPGHQQAQKSPKAKPSLLSRVGALTNTLSRYTVQTMARALEQGHTVAMWIPGVVPLSSLAQWGASVAMQAVSRRRSEVRVPWLHSLAAAQEEDHEDQTDTEGEDTEEEEELETEENKFSEVAALPGPRGLLGGVAHTLQKTLQTTISAVTWAPAAVLGMAGRVLHLTPAPAVSSTKGRAMSLSDALKGVTDNVVDTVVHYVPLPRLSLMEPESEFRDIDNPPAEVERREAERRASGAPSAGPEPAPRLAQPRRSLRSAQSPGAPPGPGLEDEVATPAAPRPGFPAVPREKPKRRVSDSFFRPSVMEPILGRTHYSQLRKKS

Research Backgrounds

O60240_PLIN1_HUMAN

Function:

Modulator of adipocyte lipid metabolism. Coats lipid storage droplets to protect them from breakdown by hormone-sensitive lipase (HSL). Its absence may result in leanness. Plays a role in unilocular lipid droplet formation by activating CIDEC. Their interaction promotes lipid droplet enlargement and directional net neutral lipid transfer. May modulate lipolysis and triglyceride levels.

PTMs:

Major cAMP-dependent protein kinase-substrate in adipocytes, also dephosphorylated by PP1. When phosphorylated, may be maximally sensitive to HSL and when unphosphorylated, may play a role in the inhibition of lipolysis, by acting as a barrier in lipid droplet (By similarity).

Subcellular Location:

Endoplasmic reticulum. Lipid droplet.
Note: Lipid droplet surface-associated.

Tissue Specificity:

Detected in adipocytes from white adipose tissue (at protein level). Detected in visceral adipose tissue and mammary gland.

Family&Domains:

Belongs to the perilipin family.

Research Fields

· Environmental Information Processing > Signal transduction > Apelin signaling pathway. (View pathway)

· Organismal Systems > Endocrine system > PPAR signaling pathway.

· Organismal Systems > Endocrine system > Regulation of lipolysis in adipocytes.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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Perilipin-1 Antibody - #AF6834