Product: VAV1 Antibody
Catalog: AF6182
Description: Rabbit polyclonal antibody to VAV1
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 95kDa; 98kD(Calculated).
Uniprot: P15498
RRID: AB_2835065

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 100ul $280 In stock
 200ul $350 In stock

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Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(91%), Horse(100%), Sheep(91%), Rabbit(100%), Dog(100%)
Clonality:
Polyclonal
Specificity:
VAV1 Antibody detects endogenous levels of total VAV1.
RRID:
AB_2835065
Cite Format: Affinity Biosciences Cat# AF6182, RRID:AB_2835065.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Oncogene vav; p95Vav; Proto-oncogene vav; Protooncogene vav; VAV 1; VAV 1 oncogene; VAV; Vav proto oncogene; VAV_HUMAN; VAV1;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
P15498 VAV_HUMAN:

Widely expressed in hematopoietic cells but not in other cell types.

Description:
The protein encoded by this proto-oncogene is a member of the Dbl family of guanine nucleotide exchange factors (GEF) for the Rho family of GTP binding proteins.
Sequence:
MELWRQCTHWLIQCRVLPPSHRVTWDGAQVCELAQALRDGVLLCQLLNNLLPHAINLREVNLRPQMSQFLCLKNIRTFLSTCCEKFGLKRSELFEAFDLFDVQDFGKVIYTLSALSWTPIAQNRGIMPFPTEEESVGDEDIYSGLSDQIDDTVEEDEDLYDCVENEEAEGDEIYEDLMRSEPVSMPPKMTEYDKRCCCLREIQQTEEKYTDTLGSIQQHFLKPLQRFLKPQDIEIIFINIEDLLRVHTHFLKEMKEALGTPGAANLYQVFIKYKERFLVYGRYCSQVESASKHLDRVAAAREDVQMKLEECSQRANNGRFTLRDLLMVPMQRVLKYHLLLQELVKHTQEAMEKENLRLALDAMRDLAQCVNEVKRDNETLRQITNFQLSIENLDQSLAHYGRPKIDGELKITSVERRSKMDRYAFLLDKALLICKRRGDSYDLKDFVNLHSFQVRDDSSGDRDNKKWSHMFLLIEDQGAQGYELFFKTRELKKKWMEQFEMAISNIYPENATANGHDFQMFSFEETTSCKACQMLLRGTFYQGYRCHRCRASAHKECLGRVPPCGRHGQDFPGTMKKDKLHRRAQDKKRNELGLPKMEVFQEYYGLPPPPGAIGPFLRLNPGDIVELTKAEAEQNWWEGRNTSTNEIGWFPCNRVKPYVHGPPQDLSVHLWYAGPMERAGAESILANRSDGTFLVRQRVKDAAEFAISIKYNVEVKHIKIMTAEGLYRITEKKAFRGLTELVEFYQQNSLKDCFKSLDTTLQFPFKEPEKRTISRPAVGSTKYFGTAKARYDFCARDRSELSLKEGDIIKILNKKGQQGWWRGEIYGRVGWFPANYVEEDYSEYC

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Dog
100
Rabbit
100
Bovine
91
Sheep
91
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P15498 As Substrate

Site PTM Type Enzyme
K73 Ubiquitination
K85 Ubiquitination
K89 Ubiquitination
Y110 Phosphorylation
S113 Phosphorylation
Y142 Phosphorylation P06239 (LCK)
Y160 Phosphorylation P06239 (LCK)
Y174 Phosphorylation P43405 (SYK) , P06239 (LCK)
S180 Phosphorylation
K188 Ubiquitination
Y192 Phosphorylation
K194 Acetylation
K194 Ubiquitination
K208 Ubiquitination
S215 Phosphorylation
K222 Acetylation
K222 Ubiquitination
K252 Acetylation
K255 Ubiquitination
Y267 Phosphorylation P00519 (ABL1)
Y280 Phosphorylation
S285 Phosphorylation
K292 Ubiquitination
K307 Ubiquitination
K335 Ubiquitination
K345 Acetylation
K345 Ubiquitination
K353 Ubiquitination
K374 Ubiquitination
S396 Phosphorylation
K404 Ubiquitination
K410 Ubiquitination
K429 Ubiquitination
K435 Ubiquitination
K444 Ubiquitination
K465 Ubiquitination
K487 Acetylation
Y541 Phosphorylation
K555 Ubiquitination
T574 Phosphorylation
K576 Ubiquitination
K588 Ubiquitination
Y603 Phosphorylation
K629 Ubiquitination
S683 Phosphorylation
K700 Ubiquitination
K716 Acetylation
K716 Ubiquitination
K732 Acetylation
K732 Ubiquitination
K733 Acetylation
Y745 Phosphorylation
K751 Ubiquitination
K755 Ubiquitination
K766 Ubiquitination
K770 Ubiquitination
S774 Phosphorylation
S780 Phosphorylation
K782 Ubiquitination
Y783 Phosphorylation
T786 Phosphorylation
K788 Ubiquitination
Y791 Phosphorylation
K804 Ubiquitination
K810 Ubiquitination
K815 Ubiquitination
Y826 Phosphorylation
Y836 Phosphorylation
Y841 Phosphorylation
S842 Phosphorylation
Y844 Phosphorylation

Research Backgrounds

Function:

Couples tyrosine kinase signals with the activation of the Rho/Rac GTPases, thus leading to cell differentiation and/or proliferation.

PTMs:

Phosphorylated on tyrosine residues by HCK in response to IFNG and bacterial lipopolysaccharide (LPS) (By similarity). Phosphorylated by FYN.

Tissue Specificity:

Widely expressed in hematopoietic cells but not in other cell types.

Subunit Structure:

Interacts with SHB. Interacts with APS, GRB2, GRB3, DOCK2, SLA, TEC and ZNF655/VIK. Interacts with SIAH2; without leading to its degradation. Associates with BLNK, PLCG1, GRB2 and NCK1 in a B-cell antigen receptor-dependent fashion. Interacts with CBLB; which inhibits tyrosine phosphorylation and down-regulates activity. May interact with CCPG1. Interacts with CLNK. Interacts with THEMIS2 (By similarity). Interacts with NEK3 and this interaction is prolactin-dependent. Interacts with ITK. Interacts with PTK2B/PYK2 (By similarity). Interacts with HCK. Interacts with PTK2B/PYK2. Interacts (via SH2 domain) with SYK. Interacts with ANKRD54. Interacts with CD6 (By similarity).

Family&Domains:

The DH domain is involved in interaction with CCPG1.

Research Fields

· Cellular Processes > Cellular community - eukaryotes > Focal adhesion.   (View pathway)

· Cellular Processes > Cell motility > Regulation of actin cytoskeleton.   (View pathway)

· Environmental Information Processing > Signal transduction > cAMP signaling pathway.   (View pathway)

· Organismal Systems > Immune system > Chemokine signaling pathway.   (View pathway)

· Organismal Systems > Immune system > Natural killer cell mediated cytotoxicity.   (View pathway)

· Organismal Systems > Immune system > T cell receptor signaling pathway.   (View pathway)

· Organismal Systems > Immune system > B cell receptor signaling pathway.   (View pathway)

· Organismal Systems > Immune system > Fc epsilon RI signaling pathway.   (View pathway)

· Organismal Systems > Immune system > Fc gamma R-mediated phagocytosis.   (View pathway)

· Organismal Systems > Immune system > Leukocyte transendothelial migration.   (View pathway)

References

1). Jing Qiu et al. Vav1 promotes inflammation and neuronal apoptosis in cerebral ischemia/reperfusion injury by upregulating microglial and NLRP3 inflammasome activation. Neural Regeneration Research 2023 Nov;18(2436-2442) (PubMed: 37282474) [IF=6.1]

Application: WB    Species: Rat    Sample:

Figure 1 Increased Vav1 expression in the ischemic penumbra of MCAO/R rats.(A) Vav1 mRNA expression in MCAO/R rats according to the GEO database GSE163614. (B) Vav1 mRNA expression (normalized to the sham group) in the ischemic penumbra of MCAO/R rats. (C) Normalized Vav1 protein expression in the ischemic penumbra of MCAO/R rats. (D) Cerebral infarction area expressed as a percentage of total brain area, as determined by TTC staining. Data are expressed as the mean ± SD (n = 6), and were analyzed by unpaired t-test. The experiments were repeated three times. GEO: Gene expression omnibus; MCAO/R: middle cerebral artery occlusion/reperfusion; TTC staining: triphenyltetrazolium chloride staining; Vav1: Vav guanine nucleotide exchange factor 1. Source Vav1 promotes inflammation and neuronal apoptosis in cerebral ischemia/reperfusion injury by upregulating microglial and NLRP3 inflammasome activation Neural Regeneration Research18(11):2436-2442, November 2023.

Application: IF/ICC    Species: Rat    Sample:

Figure 4 Vav1 knockdown represses the activation of microglia and NLRP3 inflammasome in the ischemic penumbra of MCAO/R rats.(A) Double immunofluorescence staining for Vav1 (FITC, green) and Iba-1 (Cy3, red) in the ischemic penumbra area in MCAO/R rats. MCAO/R rats showed enhanced expression of Vav1 and Iba-1 compared with the sham group, but inhibition of Vav1 expression decreased the levels of both proteins. (B) Quantification of Vav1/Iba-1 double positive cells. (C) Double immunofluorescence staining for NLRP3 (FITC, green) and Iba-1 (Cy3, red) in the ischemic penumbra area in MCAO/R rats. MCAO/R rats showed enhanced expression of Iba-1 and NLRP3 compared with the sham group, but inhibition of Vav1 expression decreased the levels of both proteins. Nuclei were stained with DAPI (blue). Scale bars: 50 μm. (D) Quantification of NLRP3 and Iba-1 double positive cells. Data are expressed as the mean ± SD (n = 6), and were analyzed by one-way analysis of variance followed by Tukey’s multiple comparisons test. Cy3: Cyanine 3; DAPI: 4′,6-diamidino-2-phenylindole; FITC: fluorescein isothiocyanate; MCAO/R: middle cerebral artery occlusion/reperfusion; NLRP3: NOD-like receptor pyrin 3; Vav1: Vav guanine nucleotide exchange factor 1. Source Vav1 promotes inflammation and neuronal apoptosis in cerebral ischemia/reperfusion injury by upregulating microglial and NLRP3 inflammasome activation Neural Regeneration Research18(11):2436-2442, November 2023. Source Vav1 promotes inflammation and neuronal apoptosis in cerebral ischemia/reperfusion injury by upregulating microglial and NLRP3 inflammasome activation Neural Regeneration Research18(11):2436-2442, November 2023.

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